Phenotypes associated with this allele

• Allele Symbol: Ccr5^tm1Blck
• Allele Name: targeted mutation 1, Bruno Luckow
• Allele ID: MGI:3613371
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccr5^tm1Blck/Ccr5^tm1Blck	B6.129P2-Ccr5^tm1Blck	MGI:3614447
cx2	Apoe^tm1Unc/Apoe^tm1Unc Ccr5^tm1Blck/Ccr5^tm1Blck	involves: 129P2/OlaHsd * C57BL/6	MGI:5317846

Genotype
MGI:3614447

hm1

• Allelic Composition: Ccr5^tm1Blck/Ccr5^tm1Blck
• Genetic Background: B6.129P2-Ccr5^tm1Blck

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal response to cardiac infarction ( J:104744 )

• recruitment of neutrophils during reperfusion is reduced, with reduced leukocyte adhesion to the inner vessel wall of postcapillary venules and reduced number of transmigrated leukocytes

immune system

abnormal cellular extravasation ( J:104744 )

• at 120 min of reperfusion, show a 48.5% reduction in the number of transmigrated leukocytes compared to wild-type, however leukocyte extravascular migration distances are comparable to wild-type

abnormal leukocyte adhesion ( J:104744 )

• at 5 min of reperfusion, significantly fewer leukocytes adhere to the inner vessel wall of postcapillary venules than in wild-type, however no differences are seen with respect to then number of rolling leukocytes

increased length of allograft survival ( J:105072 )

• survival of transplated cardiac allographs from BALB/c mice into homozygotes is significantly increased and allografts show less tissue remodeling and hence better preservation of myocardial architecture

• carotid artery allografts from BALB/c mice into homozygotes show better tissue preservation and significant reduction of neointima formation and CD3+ T cell infiltration

homeostasis/metabolism

abnormal response to cardiac infarction ( J:104744 )

• recruitment of neutrophils during reperfusion is reduced, with reduced leukocyte adhesion to the inner vessel wall of postcapillary venules and reduced number of transmigrated leukocytes

cellular

abnormal cellular extravasation ( J:104744 )

• at 120 min of reperfusion, show a 48.5% reduction in the number of transmigrated leukocytes compared to wild-type, however leukocyte extravascular migration distances are comparable to wild-type

abnormal leukocyte adhesion ( J:104744 )

• at 5 min of reperfusion, significantly fewer leukocytes adhere to the inner vessel wall of postcapillary venules than in wild-type, however no differences are seen with respect to then number of rolling leukocytes

hematopoietic system

abnormal cellular extravasation ( J:104744 )

• at 120 min of reperfusion, show a 48.5% reduction in the number of transmigrated leukocytes compared to wild-type, however leukocyte extravascular migration distances are comparable to wild-type

abnormal leukocyte adhesion ( J:104744 )

• at 5 min of reperfusion, significantly fewer leukocytes adhere to the inner vessel wall of postcapillary venules than in wild-type, however no differences are seen with respect to then number of rolling leukocytes

Genotype
MGI:5317846

cx2

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Ccr5^tm1Blck/Ccr5^tm1Blck
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cardiovascular system

atherosclerotic lesions ( J:128063 )

• reduced plaque areas after 10-12 weeks on a high fat or high cholesterol diet

• both in the aortic root and the thoracoabdominal artery

• also at 22 weeks when fed a normal diet

immune system

decreased T cell number ( J:128063 )

• in atherosclerotic plaques reduced 24% compared to Apoe^tm1Unc

abnormal mononuclear phagocyte morphology ( J:128063 )

• monocyte/macrophage in atherosclerotic plaques reduced 77% compared to Apoe^tm1Unc

abnormal lymphocyte physiology ( J:128063 )

• reduced proliferative responses of splenocytes and of lymph node cells by 25% compared to Apoe^tm1Unc

abnormal cytokine secretion ( J:128063 )

decreased interferon-gamma secretion ( J:128063 )

• significantly decreased secretion by splenocytes

increased interleukin-10 secretion ( J:128063 )

• increased content in atherosclerotic plaques

• increased secretion by both splenocytes and by lymph node cells

hematopoietic system

decreased T cell number ( J:128063 )

• in atherosclerotic plaques reduced 24% compared to Apoe^tm1Unc

abnormal mononuclear phagocyte morphology ( J:128063 )

• monocyte/macrophage in atherosclerotic plaques reduced 77% compared to Apoe^tm1Unc

abnormal lymphocyte physiology ( J:128063 )

• reduced proliferative responses of splenocytes and of lymph node cells by 25% compared to Apoe^tm1Unc