Mouse Genome Informatics
hm1
    Rbpjtm1Hon/Rbpjtm1Hon
involves: 129P2/OlaHsd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
N
• normal B cell development (J:76240)


Mouse Genome Informatics
cn2
    Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Rbpjtm1Hon/Rbpjtm1Hon
Tg(tetO-cre)1Jaw/0

involves: 129 * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• doxycycline-treated mice die shortly after birth

respiratory system
• at E18.5, myofibroblasts in the lungs of doxycycline-treated mice exhibit reduced differentiation, as determined by sma+ expression, compared to in wild-type mice


Mouse Genome Informatics
cn3
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Lbx1-cre)3Cbm/0

involves: 129P2/OlaHsd
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• expected Mendelian ratios of mice are born but homozygotes fail to suckle and subsequently die within the first postnatal day

muscle
• at E11.5, more progenitor cells have started to differentiate (indicated by increased Myod expression)
• at E12.5 in the limbs, more cells express markers of myogenic differentiation and fewer progenitor cells remain
• at E14.5 in the limbs, fewer muscle progenitor cells are present and those present have reduced proliferative capacity
• at E18.5 all cells are postmitotic unlike in wild-type
• however, the muscle progenitor cells migration is unaffected
• at E14.5 there is a reduction in the size of limb muscle groups
• absence of satellite cells in limb muscle at E18.5
• very few satellite cells are seen in intrinsic tongue muscle at E14.5 however, satellite cells can be detected in the distal forelimb
• muscle fibers in limb muscle have reduced density and the ratio of Myod+ nuclei/fiber is reduced
• however, myofiber diameter is comparable to wild-type

behavior/neurological
• homozygotes fail to suckle


Mouse Genome Informatics
cn4
    Pax3tm1(cre)Joe/Pax3+
Rbpjtm1Hon/Rbpjtm1Hon

involves: 129P2/OlaHsd
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• expected Mendelian ratios are born but homozygous mice do not move or breath and die shortly after birth

muscle
• at E11.5, progenitor cell numbers in the myotome are decreased and the number of differentiated cells is increased compared to wild-type
• at E14.5 residual back muscles are small and lack progenitor cells
• limb muscles have reduced myogenic precursor cells and lack satellite cells
• diaphragm is smaller
• intercostal muscles are small
• at E14.5, the size of limb muscle groups is reduced
• absence of satellite cells in limb, intercostals, diaphragm and deep back muscles

respiratory system

behavior/neurological
• no movement at birth

nervous system
• at E10.5 and E11.5, mice exhibit precocious neuronal differentiation compared to in wild-type mice
• overall neurogenesis is increased in the dorsal spinal cord
• the numbers of dI2 and dI3 neurons are increased slightly while the number of dI5 neurons is increased dramatically compared to in wild-type mice
• the progenitor domain is reduced compared to in wild-type mice
• the dorsal neural tube in E10.5 embyros lacks dI4 neurons with the number of dI2, dI3, and dI5 neurons being increased
• the number of dI5 neurons is dramatically increased while increases in dI2 and dI3 neurons are more modest
• there is a significant decrease in the thickness of the progenitor domain of the E11.5 neural tube and a corresponding increase in the neuronal domain
• the dorsal progenitor domain of the neural tube is depleted by E12
• mice exhibit a reduction in the number of dI4 neurons compared to in wild-type mice
• the numbers of dI3 neurons are increased slightly compared to in wild-type mice
• mice exhibit a complete loss of dI4 interneurons

embryogenesis
• the dorsal neural tube in E10.5 embyros lacks dI4 neurons with the number of dI2, dI3, and dI5 neurons being increased
• the number of dI5 neurons is dramatically increased while increases in dI2 and dI3 neurons are more modest
• there is a significant decrease in the thickness of the progenitor domain of the E11.5 neural tube and a corresponding increase in the neuronal domain
• the dorsal progenitor domain of the neural tube is depleted by E12

cellular
• at E10.5 and E11.5, mice exhibit precocious neuronal differentiation compared to in wild-type mice
• overall neurogenesis is increased in the dorsal spinal cord
• the numbers of dI2 and dI3 neurons are increased slightly while the number of dI5 neurons is increased dramatically compared to in wild-type mice
• the progenitor domain is reduced compared to in wild-type mice


Mouse Genome Informatics
cn5
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Pax2-cre)1Akg/0

involves: 129P2/OlaHsd
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• embryos are normal at E12.5, but die at E13.5

renal/urinary system
• podocytes with WT1high expression do not develop in E12.5 cultures
• cultured E12.5 mtanephroi branch normally, but tubules positive for LTL (lectin) are not detected


Mouse Genome Informatics
cn6
    Rbpjtm1Hon/Rbpjtm1.1Hon
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Ptf1atm2(cre/ESR1)Cvw/Ptf1a+

involves: 129P2/OlaHsd * 129S1/Sv * 129S6/SvEvTac
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
endocrine/exocrine glands
N
• tamoxifen-treated mice exhibit normal proliferation of YFP+ acinar cells (J:180310)


Mouse Genome Informatics
cn7
    Rbpjtm1Hon/Rbpjtm1.1Hon
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+
Hes1tm1(cre/ERT2)Lcm/Hes1+

involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
endocrine/exocrine glands
N
• tamoxifen-treated mice exhibit normal pancreatic ductal morphology and pancreata mass (J:180310)
• tamoxifen-treated mice exhibit an increase in YFP+ acinar cells compared with control mice
• tamoxifen-treated mice exhibit a decrease in YFP+ centroacinar cells compared with control mice
• tamoxifen-treated mice exhibit a rapid transformation of YFP+ centroacinar cells into acinar cells compared with control mice
• however, tamoxifen-treated mice exhibit normal centroacinar and acinar cells proliferation and apoptosis

digestive/alimentary system
• tamoxifen-treated mice exhibit an increase in YFP+ acinar cells compared with control mice
• tamoxifen-treated mice exhibit a decrease in YFP+ centroacinar cells compared with control mice


Mouse Genome Informatics
cn8
    Rbpjtm1Hon/Rbpjtm1.1Hon
Shhtm2(cre/ERT2)Cjt/Shh+

involves: 129P2/OlaHsd * 129S6/SvEvTac
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
respiratory system
• at E18.5, the respiratory epithelium was populated almost exclusively by Foxj1-expressing ciliated cells, unlike in control lungs where ciliated cells were interspersed with secretory Clara cells
• at E18.5, Ki67 labeling was dramatically reduced in large, medium and small airways, suggesting a severe decrease in epithelial cell proliferation relative to control lungs
• however, goblet cell fate was not lost, as shown by PAS and Alcian Blue staining of tracheal sections at birth (P0)
• no secretory Clara cells were detected at E18.5, as determined by specific marker analysis


Mouse Genome Informatics
cn9
    Rbpjtm1Hon/Rbpjtm1.1Hon
Tg(Pax7-cre/ERT2)1Cbm/0

involves: 129P2/OlaHsd * BALB/cJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
nervous system
• following treatment with tamoxifen, mice exhibit a reduction in the number of dILA neurons and an increased in the number of dILB neurons compared to in wild-type mice
• at E13.5 following treatment with tamoxifen, the progenitor domain is reduced compared to in wild-type mice
• there is a decrease in the thickness of the progenitor zone in the neural tube of E13.5 embryos when tamoxifen is given at E10.5
• tamoxifen induction at E10.5 leads to about a 50% reduction in dILA neurons in newborns and about a 50% increase in dILB neurons
• following treatment with tamoxifen, mice exhibit a reduction in the number of dILA neurons compared to in wild-type mice
• following treatment with tamoxifen, mice exhibit an increased in the number of dILB neurons compared to in wild-type mice

embryogenesis
• there is a decrease in the thickness of the progenitor zone in the neural tube of E13.5 embryos when tamoxifen is given at E10.5
• tamoxifen induction at E10.5 leads to about a 50% reduction in dILA neurons in newborns and about a 50% increase in dILB neurons

cellular
• following treatment with tamoxifen, mice exhibit a reduction in the number of dILA neurons and an increased in the number of dILB neurons compared to in wild-type mice
• at E13.5 following treatment with tamoxifen, the progenitor domain is reduced compared to in wild-type mice


Mouse Genome Informatics
cn10
    Rbpjtm1Kyo/Rbpjtm1Hon
Tg(Tek-cre)12Flv/0

involves: 129P2/OlaHsd * C3H * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
cardiovascular system
• the diameter of the dorsal aorta is reduced at E9.5
• at E9.5 PECAM-1 staining revealed a complete lack of vascular remodeling
• the distal outflow tract is connected to the anterior cardinal vein by an anastamoses
• some embryos also have more caudal fusions of the dorsal aorta to the common cardinal vein
• an avascular yolk is seen at E9.5
• pericardial effusion is seen at E9.5

embryogenesis
• an avascular yolk is seen at E9.5
• embryonic growth retardation is seen at E9.5

growth/size
• embryonic growth retardation is seen at E9.5

homeostasis/metabolism
• pericardial effusion is seen at E9.5


Mouse Genome Informatics
cn11
    Mesp1tm2(cre)Ysa/Mesp1+
Rbpjtm1Hon/Rbpjtm1Hon
Tg(CAG-cat,-Notch1)1Ysa/0

involves: 129P2/OlaHsd * C3H * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
cardiovascular system
• defects in heart morphology and development are unaffected by the presence of the transgene
• defects in heart morphology and development are unaffected by the presence of the transgene


Mouse Genome Informatics
cn12
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Vav1-cre)A2Kio/0

involves: 129P2/OlaHsd * C57BL/10 * CBA/Ca
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
tumorigenesis
• transplanted bone marrow progenitors transfected with BCR-ABL and NUP98-HOXA9 proliferate slower than similarly treated wild-type cells improving host survival


Mouse Genome Informatics
cn13
    Cd19tm1(cre)Cgn/Cd19+
Rbpjtm1Hon/Rbpjtm1Hon

involves: 129P2/OlaHsd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mutants show high sensitivity to the lethal effects of blood-borne Staphylococcus aureus infection

immune system
• loss of marginal zone B cells (J:76240)
• decreased numbers of marginal zone B cells (J:121523)
• increased numbers of follicular B cells in spleen (J:121523)
• affected differentiation of T2 B cells, indicated by a reduced population of marginal zone B (MZB) cells and an increased population of follicular B cells
• 3-fold increase in serum IgG3
• increased mortality rate after blood-born bacterial infection
• mutants show high sensitivity to the lethal effects of blood-borne Staphylococcus aureus infection

hematopoietic system
• loss of marginal zone B cells (J:76240)
• decreased numbers of marginal zone B cells (J:121523)
• increased numbers of follicular B cells in spleen (J:121523)
• affected differentiation of T2 B cells, indicated by a reduced population of marginal zone B (MZB) cells and an increased population of follicular B cells
• 3-fold increase in serum IgG3


Mouse Genome Informatics
cn14
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Nes-cre)1Wme/0

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
cellular
• cysts result from aberrant fate determination of stem cells to epidermal progenitors and their accelerated differentiation into epidermis

integument
• begin to lose hair 1-3 months after birth, when the first or second hair cycle terminates
• hair loss frequently occurs in the vibrissa region
• hyperkeratinization of the epidermis on the back and tail
• epidermis is thickened in some regions
• cysts in the dermal layer but not in the epidermis, close to hair follicles, frequently in the vibrissa region
• cysts are composed of epidermal cells aberrantly differentiated from mutant hair follicular stem cells
• cyst formation occurs after hair loss or after the first hair cycle


Mouse Genome Informatics
cn15
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Cyp1a1-cre)1Dwi/0

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
digestive/alimentary system
• 5 days after injection with beta-naphthoflavone, essentially all epithelial cell division has stopped in the small intestine and colon, but no significant increase in apoptosis is seen
• 5 days after injection with beta-naphthoflavone, in the small intestine and colon the rapidly dividing transit amplifying compartment is completely replaced by post-mitotic goblet cells
• however, in the small intestine Paneth cell morphology is normal
• 3 and 5 days after injection with beta-naphthoflavone the number of goblet cells is increased in the small intestine and colon

endocrine/exocrine glands
• 5 days after injection with beta-naphthoflavone, in the small intestine and colon the rapidly dividing transit amplifying compartment is completely replaced by post-mitotic goblet cells
• however, in the small intestine Paneth cell morphology is normal


Mouse Genome Informatics
cn16
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Mx1-cre)1Cgn/0

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of PDCs is elevated in the spleen
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, proportion of conventional Cd11chigh MHC class II+ dendritic cells is reduced 2.5 fold; decrease in the fraction of Cd11b+ dendritic cells corresponding to the CD8- subset is consistently observed
• one month after IFN-induced deletion, mutants show a gradual deletion of marginal zone B cells, from 10% to 3% of splenic B cells (J:76240)
• after poly(I)-poly(C) injection, marginal zone B cells are drastically reduced in spleen (J:99747)
• 1 and 3 months after poly(I)-poly(C) injection, a progressive increase in the number of B cells is seen in the thymus (J:99747)
• after poly(I)-poly(C) injection, pro-B and pre-B cell numbers are increased in the thymus (J:99747)
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, increased B cell development in the thymus is observed (J:125869)
• after poly(I)-poly(C) injection, follicular B cells are slightly increased in spleen
• 1 and 3 months after poly(I)-poly(C) injection, a progressive decrease in the number of single positive cells is seen in the thymus
• 1 month after poly(I)-poly(C) injection, the number of thymocytes is reduced and by 3 months the number of thymocytes is reduced to 1/8 that in control mice
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of Cd11b+ Cd11c- monocytes/macrophages is elevated in the spleen
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of Cd11b+ Cd11c- monocytes/macrophages is elevated in the spleen
• 1 and 3 months after poly(I)-poly(C) injection, in the thymus and bone marrow lymphocyte progenitors induce B cell development at the expense of T cell development; however myeloid and B cell lineage development in the bone marrow is normal
• 1 and 3 months after poly(I)-poly(C) injection, a progressive decrease in the number of double positive cells is seen in the thymus
• 1 and 3 months after poly(I)-poly(C) injection, a progressive increase in the number of double negative cells is seen in the thymus; however many of these cells are B cells
• after poly(I)-poly(C) injection, B cell development is impaired in the spleen but not in the bone marrow
• 1 and 3 months after poly(I)-poly(C) injection, T cell development is arrested at the CD44+CD25- pro-T cell or earlier stage (J:99747)
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, mice manifest a block in T cell development in the thymus (J:125869)
• 1 and 3 months after poly(I)-poly(C) injection, thymus size is reduced
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, there is decreased splenic dendritic cell content

hematopoietic system
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of PDCs is elevated in the spleen
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, proportion of conventional Cd11chigh MHC class II+ dendritic cells is reduced 2.5 fold; decrease in the fraction of Cd11b+ dendritic cells corresponding to the CD8- subset is consistently observed
• one month after IFN-induced deletion, mutants show a gradual deletion of marginal zone B cells, from 10% to 3% of splenic B cells (J:76240)
• after poly(I)-poly(C) injection, marginal zone B cells are drastically reduced in spleen (J:99747)
• 1 and 3 months after poly(I)-poly(C) injection, a progressive increase in the number of B cells is seen in the thymus (J:99747)
• after poly(I)-poly(C) injection, pro-B and pre-B cell numbers are increased in the thymus (J:99747)
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, increased B cell development in the thymus is observed (J:125869)
• after poly(I)-poly(C) injection, follicular B cells are slightly increased in spleen
• 1 and 3 months after poly(I)-poly(C) injection, a progressive decrease in the number of single positive cells is seen in the thymus
• 1 month after poly(I)-poly(C) injection, the number of thymocytes is reduced and by 3 months the number of thymocytes is reduced to 1/8 that in control mice
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of Cd11b+ Cd11c- monocytes/macrophages is elevated in the spleen
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, fraction of Cd11b+ Cd11c- monocytes/macrophages is elevated in the spleen
• 1 and 3 months after poly(I)-poly(C) injection, in the thymus and bone marrow lymphocyte progenitors induce B cell development at the expense of T cell development; however myeloid and B cell lineage development in the bone marrow is normal
• 1 and 3 months after poly(I)-poly(C) injection, a progressive decrease in the number of double positive cells is seen in the thymus
• 1 and 3 months after poly(I)-poly(C) injection, a progressive increase in the number of double negative cells is seen in the thymus; however many of these cells are B cells
• after poly(I)-poly(C) injection, B cell development is impaired in the spleen but not in the bone marrow
• 1 and 3 months after poly(I)-poly(C) injection, T cell development is arrested at the CD44+CD25- pro-T cell or earlier stage (J:99747)
• 3 weeks after induction of cre expression by poly(I):poly(C) treatment, mice manifest a block in T cell development in the thymus (J:125869)
• 1 and 3 months after poly(I)-poly(C) injection, thymus size is reduced


Mouse Genome Informatics
cn17
    Cd19tm1(cre)Cgn/Cd19+
Rbpjtm1Hon/Rbpjtm1Hon
Spentm2.1Hon/Spentm2.1Hon

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• severely decreased numbers of marginal zone B cells in spleen
• increased numbers of follicular B cells in spleen

hematopoietic system
• severely decreased numbers of marginal zone B cells in spleen
• increased numbers of follicular B cells in spleen


Mouse Genome Informatics
cn18
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Itgax-cre)1-1Reiz/0

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
hematopoietic system
• splenic dendritic cell (DC) population is decreased similarly to bone marrow deletion of Rbpj; ratio of CD8-/CD8+ dendritic cells is significantly decreased, resulting from 3-fold decrease in numbers of CD8- DCs with normal numbers of CD8+ DCs
• frequency of cytokine-secreting CD8- DCs in cultured splenocytes is decreased 3-fold compared to controls whereas cytokine-secreting CD8+ DCs is unchanged

immune system
• splenic dendritic cell (DC) population is decreased similarly to bone marrow deletion of Rbpj; ratio of CD8-/CD8+ dendritic cells is significantly decreased, resulting from 3-fold decrease in numbers of CD8- DCs with normal numbers of CD8+ DCs
• frequency of cytokine-secreting CD8- DCs in cultured splenocytes is decreased 3-fold compared to controls whereas cytokine-secreting CD8+ DCs is unchanged
• in response to TLR activation, dendritic cell cytokine production is decreased compared to controls


Mouse Genome Informatics
cn19
    Mesp1tm2(cre)Ysa/Mesp1+
Rbpjtm1Hon/Rbpjtm1Hon

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
cardiovascular system
• valve formation is abnormal due to a lack of epithelial-mesenchymal transition and defective trabeculae
• trabecular layers are underdeveloped
• endocardial layers are abnormally detached from myocardial layers


Mouse Genome Informatics
cn20
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Lck-cre)19Hhan/0

involves: 129P2/OlaHsd * C57BL/6 * DBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
N
• mice show normal T cell development in the thymus and periphery (J:168475)
• engrafted mice show a nearly 2-fold increase in absolute number of T cells, while the B cell compartment is not different from wild-type
• in draining lymph nodes, a significant increase in CD4+ and CD8+ T cells is detected
• animals exhibit a significant increase in macrophage antigen-1-positive cells in spleens
• mice show 1.5-fold increase splenocyte number in engrafted mice
• skin and cardiac allografts from BALB/c mice to Rbpj-deficient mice show decreased survival time
• grafts of both tissue type show increased leukocyte infiltration
• percentage of proliferating CD4+ cells is significantly increased in lymph nodes and spleen; a similar increase in CD8+ T cells in spleen and lymph nodes is observed in engrafted animals
• suppression activity of CD4+CD25+ regulatory T cells is significantly attenuated in vitro; generation of Treg cells in vivo in graft recipients is not altered

hematopoietic system
• engrafted mice show a nearly 2-fold increase in absolute number of T cells, while the B cell compartment is not different from wild-type
• in draining lymph nodes, a significant increase in CD4+ and CD8+ T cells is detected
• animals exhibit a significant increase in macrophage antigen-1-positive cells in spleens
• percentage of proliferating CD4+ cells is significantly increased in lymph nodes and spleen; a similar increase in CD8+ T cells in spleen and lymph nodes is observed in engrafted animals
• mice show 1.5-fold increase splenocyte number in engrafted mice
• suppression activity of CD4+CD25+ regulatory T cells is significantly attenuated in vitro; generation of Treg cells in vivo in graft recipients is not altered


Mouse Genome Informatics
cn21
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Vil-cre/ERT2)23Syr/0

involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
digestive/alimentary system
• 6 days after the last injection of tamoxifen (given for 5 consecutive days), in the small intestine and colon the rapidly dividing transit amplifying compartment is replaced by post-mitotic goblet cells, with almost complete conversion by 12 days

endocrine/exocrine glands
• 6 days after the last injection of tamoxifen (given for 5 consecutive days), in the small intestine and colon the rapidly dividing transit amplifying compartment is replaced by post-mitotic goblet cells, with almost complete conversion by 12 days


Mouse Genome Informatics
cn22
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Tyr-cre)2Lru/0

involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
pigmentation
N
• pigmentation mediated by non-follicular melanocytes such as in the ear or eye is not affected by this mutation (J:116658)
• almost complete hair whitening is observed

integument
• almost complete hair whitening is observed


Mouse Genome Informatics
cn23
    Rbpjtm1Hon/Rbpj+
Tg(Tyr-cre)2Lru/0

involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
pigmentation
N
• loss of one allele has no affect on coat color; no graying or dilution is observed in 8-week old mice (J:116658)


Mouse Genome Informatics
cn24
    Fbxw7tm1Kei/Fbxw7tm1Kei
Rbpjtm1Hon/Rbpjtm1Hon
Tg(Cd4-cre)1Cwi/?

involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• the number and percent of double positive thymocytes is increased compared to in wild-type mice

hematopoietic system
• the number and percent of double positive thymocytes is increased compared to in wild-type mice


Mouse Genome Informatics
cn25
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(MMTV-cre)1Mam/0

involves: 129P2/OlaHsd * C57BL/6J * FVB * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
endocrine/exocrine glands
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris (J:108712)
• on day 11, epithelia appears much sparser (J:108712)
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material (J:108712)
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei (J:108712)
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy (J:108712)
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution (J:108712)

reproductive system
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris (J:108712)
• on day 11, epithelia appears much sparser (J:108712)
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material (J:108712)
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei (J:108712)
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy (J:108712)
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution (J:108712)

integument
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris (J:108712)
• on day 11, epithelia appears much sparser (J:108712)
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material (J:108712)
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei (J:108712)
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy (J:108712)
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution (J:108712)
• mutant skin display hyperkeratinization
• cysts containing abundant whorls of keratin are observed


Mouse Genome Informatics
cn26
    Nr2f2tm2.1Tsa/Nr2f2tm2.1Tsa
Rbpjtm1Hon/Rbpjtm1Hon
Tg(Tek-cre)1Ywa/0

involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• fewer embryos than expected are found at E10.0

immune system
• at E10.0, number of Prox1+ve lymphatic endothelial cells is dramatically reduced


Mouse Genome Informatics
cn27
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Tek-cre)1Ywa/0

involves: 129S7/SvEvBrd * C57BL/6 * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• fewer embryos than expected are found at E10.0


Mouse Genome Informatics
cx28
    Rbpjtm1Hon/Rbpjtm1Hon
Tg(Cd4-cre)1Cwi/?

involves: 129P2/OlaHsd * C57BL/6 * DBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
hematopoietic system
• na´ve T cells fail to differentiate into the Th2 sub-type when co-cultured with LPS-treated DC from Myd88-null mice
• addition of IL-4 rescued this defect

immune system
• na´ve T cells fail to differentiate into the Th2 sub-type when co-cultured with LPS-treated DC from Myd88-null mice
• addition of IL-4 rescued this defect