Mouse Genome Informatics
hm1
    Pdx1tm3Cvw/Pdx1tm3Cvw
involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice survive at least 7 days, but none reach weaning age

endocrine/exocrine glands
• dorsal pancreas is hypoplastic
• glucagon- and insulin-producing cells are decreased in number
• dorsal pancreatic remnant dorsal to duodenum forms as cystic structure lined with simple cuboidal epithelium

digestive/alimentary system
• GIP-producing cell numbers are reduced compared to wild-type controls
• pancreatic endocrine cell differentiation is defective; all four major endocrine cell types are detected, but only as small clusters or single cells rather than tightly apposed ductal structures


Mouse Genome Informatics
ht2
    Pdx1tm3Cvw/Pdx1+
involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
endocrine/exocrine glands
• islets contain higher numbers of pancreatic polypeptide (PP)-producing cells compared to wild-type mice
• islets contain higher numbers of glucagon-producing cells compared to wild-type mice
• glucagon-producing cells are often intermixed with beta cells, rather than being peripherally located as in wild-type

homeostasis/metabolism
• at 10 weeks of age, mice have higher glucose levels than wild-type
• levels are significantly decreased at 10 weeks
• at 8-11 weeks, plasma insulin is low 15 minutes after glucose challenge, with an overall smaller and delayed increase compared to controls
• at 8-11 weeks of age, mice have severely impaired glucose clearance compared to conrol littermates; levels are >600 mg/dl glucose in many animals


Mouse Genome Informatics
ht3
    Pdx1tm2Cvw/Pdx1tm3Cvw
involves: 129/Sv * C57BL/6 * DBA * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• most mice die by P9, and all are dead by P17

growth/size
• by 2 days postnatal, mice show growth retardation

endocrine/exocrine glands
• dorsal outgrowth found in place of pancreas lacks insulin, somatostatin, and PP, as well as exocrine markers like amylase
• pancreas is replaced by small rudiment protruding from duodenal wall; rudiment has glucagon-producing cells at tips of ductular tree that shows limited branching
• at E12, dorsal pancreatic bud is smaller with no evidence of branching morphogenesis compared to wild-type; no separate ventral pancreatic bud is present

digestive/alimentary system
• GIP-producing cells are decreased 63.4% compared to Pdxtm4.1Cvw homozygotes
• dorsal outgrowth found in place of pancreas lacks insulin, somatostatin, and PP, as well as exocrine markers like amylase

homeostasis/metabolism
• by 2 days postnatal, mice show dehydration