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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(tetO-Ipf1,EGFP)956.6Macd
transgene insertion 956-6, Raymond J MacDonald
MGI:3655514
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Pdx1tm1Macd/Pdx1tm1Macd
Tg(tetO-Ipf1,EGFP)956.6Macd/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3655708


Genotype
MGI:3655708
cx1
Allelic
Composition
Pdx1tm1Macd/Pdx1tm1Macd
Tg(tetO-Ipf1,EGFP)956.6Macd/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pdx1tm1Macd mutation (1 available); any Pdx1 mutation (28 available)
Tg(tetO-Ipf1,EGFP)956.6Macd mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• in doxycycline-treated mice there is an increase in glucagons-positive cells
• there is a marked reduction in insulin in pancreata of doxycycline-treated mice; after withdrawal of doxycycline, insulin +ve cells are detected in islets of smaller islet-like cell clusters
• 14 days after doxycycline withdrawal after 14 days of treatment resulted in a significant decrease in blood glucose levels with 4/5 mice becoming normoglycemic by 28 days
• with doxycycline treatment for 21 day, adult rescue mice show a 4-fold increase in fasting blood glucose to diabetic levels
• adult transgenic rescue animals treated with doxycycline for 14 days exhibit a defective response to glucose challenge compared to wild-type, non mutant transgenic, or untreated rescue mice (J:79206)
• when adult mice are treated with doxycycline for 0, 7, 14, or 21 days, mice show an increased early glucose response within 7 days of start of treatment and ability to recover from glucose challenge is impaired compared to untreated transgenics where glucose levels recover to basal levels within 3 hours; by 14 days of doxycycline treatment, mice have diabetes (J:101742)

endocrine/exocrine glands
• after 14 or 28 days of dox treatment there are, still present, beta cells which lack insulin
• after dox treatment for 14 days, there is a significant decrease in islet area compared to wild-type
• pancreas of transgenic rescue newborn mice is 50% the size of a normal wild-type neonatal pancreas
• treatment of pregnant mice with doxycycline from the first day of pregnancy through parturition prevents formation of the pancreas in mice with the transgenic rescue genotype
• treatment on E11.5 arrests pancreatic development ~36 hours later; at birth the underdeveloped remnant consisted of a large and extended duct with several terminal, aborted, ductal buds with ducts lined with a single layer of primitive epithelial cells and with no acinar or islet tissue
• in doxycycline-treated mice there is an increase in glucagons-positive cells
• there is a marked reduction in insulin in pancreata of doxycycline-treated mice; after withdrawal of doxycycline, insulin +ve cells are detected in islets of smaller islet-like cell clusters

cellular
• foci of duct proliferation are present in mice after 14 days of doxycycline treatment and become more prominent with continued treatment





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory