Analysis Tools
mortality/aging
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• no pups present at P1, authors did not look at earlier stages
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Allele Symbol Allele Name Allele ID |
Kmt2atm1Clgr targeted mutation 1, Michael A Caligiuri MGI:3691062 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no pups present at P1, authors did not look at earlier stages
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Vertebral transformation is seen in Kmt2atm1Clgr/Mll1+ mice at 20 weeks of age
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• at E12.5 the boundary of Hoxa9 expression in the paraxial mesoderm is shifted ventrally
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• rudimentary or missing thirteenth rib in 70% of mice
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• rudimentary or missing thirteenth rib indicates transformation of T13 to L1
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• S1 duplication in 63% of mice, indicative of S2 to S1 transformation
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• increase in the number of mixed colony forming units (GEMM-CFU) formed by cultured splenocytes
• cell from splenocyte colony forming assays form larger secondary colonies in replating assays and are able to form tertiary and quaternary colonies unlike wild-type cells
however, in vivo cell counts in the bone marrow, spleen and blood are similar to wild type
• progenitor cells have a 2-fold increase in BrdU incorporation and a 50% increase in the amount of apoptosis
• progenitor cells are able to survive in culture for more than 4 months compared to less than 18 days for wild-type cells
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• increase in the number of myeloid colony forming units (GM-CFU) formed by cultured splenocytes
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• increase in the number of erythroid burst forming units (BFU-E) formed by cultured splenocytes
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• 5- to 20-fold increase in the number of Ter119+ erythroid cells in the spleen
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| N |
• despite changes in hematopoiesis and Hoxa gene expression mice do not develop leukemia
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• increase in the number of myeloid colony forming units (GM-CFU) formed by cultured splenocytes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• median survival of 49 weeks of age compared to 75-94 weeks of age for wild-type and single heterozygotes
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• 100% penetrance of acute leukemia at a median age of 49 weeks
• 70% develop acute myeloid leukemia
• 12% develop unclassifiable acute leukemia
• 9% develop B-cell leukemia
• 9% develop biphenotypic leukemia
• loss of Flt3 heterozygosity is seen in leukemias
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• presence of more than or equal to 20% blasts in blood and blasts in nonhematopoietic organs, including liver and adrenal glands
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• mutants develop leukocytosis
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• mutants develop leukocytosis
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| acute myeloid leukemia | DOID:9119 |
OMIM:601626 |
J:189096 | |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• median survival is 19 weeks of age
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• 89% develop acute myeloid leukemia
• 11% develop B-cell leukemia
• disease is more aggressive than in double heterozygous mutants
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| acute myeloid leukemia | DOID:9119 |
OMIM:601626 |
J:189096 | |
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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