Analysis Tools
immune system
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• primary B cell follicles are absent and a ring-like B cell area typical of TNF-deficient mice is present
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• after immunization with sheep red blood cells (SRBCs), mutants fail to form organized germinal centers
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• after intraperitoneal injection of SRBC, mutants exhibit severely impaired SRBC-specific IgG1 antibody responses
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• levels of IgG1 specific for SRBC are lower than in wild-type
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• mutant mice exhibit physiologically relevant levels of biologically active transmembrane TNF protein in the complete absence of biologically active soluble TNF
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• mesenteric lymph nodes (MLN) and spleen of mutants lack follicular dendritic cells
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• mesenteric lymph nodes (MLN) lack organized B cell follicles but occasionally have GC-like regions that are centered in B cell areas
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• mesenteric lymph nodes lack organized B cell follicles and follicular dendritic cell networks
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• mutant mice exhibit physiologically relevant levels of biologically active transmembrane TNF protein in the complete absence of biologically active soluble TNF
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• mutant spleen cells isolated 49 days after myelin oligondendrocyte glycoprotein (MOG) showed no memory
• failed to proliferate in response to MOG peptide treatment
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• 25 days after injection with pertussis toxin, it is observed that experimental allergic encephalomyelitis (EAE) is completely suppressed compared to wild-type
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• when treated with D-gal (a hepatotoxin) at 20 mg/animal and doses of lipopolysaccharide (LPS) up to 100ug/25g body weight, mutants are completely resistant to LPS-induced death, but wild-type mice all die at 100-fold lower LPS doses
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• with challenge at high doses (10000 cfu) of Listeria monocytogenes (LM), mutants show high sensitivity with maximal lethality at 6 days post-infection, compared to wild-type; mutants show significant resistance when challenged with a physiological dose of LM (100 cfu)
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hematopoietic system
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• primary B cell follicles are absent and a ring-like B cell area typical of TNF-deficient mice is present
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• after immunization with sheep red blood cells (SRBCs), mutants fail to form organized germinal centers
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• after intraperitoneal injection of SRBC, mutants exhibit severely impaired SRBC-specific IgG1 antibody responses
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• levels of IgG1 specific for SRBC are lower than in wild-type
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homeostasis/metabolism
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• mutant mice exhibit physiologically relevant levels of biologically active transmembrane TNF protein in the complete absence of biologically active soluble TNF
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