Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hsp90b1tm1Zhli mutation
(0 available);
any
Hsp90b1 mutation
(34 available)
Tg(Tyr-cre/ERT2)13Bos mutation
(12 available)
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pigmentation
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• mice show decreased levels of melanin in hair bulbs in the 4-OHT-treated area relative to control mice
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• mice show decreased levels of melanin in hair shafts plucked from the 4-OHT-treated area relative to control mice
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• following 4 weeks of topical application of 4-hydroxytamoxifen (4-OHT) to shaved skin on the right flank, coat hair that re-grows in the 4-OHT-treated area is grey/white in color, whereas hair re-grown in the vehicle (ethanol)-treated left flank is black; control mice treated with either 4-OHT or vehicle show no change in hair color
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integument
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• mice show decreased levels of melanin in hair bulbs in the 4-OHT-treated area relative to control mice
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• mice show decreased levels of melanin in hair shafts plucked from the 4-OHT-treated area relative to control mice
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• following 4 weeks of topical application of 4-hydroxytamoxifen (4-OHT) to shaved skin on the right flank, coat hair that re-grows in the 4-OHT-treated area is grey/white in color, whereas hair re-grown in the vehicle (ethanol)-treated left flank is black; control mice treated with either 4-OHT or vehicle show no change in hair color
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hsp90b1tm1.1Zhli mutation
(0 available);
any
Hsp90b1 mutation
(34 available)
Hsp90b1tm1Zhli mutation
(0 available);
any
Hsp90b1 mutation
(34 available)
Lyz2tm1(cre)Ifo mutation
(14 available);
any
Lyz2 mutation
(41 available)
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mortality/aging
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• mutants die from vascular collapse, intravascular coagulation and organ ischemia after gram-negative bacterial infection
• wild-type mice are more susceptible to LPS-induced sepsis than mutants, showing 63% mortality from endotoxemia compared to 17% mortality of mutants after 4 days
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immune system
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• macrophages isolated from mutants do not respond to ligands for TLR5 and TLR7
• less Il-6 or Il-12p40 in response to LPS or CpG olignucleotide stimulation, respectively, are secreted by mutant macrophages than by wild-type
• TNFalpha release is reduced in response to poly I:C stimulation compared to wild-type
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• 7-week old mice produce less cytokines like Il-6, Il-12p40 and TNFalpha than wild-type mice after LPS treatment
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• mutant show reduced response to LPS compared to wild-type
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• 3 days following infection by Listeria monocytogenes, mutants show significant weight loss and reduction of serum IL-12p40 and also show higher bacterial burden than wild-type
• 60-90% of spleen white pulp follicles are destroyed after 3 days with severe loss of lymphocytes
• numerous necrotic foci and abscesses are observed in infected mutant livers
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hematopoietic system
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• macrophages isolated from mutants do not respond to ligands for TLR5 and TLR7
• less Il-6 or Il-12p40 in response to LPS or CpG olignucleotide stimulation, respectively, are secreted by mutant macrophages than by wild-type
• TNFalpha release is reduced in response to poly I:C stimulation compared to wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hsp90b1tm1.1Zhli mutation
(0 available);
any
Hsp90b1 mutation
(34 available)
Hsp90b1tm1Zhli mutation
(0 available);
any
Hsp90b1 mutation
(34 available)
Tg(Ddx4-cre)1Dcas mutation
(2 available)
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reproductive system
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• adult males show normal testis weight-to-body weight ratio relative to control males
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• fluorescent HSPA5 (an ER chaperone) and TO-PRO-3 (DNA) staining revealed strong HSPA5 localization within the abnormal sperm head rather than at the level of the intermediate piece as observed in wild-type sperm
• streptavidin-FITC (biotinylated proteins in mitochondria) and TO-PRO-3 (DNA) staining revealed a stronger streptavidin-FITC labeling concentrated at the abnormal sperm head, unlike in wild-type sperm
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• all cauda epididymal sperm show head distortions including abnormal heads with (i) a rather elongated head, ii) a rounded-globular head, and iii) an enlarged-deformed head
• 86% of abnormal sperm heads include types (ii) and (iii)
• normal hook-shaped sperm heads are never observed
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• fluorescent Lens culinaris agglutinin (LCA)-fluorescein isothiocyanate (FITC) (glycoconjugates) and TO-PRO-3 (DNA) staining revealed a diffuse and variable labeling of the acrosome, unlike in wild-type sperm
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• ~40% of sperm heads are rounded-globular
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• ~40% of sperm heads are enlarged and deformed
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• cauda epididymal sperm are almost immobile
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• adult males are infertile
• however, mating behavior is normal
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cellular
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• fluorescent HSPA5 (an ER chaperone) and TO-PRO-3 (DNA) staining revealed strong HSPA5 localization within the abnormal sperm head rather than at the level of the intermediate piece as observed in wild-type sperm
• streptavidin-FITC (biotinylated proteins in mitochondria) and TO-PRO-3 (DNA) staining revealed a stronger streptavidin-FITC labeling concentrated at the abnormal sperm head, unlike in wild-type sperm
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• normal hook-shaped sperm heads are never observed
• all cauda epididymal sperm show head distortions including abnormal heads with (i) a rather elongated head, ii) a rounded-globular head, and iii) an enlarged-deformed head
• 86% of abnormal sperm heads include types (ii) and (iii)
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• fluorescent Lens culinaris agglutinin (LCA)-fluorescein isothiocyanate (FITC) (glycoconjugates) and TO-PRO-3 (DNA) staining revealed a diffuse and variable labeling of the acrosome, unlike in wild-type sperm
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• ~40% of sperm heads are rounded-globular
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• ~40% of sperm heads are enlarged and deformed
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• cauda epididymal sperm are almost immobile
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Analysis Tools