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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tgif1tm1.1Caw
targeted mutation 1.1, Christopher A Walsh
MGI:3577049
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Tgif1tm1.1Caw/Tgif1tm1.1Caw B6.129S-Tgif1tm1.1Caw MGI:5516484
hm2
Tgif1tm1.1Caw/Tgif1tm1.1Caw involves: C57BL/6 * FVB/N MGI:3577403
ht3
Tgif1tm1.1Caw/Tgif1+ B6.129S-Tgif1tm1.1Caw MGI:5516482


Genotype
MGI:5516484
hm1
Allelic
Composition
Tgif1tm1.1Caw/Tgif1tm1.1Caw
Genetic
Background
B6.129S-Tgif1tm1.1Caw
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1.1Caw mutation (0 available); any Tgif1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer (10.84%) than the expected 25% of mutants are seen at weaning from heterozygous intercrosses
• fewer (27.9%) than the expected 50% of homozygotes are seen at weaning from heterozygous female to homozygous male crossings, indicating embryonic or neonatal lethality

embryo
• central regions of the placental labyrinths are less well developed
• reduction in maternal blood spaces in placentas
• the maternal blood spaces appear larger with fewer surrounding fetal vessels

growth/size/body

hearing/vestibular/ear
• mucus-type ear effusion at 2 months of age, with a high density of goblet cells in the epithelium of the middle ear cavity
• increase in mucosal thickness of the middle ear
• polyps that arise from the middle ear mucosa are seen at 4 months of age
• the average auditory-evoked brainstem response thresholds are elevated by about 30 dB SPL in mutants at 2 months of age, suggesting conductive hearing loss
• 50% of mutants have reduced hearing and 14% have almost normal hearing, although still reduced compared to wild-type mice, at 2 months of age
• however, no abnormalities are seen in the cochlea or organ of Corti and no loss of spinal ganglion neurons is seen
• the average auditory-evoked brainstem response thresholds are elevated by about 30 dB SPL in mutants at 2 months of age, suggesting conductive hearing loss
• 36% of mice are deaf at 2 months of age
• mutants exhibit otitis media by 21 days of age, with 67% of mutants having fluid in the middle ear cavity and a thickened epithelial lining
• inflammation progresses to a chronic inflammation with effusion by 2 months of age
• appearance of cellular debris-like aggregates in the ear effusions at 2 and 4 months of age
• middle ear fluid contains granulocytes, monocytes, and macrophages and the infammatory cell populations vary over time

behavior/neurological
• 86% of mutants show a reduced Preyer's reflex at 1 month of age, and all mutants show reduced hearing by 2 months of age

craniofacial

homeostasis/metabolism
• mutants exhibit ear effusions at P21 that develop into mucus-type effusions at 2 months of age
• levels of cytokines, TNF-alpha and IL-1beta, are elevated in ear fluids

immune system
• mutants exhibit otitis media by 21 days of age, with 67% of mutants having fluid in the middle ear cavity and a thickened epithelial lining
• inflammation progresses to a chronic inflammation with effusion by 2 months of age
• appearance of cellular debris-like aggregates in the ear effusions at 2 and 4 months of age
• middle ear fluid contains granulocytes, monocytes, and macrophages and the infammatory cell populations vary over time
• levels of cytokines, TNF-alpha and IL-1beta, are elevated in ear fluids

nervous system
• 11 of 67 mutants develop hydrocephalus at 11 months of age

skeleton

reproductive system
• by E13.5, the number of resorbed embryos is increased and only 56.25% of embryos appear normal
• only 1 of 5 females gives birth to pups when mated with homozygous males

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
otitis media DOID:10754 OMIM:166760
J:198238




Genotype
MGI:3577403
hm2
Allelic
Composition
Tgif1tm1.1Caw/Tgif1tm1.1Caw
Genetic
Background
involves: C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1.1Caw mutation (0 available); any Tgif1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• displayed normal growth and behavior and were fertile and there were no discernible derangements in any of the major organ systems, including the forebrain




Genotype
MGI:5516482
ht3
Allelic
Composition
Tgif1tm1.1Caw/Tgif1+
Genetic
Background
B6.129S-Tgif1tm1.1Caw
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1.1Caw mutation (0 available); any Tgif1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• central regions of the placental labyrinths are less well developed
• reduction in maternal blood spaces in placentas
• the maternal blood spaces appear larger with fewer surrounding fetal vessels

hearing/vestibular/ear
• 19% of mutants have fluid in one ear and thickened epithelial lining in the middle ear at P21
• 37% of mutants at P21 exhibit thickened epithelial lining in the middle ear only
• the average auditory-evoked brainstem response thresholds are elevated by about 20 dB SPL in mutants at 2 months of age
• 27% of mutants have slightly reduced hearing at 2 months of age

nervous system
• 4 of 199 heterozygotes develop hydrocephalus at 11 months of age





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last database update
03/14/2017
MGI 6.08
The Jackson Laboratory