About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mus81tm1Chmg
targeted mutation 1, Clare H McGowan
MGI:3588581
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mus81tm1Chmg/Mus81tm1Chmg involves: 129S4/SvJae * 129S5/SvEvBrd * C57BL/6 MGI:3589458
hm2
Mus81tm1Chmg/Mus81tm1Chmg involves: 129S5/SvEvBrd MGI:3794047
hm3
Mus81tm1Chmg/Mus81tm1Chmg involves: 129S5/SvEvBrd * C57BL/6 MGI:5559493
cx4
Mus81tm1Chmg/Mus81tm1Chmg
Slx1btm1.1Jrou/Slx1btm1.1Jrou
involves: 129P2/OlaHsd * 129S5/SvEvBrd * C57BL/6 MGI:5559494
cx5
Fan1tm1d(KOMP)Wtsi/Fan1tm1d(KOMP)Wtsi
Mus81tm1Chmg/Mus81tm1Chmg
involves: 129S5/SvEvBrd * C57BL/6N * FVB/N MGI:5897867


Genotype
MGI:3589458
hm1
Allelic
Composition
Mus81tm1Chmg/Mus81tm1Chmg
Genetic
Background
involves: 129S4/SvJae * 129S5/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mus81tm1Chmg mutation (0 available); any Mus81 mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes display increased sensitivity to mitomycin C-induced lethality but did not display premature aging, decreased life spans, or increased tumor incidence

cellular
• homozygous MEFs die after 3 - 5 days in culture with a significant decrease in cells with 4N DNA and increase in cells with sub-G1 content of DNA after 2 days in culture; however, homozygous mice are viable and display normal growth, immune function, and fertility
• retroviral expression of the viral protein E6 which promotes degradation of Trp53 allows homozygous MEFs to survive in culture although these cells display increased DNA damage and chromosomal aberrations

homeostasis/metabolism
• homozygotes display increased sensitivity to mitomycin C-induced lethality but did not display premature aging, decreased life spans, or increased tumor incidence




Genotype
MGI:3794047
hm2
Allelic
Composition
Mus81tm1Chmg/Mus81tm1Chmg
Genetic
Background
involves: 129S5/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mus81tm1Chmg mutation (0 available); any Mus81 mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• unlike mice homozygous for Mus81tm1Esse, no defects are seen in the aortic wall including the elastin layers

homeostasis/metabolism
• MEFs are more sensitive to mitomycin C compared to wild-type cells but less sensitive than MEFs null for Fan1




Genotype
MGI:5559493
hm3
Allelic
Composition
Mus81tm1Chmg/Mus81tm1Chmg
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mus81tm1Chmg mutation (0 available); any Mus81 mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• in mouse embryonic fibroblasts treated with DNA interstrand crosslinking agents (MMC)
• however, MMC-induced increase in sister chromatid exchange frequency is normal in mouse embryonic fibroblasts
• Blm-depleted mouse embryonic fibroblasts exhibit reduced sister chromatid exchange frequency compared with wild-type cells

homeostasis/metabolism
• Blm-depleted mouse embryonic fibroblasts exhibit reduced sister chromatid exchange frequency compared with wild-type cells




Genotype
MGI:5559494
cx4
Allelic
Composition
Mus81tm1Chmg/Mus81tm1Chmg
Slx1btm1.1Jrou/Slx1btm1.1Jrou
Genetic
Background
involves: 129P2/OlaHsd * 129S5/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mus81tm1Chmg mutation (0 available); any Mus81 mutation (29 available)
Slx1btm1.1Jrou mutation (0 available); any Slx1b mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• mice exhibit normal fertility and testes size

cellular
• in mouse embryonic fibroblasts treated with DNA interstrand crosslinking agents (MMC) to the same extent as in single homozygotes
• however, MMC-induced increase in sister chromatid exchange frequency is normal in mouse embryonic fibroblasts
• Blm-depleted mouse embryonic fibroblasts exhibit reduced sister chromatid exchange frequency as in single homozygotes

homeostasis/metabolism
• Blm-depleted mouse embryonic fibroblasts exhibit reduced sister chromatid exchange frequency as in single homozygotes




Genotype
MGI:5897867
cx5
Allelic
Composition
Fan1tm1d(KOMP)Wtsi/Fan1tm1d(KOMP)Wtsi
Mus81tm1Chmg/Mus81tm1Chmg
Genetic
Background
involves: 129S5/SvEvBrd * C57BL/6N * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fan1tm1d(KOMP)Wtsi mutation (0 available); any Fan1 mutation (98 available)
Mus81tm1Chmg mutation (0 available); any Mus81 mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• MEFs are as sensitive to mitomycin C as cells from mice homozygous for Fan1 alone





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/12/2024
MGI 6.23
The Jackson Laboratory