Mouse Genome Informatics
hm1
    Ppargtm1Lja/Ppargtm1Lja
involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype

Embryonic lethality of Ppargtm1Lja/Ppargtm1Lja mice

mortality/aging

embryogenesis
• smaller at E10.5-11.5

growth/size
• smaller at E10.5-11.5


Mouse Genome Informatics
ht2
    Ppargtm1Lja/Pparg+
involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype

Hepatic steatosis in Ppargtm1Lja/Pparg+ mice

adipose tissue
• reduced fat mass
• exhibited lipodystrophy
• brown adipocytes contained large multiloculated fat vacuoles not present in wildtype
• epididymal white adipose tissue contained a heterogeneous population of adipocytes with mostly small and medium sized cells, compared to the homozygous population of large adipocytes in wildtype
• reduced epididymal white adipose tissue depots

growth/size
• reduced fat mass
• exhibited lipodystrophy
• heterozygous mutants gained less weight on a standard or high fat diet than wildtype after 3 months of age, however no differences in food intake or oxygen consumption

homeostasis/metabolism
• males on a high-fat diet exhibited basal hyperinsulinemia
• exhibited higher serum free fatty acid levels on a standard diet than wildtype
• males on a high-fat diet exhibited insulin resistance, as evidenced by lower glucose infusion rates and impaired glucose uptake in skeletal muscle during clamp studies
• significantly lower levels of serum adiponectin levels than in wildtype on either a standard or high fat diet
• hepatic triglyceride levels were 1.8-fold greater on a standard diet and 1.46-fold greater on a high-fat diet compared to wildtype

muscle
• males on a high-fat diet display impaired glucose uptake in skeletal muscle during clamp studies

liver/biliary system
• hepatic triglyceride levels were 1.8-fold greater on a standard diet and 1.46-fold greater on a high-fat diet compared to wildtype
• 5 month old males exhibited macrovesicular fatty changes in midzonal and centrilobular locations and microvesicular fatty changes around the central vein, indicating hepatic steatosis

cardiovascular system
• females, but not males, were overtly hypertensive on either the standard or high-fat diet

cellular
• males on a high-fat diet display impaired glucose uptake in skeletal muscle during clamp studies