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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Il5tm1Anjm
targeted mutation 1, Andrew NJ McKenzie
MGI:3688245
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Il5tm1Anjm/Il5tm1Anjm involves: 129P2/OlaHsd * C57BL/6 MGI:3689873
cx2
Il5tm1Anjm/Il5tm1Anjm
Il9tm1Anjm/Il9tm1Anjm
involves: 129P2/OlaHsd * C57BL/6 MGI:3689874


Genotype
MGI:3689873
hm1
Allelic
Composition
Il5tm1Anjm/Il5tm1Anjm
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il5tm1Anjm mutation (0 available); any Il5 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• goblet cell hyperplasia (increased number) is significantly delayed vs wild-type, and numbers do not increase until 30 days post-infection

immune system
• with absence of Il4 and Il5, eosinophilia is completely blocked
• in response to infection, mutants still generate mast cell hyperplasia (mast cell proliferation) equivalent to wild-type
• mutants produce higher levels of mast cell protease-1 compared to wild-type in response to parasitic infection
• mutants fail to produce IgE, due to deletion of Il4
• primary granuloma formation in and volumes in lungs after infection are severely reduced (<10%) compared to wild-type
• secondary granuloma formation is essentially completely blocked
• expulsion of worms from intestines (50% by day 35) is delayed compared to wild-type
• after N. brasiliensis (nematode) infection, expulsion of worms from intestines is delayed with ~50% of worms remaining in intestine after 25 days, whereas wild-type mice expel all worms within 5-10 days

digestive/alimentary system
• goblet cell hyperplasia (increased number) is significantly delayed vs wild-type, and numbers do not increase until 30 days post-infection

hematopoietic system
• with absence of Il4 and Il5, eosinophilia is completely blocked
• in response to infection, mutants still generate mast cell hyperplasia (mast cell proliferation) equivalent to wild-type
• mutants produce higher levels of mast cell protease-1 compared to wild-type in response to parasitic infection
• mutants fail to produce IgE, due to deletion of Il4




Genotype
MGI:3689874
cx2
Allelic
Composition
Il5tm1Anjm/Il5tm1Anjm
Il9tm1Anjm/Il9tm1Anjm
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il5tm1Anjm mutation (0 available); any Il5 mutation (23 available)
Il9tm1Anjm mutation (2 available); any Il9 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection

immune system
• with absence of Il4 and Il5, eosinophilia is completely blocked
• mastocytosis is abolished compared to wild-type or triple mutants
• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations
• Il-10 expression is reduced compared to Il5tm1Anjm mutants
• mice show a switch to a Th1 response and Il12 production from a Th2 response
• mutants fail to produce IgE, due to deletion of Il4
• primary granuloma formation and volumes in lungs after infection are severely reduced (<10%) compared to wild-type
• secondary granuloma formation is essentially blocked
• expulsion of worms is delayed by an order of magnitude vs triple mutants
• compound quadruple mutants show ~1 order of magnitude greater delay in expulsion of worms from intestine (50% remain at day 60 after infection) than triple mutants (35 days)
• more worms remain at 40 days post infection than in triple Il4/Il5/Il13 mutants or wild-type

digestive/alimentary system
• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection

hematopoietic system
• with absence of Il4 and Il5, eosinophilia is completely blocked
• mastocytosis is abolished compared to wild-type or triple mutants
• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations
• mutants fail to produce IgE, due to deletion of Il4

homeostasis/metabolism
• Il-10 expression is reduced compared to Il5tm1Anjm mutants





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/14/2020
MGI 6.14
The Jackson Laboratory