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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Fgf8tm2Moon
targeted mutation 2, Anne M Moon
MGI:3639320
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Fgf8tm2Moon/Fgf8tm1Mrc
Isl1tm1(cre)Sev/Isl1+ 		involves: 129S/Sv MGI:3639731
cn2
 		Fgf8tm1Mrc/Fgf8tm2Moon
Isl1tm1(cre)Sev/Isl1+ 		involves: 129S/Sv * Black Swiss * C57BL/6 MGI:3829040
cn3
 		Fgf8tm2Moon/Fgf8tm1Mrc
Mesp1tm2(cre)Ysa/Mesp1+ 		involves: C57BL/6 * CBA MGI:3639730
cn4
 		Fgf8tm1Mrc/Fgf8tm2Moon
Foxa2tm2.1(cre/Esr1*)Moon/Foxa2+ 		Not Specified MGI:3829041
cn5
 		Fgf8tm2Moon/Fgf8tm1Mrc
Tg(Mef2c-cre)2Blk/0 		Not Specified MGI:3639738


Genotype
MGI:3639731
cn1
Allelic
Composition		 Fgf8tm2Moon/Fgf8tm1Mrc
Isl1tm1(cre)Sev/Isl1+
Genetic
Background		 involves: 129S/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fgf8tm1Mrc mutation (0 available); any Fgf8 mutation (18 available)
Fgf8tm2Moon mutation (0 available); any Fgf8 mutation (18 available)
Isl1tm1(cre)Sev mutation (1 available); any Isl1 mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, incomplete penetrance ( J:109475 )
• severely affected mutants (35%) die by E10; most (65%) survive to birth

embryo
small pharyngeal arch ( J:109475 )
• at E8.75 -10.5, surviving mutants have small pharyngeal arches

cellular
increased apoptosis ( J:109475 )
• excess apoptotic cells are detected at the 7-9 somite stage in ventral endoderm and adjacent smooth muscle

cardiovascular system
abnormal truncus arteriosus septation ( J:109475 )
• conotruncal cushions are hypocellular compared to controls; fusion of cushions to form AP septum is delayed in mutants
persistent truncus arteriosus ( J:109475 )
• 100% of E18.5/newborns have PTA
abnormal heart right ventricle morphology ( J:109475 )
• at E8.75 -10.5, surviving mutants have severe right ventricle hypoplasia

craniofacial
small pharyngeal arch ( J:109475 )
• at E8.75 -10.5, surviving mutants have small pharyngeal arches




Genotype
MGI:3829040
cn2
Allelic
Composition		 Fgf8tm1Mrc/Fgf8tm2Moon
Isl1tm1(cre)Sev/Isl1+
Genetic
Background		 involves: 129S/Sv * Black Swiss * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fgf8tm1Mrc mutation (0 available); any Fgf8 mutation (18 available)
Fgf8tm2Moon mutation (0 available); any Fgf8 mutation (18 available)
Isl1tm1(cre)Sev mutation (1 available); any Isl1 mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
abnormal cardiac epithelial to mesenchymal transition ( J:143444 )
• in explants no cells manage to successfully undergo endothelial to mesenchymal transformation
abnormal cardiac outflow tract development ( J:143444 )
• outflow tracts are short and abnormally angulated
abnormal conotruncal ridge morphology ( J:143444 )
• outflow tract cushions contain less cardiac jelly and fewer mesenchymal cells
• proximal outflow tract cushions are thinner and contain fewer cells while distal cushions are thinner but do not display a change in cell density
persistent truncus arteriosus ( J:143444 )
• all show type III PTA (the outflow tract is unseptated along its entire proximodstal extent)




Genotype
MGI:3639730
cn3
Allelic
Composition		 Fgf8tm2Moon/Fgf8tm1Mrc
Mesp1tm2(cre)Ysa/Mesp1+
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fgf8tm1Mrc mutation (0 available); any Fgf8 mutation (18 available)
Fgf8tm2Moon mutation (0 available); any Fgf8 mutation (18 available)
Mesp1tm2(cre)Ysa mutation (1 available); any Mesp1 mutation (17 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, incomplete penetrance ( J:109475 )
• severely affected Fgf8-deficient embryos (65%) die by E10

homeostasis/metabolism
pericardial effusion ( J:109475 )
• embryos die by E10 with pericardial effusion in 65% of embryos
edema ( J:109475 )
• embryos die by E10 with generalized edema in 65% of embryos

cardiovascular system
abnormal cardiac outflow tract development ( J:109475 )
• OFTs are short or absent in 65% of embryos
abnormal heart tube morphology ( J:109475 )
• heart tube is hypoplastic in 65% of embryos
transposition of great arteries ( J:109475 )
• 30% of the embryos born show transposition of the great arteries
common atrium ( J:109475 )
• there is a single dilated atrium in 65% of embryos
common ventricle ( J:109475 )
• there is a single dilated ventricle in 65% of embryos
bicuspid aortic valve ( J:109475 )
• 40% of newborns also have a bicuspid aortic valve
bicuspid pulmonary valve ( J:109475 )
• 40% of newborns also have a bicuspid pulmonary valve
decreased heart right ventricle size ( J:109475 )
• 35% of embryos survive but have small right ventricles at midgestation
pericardial effusion ( J:109475 )
• embryos die by E10 with pericardial effusion in 65% of embryos

cellular
increased apoptosis ( J:109475 )
• excess apoptotic cells are detected at the 7-9 somite stage in ventral endoderm and adjacent smooth muscle
decreased cell proliferation ( J:109475 )
• at the 4 somite stage, mutants have 46% fewer proliferating cells in crescent mesoderm; this persisted to the 9 somite stage
• proliferating cells are decreased in the proximal outflow tract and pharyngeal epithelium at the 9 somite stage




Genotype
MGI:3829041
cn4
Allelic
Composition		 Fgf8tm1Mrc/Fgf8tm2Moon
Foxa2tm2.1(cre/Esr1*)Moon/Foxa2+
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fgf8tm1Mrc mutation (0 available); any Fgf8 mutation (18 available)
Fgf8tm2Moon mutation (0 available); any Fgf8 mutation (18 available)
Foxa2tm2.1(cre/Esr1*)Moon mutation (1 available); any Foxa2 mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:143444 )
N
• no defects in outflow tract development are seen




Genotype
MGI:3639738
cn5
Allelic
Composition		 Fgf8tm2Moon/Fgf8tm1Mrc
Tg(Mef2c-cre)2Blk/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fgf8tm1Mrc mutation (0 available); any Fgf8 mutation (18 available)
Fgf8tm2Moon mutation (0 available); any Fgf8 mutation (18 available)
Tg(Mef2c-cre)2Blk mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
double outlet right ventricle ( J:109475 )
• 25% of mutants have TGA and double outlet right ventricle
transposition of great arteries ( J:109475 )
• 25% of mutants have TGA and double outlet right ventricle




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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory