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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(KRT5-tTA)1216Glk
transgene insertion 1216, Adam Glick
MGI:3575755
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-Il13)1Tazh/0
B6.Cg-Tg(KRT5-tTA)1216Glk Tg(tetO-Il13)1Tazh MGI:3850190
cx2
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-Tek)1Dmt/0
involves: CD-1 * FVB/N MGI:3842959
cx3
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-TGFB1*C223S*C225S)1Glk/0
involves: FVB/N MGI:3815534
cx4
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-LMNA*G608G,-EGFP)VF1-07Maer/0
involves: FVB/N MGI:3834667
cx5
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-LMNA,-EGFP)SF1-04Maer/0
involves: FVB/N MGI:3834668


Genotype
MGI:3850190
cx1
Allelic
Composition
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-Il13)1Tazh/0
Genetic
Background
B6.Cg-Tg(KRT5-tTA)1216Glk Tg(tetO-Il13)1Tazh
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• 4-fold in the skin following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal and stimulation with anti-CD3/CD28, lymphocytes and peripheral blood mononuclear cells produce more IL13 than wild-type cells
• following doxycycline withdrawal, IL4 lymphocyte production is increased compared to in wild-type mice
• following doxycycline withdrawal, mice exhibit increased numbers of mononuclear cells and eosinophils infiltrating the subepidermal, intradermal, and perivascular spaces of the skin unlike in wild-type mice

cardiovascular system
• following doxycycline withdrawal, mice exhibit an increase in subcutaneous blood vessel formation compared to in wild-type mice

hematopoietic system
• 4-fold in the skin following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal

homeostasis/metabolism
• spongiosis following doxycycline withdrawal

integument
• following doxycycline withdrawal, mice exhibit increased numbers of mononuclear cells and eosinophils infiltrating the subepidermal, intradermal, and perivascular spaces of the skin unlike in wild-type mice
• following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal
• following doxycycline withdrawal, skin lesions progress and become extensive including dry lichenified skin lesions unlike in wild-type mice
• following doxycycline withdrawal, mice exhibit skin crusting, excoriation, bacterial pyoderma, and erosions in the skin mostly on the back and abdominal areas unlike wild-type mice
• spongiosis following doxycycline withdrawal
• following doxycycline withdrawal, mice develop skin fibrosis unlike wild-type mice
• following doxycycline withdrawal, mice exhibit pruritus unlike wild-type mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
atopic dermatitis DOID:3310 OMIM:603165
OMIM:PS603165
J:150232




Genotype
MGI:3842959
cx2
Allelic
Composition
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-Tek)1Dmt/0
Genetic
Background
involves: CD-1 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• is increased in the ear and back skin of mice
• interleukin-22 secretion is increased in the ear and back skin of mice
• is increased in the ear and back skin of mice
• is increased in the ear and back skin of mice
• is increased in the ear and back skin of mice
• inflammatory infiltrate associated with psoriasis includes CD4+ T cells in the dermis and CD8+ T cells in the epidermis, myeloid dendritic cells, macrophages and granulocytes

cardiovascular system
• mouse ears by 21 days of age contain larger blood vessels that are more highly branched
• a similar trend is observed for vessels in both dorsal and ventral superficial fascia
• ears contain 59% more blood vessels and back skin 102% more blood vessels than controls

integument
• inflammatory infiltrate associated with psoriasis includes CD4+ T cells in the dermis and CD8+ T cells in the epidermis, myeloid dendritic cells, macrophages and granulocytes
• is observed in the skin and back
• mouse ears are erythematous by 21 days of age
• skin begins to develop reddened, scaly, hyperkeratotic lesions between 2 to 6 months of age
• lesions involve hyperplasia of the epidermis, a loss of the granular cell layer, thickening of the interfollicular epidermal layers, confluent parakeratotic scale, increased dermal angiogenesis, and extensive inflammatory infiltration
• dorsal and ventral surfaces, bilateral elbows, and skin surrounding the genital region can develop these lesion
• 73% of mice have lesions involving back and ear, 22% develop just ear lesions, and 5% develop no lesions
• psoriasis can be reversed by treatment with doxycycline or attenuated with cyclosporine A treatment
• the epidermis is 3.2- and 3.1- fold thicker in the ear and backs, respectively, than in controls

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
psoriasis DOID:8893 OMIM:PS177900
J:147438




Genotype
MGI:3815534
cx3
Allelic
Composition
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-TGFB1*C223S*C225S)1Glk/0
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• when pregnant mice are maintained on 10 ug/ml or higher doxycycline, double-transgenic mice with normal morphology are born at expected ratios
• 1-5 ug/ml doxycycline treatment during gestation allows full-term development of double-transgenic mice, but pups are born dead or die shortly after birth
• no live animals are obtained in litters born when the pregnant mouse does not receive doxycycline during gestation

growth/size/body
• animals born dead or dying shortly after birth with 1-5 ug/ml doxycycline treatment during gestation are runted

immune system
• weanlings treated by doxycycline withdrawal displays a variable neutrophilic infiltrate characterized by microabcesses within and above cornified layers
• weanlings treated by doxycycline withdrawal develop dermatitis on scalp and shoulders, as well as ventral surface; dermatitis extends caudally with time

cellular
• cell proliferation in the epidermis is significantly reduced in double transgenic newborns after 1-5 ug/ml doxycycline treatment during gestation

integument
• abnormalities manifest several weeks later than in Tg(tetO-TGFB1*C223S*C225S)1Glk/Tg(KRT5-rtTA)1Glk double-transgenic mice treated with doxycycline
• with reintroduction of doxycycline at >5 ug/ml in diet, nearly complete hair regrowth is observed within 14 days
• weanlings treated by doxycycline withdrawal display progressive hair loss
• in weanlings treated by doxycycline withdrawal, many hair follicles appear hyperplastic with abnormal morphologies
• hair follicle density is reduced in affected newborns after 1-5 ug/ml doxycycline treatment during gestation
• hyperkeratosis and other abnormalities manifest several weeks later than in Tg(tetO-TGFB1*C223S*C225S)1Glk/Tg(KRT5-rtTA)1Glk double-transgenic mice treated with doxycycline
• with reintroduction of doxycycline at >5 ug/ml in diet, hyperkeratotic phenotype is reversed
• dermal layer in affected double-transgenic newborns is hypervascular after 1-5 ug/ml doxycycline treatment during gestation
• significant thickening is observed in weanlings treated by doxycycline withdrawal
• affected double-transgenic newborns after 1-5 ug/ml doxycycline treatment during gestation have reduced number of cornified layers
• weanlings treated by doxycycline withdrawal develop hyperkeratosis
• epidermis of affected mice (weanlings treated by doxycycline withdrawal) becomes acanthotic
• epidermis of affected mice (weanlings treated by doxycycline withdrawal) exhibits hyperplasia
• epidermis is thinner in affected double-transgenic newborns than single-transgenic pups
• animals born dead or dying shortly after birth with 1-5 ug/ml doxycycline treatment during gestation have shiny tight erythemic skin
• abnormalities manifest several weeks later than in Tg(tetO-TGFB1*C223S*C225S)1Glk/Tg(KRT5-rtTA)1Glk double-transgenic mice treated with doxycycline
• weanlings treated by doxycycline withdrawal displays a variable neutrophilic infiltrate characterized by microabcesses within and above cornified layers
• weanlings treated by doxycycline withdrawal develop dermatitis on scalp and shoulders, as well as ventral surface; dermatitis extends caudally with time




Genotype
MGI:3834667
cx4
Allelic
Composition
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-LMNA*G608G,-EGFP)VF1-07Maer/0
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• median survival is 14 weeks for mice withdrawn from doxycycline at 21 days and 7 weeks for those withdrawn at P0
• median survival can be increased from 7 weeks to 29 by providing mice with soft pellets on the cage floor

skeleton
• following doxycycline withdrawal at birth, mice exhibit changes in the lower incisors and surrounding tissue unlike in wild-type mice

craniofacial
• following doxycycline withdrawal at birth, mice exhibit increased impaction of food or bedding material with acute inflammation to overt necrosis of the pulp, accumulation of cellulose in the pulp, and spread of inflammation into the periodontal ligament and surrounding bony structure unlike in wild-type mice
• following doxycycline withdrawal at birth, mice exhibit changes in the lower incisors and surrounding tissue unlike in wild-type mice

digestive/alimentary system
• following doxycycline withdrawal, mice exhibit hypoplasia in the stomach unlike in wild-type mice

growth/size/body
• following doxycycline withdrawal at birth, mice exhibit increased impaction of food or bedding material with acute inflammation to overt necrosis of the pulp, accumulation of cellulose in the pulp, and spread of inflammation into the periodontal ligament and surrounding bony structure unlike in wild-type mice
• following doxycycline withdrawal at birth, mice exhibit changes in the lower incisors and surrounding tissue unlike in wild-type mice
• at 7 weeks of age in mice withdrawn from doxycycline at birth

endocrine/exocrine glands
• six weeks after withdrawal of doxycycline, sebaceous glands exhibit irregular maturation of sebocytes unlike in wild-type mice
• six weeks after withdrawal of doxycycline, sebaceous glands appear enlarged and displaced
• six weeks after withdrawal of doxycycline, sebaceous glands exhibit hyperplasia unlike in wild-type mice
• 17 weeks after doxycycline withdrawal, sebaceous glands appear hypoplastic

immune system
• following doxycycline withdrawal at birth, mice exhibit spread of inflammation into the periodontal ligament and surrounding bony structure unlike in wild-type mice
• however, no changes were seen in the upper jaws
• six weeks after withdrawal of doxycycline, inflammatory cells infiltrate the dermis unlike in wild-type mice

integument
• six weeks after withdrawal of doxycycline, sebaceous glands exhibit irregular maturation of sebocytes unlike in wild-type mice
• six weeks after withdrawal of doxycycline, sebaceous glands appear enlarged and displaced
• six weeks after withdrawal of doxycycline, sebaceous glands exhibit hyperplasia unlike in wild-type mice
• 17 weeks after doxycycline withdrawal, sebaceous glands appear hypoplastic
• six weeks after withdrawal of doxycycline, inflammatory cells infiltrate the dermis unlike in wild-type mice
• following doxycycline withdrawal, mice have thin coats
• however, hair follicle number is normal
• absent 17 weeks after withdrawal of doxycycline
• six weeks after withdrawal of doxycycline
• six weeks after withdrawal of doxycycline, inflammatory cells infiltrate the dermis unlike in wild-type mice
• 17 weeks after doxycycline withdrawal, the dermis exhibits fibrosis unlike in wild-type mice
• six weeks after withdrawal of doxycycline
• six weeks after withdrawal of doxycycline, mice exhibit hypergranulosis unlike wild-type mice
• six weeks after withdrawal of doxycycline, mice exhibit slight to moderate hyperplasia of the interfollicular epidermis unlike in wild-type mice
• after withdrawal of doxycycline, some areas exhibit severe hyperplasia unlike in wild-type mice
• six weeks after withdrawal of doxycycline, mice exhibit skin lesions of varying severity unlike in wild-type mice
• 17 weeks after doxycycline withdrawal, the dermis exhibits fibrosis unlike in wild-type mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
progeria DOID:3911 OMIM:176670
J:145312




Genotype
MGI:3834668
cx5
Allelic
Composition
Tg(KRT5-tTA)1216Glk/0
Tg(tetO-LMNA,-EGFP)SF1-04Maer/0
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
phenotype observed in females
phenotype observed in males
N normal phenotype
integument
N
• skin morphology is normal following doxycycline withdrawal





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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory