About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Igktm1Rsky
targeted mutation 1, Klaus Rajewsky
MGI:3662618
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Ightm2Rsky/Igh+
Igktm1Rsky/Igk+ 		either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c) MGI:3689731
cx2
 		Ightm2Cgn/Igh+
Igktm1Rsky/Igk+ 		either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c) MGI:3689733
cx3
 		Blnktm1Kplm/Blnktm1Kplm
Ightm4Cgn/Igh+
Igktm1Rsky/Igk+ 		involves: 129P2/OlaHsd MGI:3844072
cx4
 		Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Trp53tm1Tyj/Trp53tm1Tyj
Xrcc4tm1Fwa/Xrcc4tm1Fwa 		involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac MGI:4437907
cx5
 		Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Tyj/Trp53tm1Tyj 		involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac MGI:4437910
cx6
 		Igktm1Rsky/Igk+
Igll1tm1Cgn/Igll1tm1Cgn 		involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6 MGI:3689735


Genotype
MGI:3689731
cx1
Allelic
Composition		 Ightm2Rsky/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Rsky mutation (2 available); any Igh mutation (43 available)
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:111431 )
• lack 3-83Ig+ mature B cells, although on an H2d background some 3-83Ig+ immature B cells are detected in the bone marrow
• about and 8-fold and 15-fold increase in the frequency and absolute number of lambda expressing splenic B cells, respectively, compared to wild-type mice
• however, total B cell numbers are similar to wild-type

hematopoietic system
abnormal B cell differentiation ( J:111431 )
• lack 3-83Ig+ mature B cells, although on an H2d background some 3-83Ig+ immature B cells are detected in the bone marrow
• about and 8-fold and 15-fold increase in the frequency and absolute number of lambda expressing splenic B cells, respectively, compared to wild-type mice
• however, total B cell numbers are similar to wild-type




Genotype
MGI:3689733
cx2
Allelic
Composition		 Ightm2Cgn/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (43 available)
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:111431 )
• about 90% of B cells are positive for the B1-8 heavy chain
absent pre-B cells ( J:111431 )
• the CD43- sIgM- pre B cell compartment is virtually absent

hematopoietic system
abnormal B cell differentiation ( J:111431 )
• about 90% of B cells are positive for the B1-8 heavy chain
absent pre-B cells ( J:111431 )
• the CD43- sIgM- pre B cell compartment is virtually absent




Genotype
MGI:3844072
cx3
Allelic
Composition		 Blnktm1Kplm/Blnktm1Kplm
Ightm4Cgn/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Blnktm1Kplm mutation (0 available); any Blnk mutation (76 available)
Ightm4Cgn mutation (0 available); any Igh mutation (43 available)
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:120460 )
• innocuous transgenic BCR on Blnk null B cells leads to enhanced development of these B cells compared to Blnk homozygotes
• two populations are detected in the bone marrow consisting of IgMlo and IgMhi groups
• both populations appear to be immature B cells as they have low surface level expression of MHC-II and CD43, and high levels of CD24
• based on intensity of these surface markers, the IgMlo population is the less mature of the two
• in vitro maturation of bone marrow cells demonstrate delayed development of these IgM+ cells
• B cells in the periphery are primarily T1 stage B cells that have a short half-life
increased transitional stage B cell number ( J:120460 )
• B cells in the periphery are primarily T1 stage B cells
decreased B cell number ( J:120460 )
• B cell numbers are decreased 2- to 3- fold in the spleen
• the B cells present are primarily T1 stage B cells
increased pre-B cell number ( J:120460 )
• pre-B cell numbers are 2-3 fold higher in the bone marrow than in transgenic controls

hematopoietic system
abnormal B cell differentiation ( J:120460 )
• innocuous transgenic BCR on Blnk null B cells leads to enhanced development of these B cells compared to Blnk homozygotes
• two populations are detected in the bone marrow consisting of IgMlo and IgMhi groups
• both populations appear to be immature B cells as they have low surface level expression of MHC-II and CD43, and high levels of CD24
• based on intensity of these surface markers, the IgMlo population is the less mature of the two
• in vitro maturation of bone marrow cells demonstrate delayed development of these IgM+ cells
• B cells in the periphery are primarily T1 stage B cells that have a short half-life
increased transitional stage B cell number ( J:120460 )
• B cells in the periphery are primarily T1 stage B cells
decreased B cell number ( J:120460 )
• B cell numbers are decreased 2- to 3- fold in the spleen
• the B cells present are primarily T1 stage B cells
increased pre-B cell number ( J:120460 )
• pre-B cell numbers are 2-3 fold higher in the bone marrow than in transgenic controls




Genotype
MGI:4437907
cx4
Allelic
Composition		 Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Trp53tm1Tyj/Trp53tm1Tyj
Xrcc4tm1Fwa/Xrcc4tm1Fwa
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (43 available)
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (232 available)
Xrcc4tm1Fwa mutation (0 available); any Xrcc4 mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 is 20% to 50% of wild-type
• class switching to IgG3 is 50% of wild-type
• IgH class switching is reduced compared to in wild-type B cells

cellular
spontaneous chromosome breakage ( J:126758 )
• B cell stimulated with anti-CD40 and IL4 exhibit metaphase chromosome breaks unlike similarly treated wild-type cells

hematopoietic system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 is 20% to 50% of wild-type
• class switching to IgG3 is 50% of wild-type
• IgH class switching is reduced compared to in wild-type B cells




Genotype
MGI:4437910
cx5
Allelic
Composition		 Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Tyj/Trp53tm1Tyj
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (43 available)
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
Lig4tm1Fwa mutation (1 available); any Lig4 mutation (44 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (232 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 in B cells stimulated with anti-CD40 plus IL4 are 50% of wild-type

immune system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 in B cells stimulated with anti-CD40 plus IL4 are 50% of wild-type




Genotype
MGI:3689735
cx6
Allelic
Composition		 Igktm1Rsky/Igk+
Igll1tm1Cgn/Igll1tm1Cgn
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igktm1Rsky mutation (1 available); any Igk mutation (25 available)
Igll1tm1Cgn mutation (1 available); any Igll1 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:35357 )
• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain
decreased immature B cell number ( J:35357 )
• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased B cell number ( J:35357 )
• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice
decreased mature B cell number ( J:35357 )
• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased pre-B cell number ( J:35357 )
• about a 3-fold reduction compared to wild-type mice

hematopoietic system
abnormal B cell differentiation ( J:35357 )
• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain
decreased immature B cell number ( J:35357 )
• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased B cell number ( J:35357 )
• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice
decreased mature B cell number ( J:35357 )
• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased pre-B cell number ( J:35357 )
• about a 3-fold reduction compared to wild-type mice




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory