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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Agrptm2(DTR)Rpa
targeted mutation 2, Richard D Palmiter
MGI:3527177
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Agrptm2(DTR)Rpa/Agrptm2(DTR)Rpa involves: 129S4/SvJaeSor MGI:3776094
hm2
Agrptm2(DTR)Rpa/Agrptm2(DTR)Rpa involves: 129S4/SvJaeSor * C57BL/6 MGI:3776082
ht3
Agrptm2(DTR)Rpa/Agrp+ involves: 129S4/SvJaeSor * C57BL/6 MGI:3776081
cn4
Agrptm2(DTR)Rpa/Agrp+
Grin1tm2Stl/Grin1tm2Stl
involves: 129S4/SvJae * 129S4/SvJaeSor MGI:5427451
cn5
Agrptm2(DTR)Rpa/Agrp+
Tph2tm1Mscl/Tph2tm1Mscl
involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * C57BL/6NCr MGI:5427450
cn6
Agrptm2(DTR)Rpa/Agrp+
Slc17a6tm1.1Thna/Slc17a6tm1.1Thna
involves: 129S4/SvJaeSor * C57BL/6J MGI:5427452
cx7
Agrptm2(DTR)Rpa/Agrp+
Lepob/Lepob
involves: 129S4/SvJaeSor * C57BL/6 MGI:5319636
cx8
Agrptm2(DTR)Rpa/Agrp+
Tg(Pomc-EGFP)1Low/0
involves: 129S4/SvJaeSor * C57BL/6J MGI:3776092
cx9
Ay/a
Agrptm2(DTR)Rpa/Agrp+
involves: 129S4/SvJaeSor * KK/Upj MGI:3776093


Genotype
MGI:3776094
hm1
Allelic
Composition
Agrptm2(DTR)Rpa/Agrptm2(DTR)Rpa
Genetic
Background
involves: 129S4/SvJaeSor
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• following treatment with diptheria toxin (DT) to specifically ablate NPY-expressing cells, mice fail to increase their food intake at a 10% loss of body weight and their intake dramatically decreased as their weight loss approaches 20%




Genotype
MGI:3776082
hm2
Allelic
Composition
Agrptm2(DTR)Rpa/Agrptm2(DTR)Rpa
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit a greater than 80% drop in feeding, a 20% reduction in their body weight and have to be euthanized unlike similarly treated wild-type mice
• however, most mice survive when injected with DT as neonate or when receiving injections as neonate and adults

behavior/neurological
• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit increased water consumption compared to similarly treated wild-type mice
• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit a greater than 80% drop in feeding unlike similarly treated wild-type mice
• adults injected with DT cease to eat unlike similarly treated wild-type mice
• however, DT-treated mice are sustained by hand feeding via oral gavage with liquid food

growth/size/body
• adult mice injected intramuscularly with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit 20% of their body weight unlike similarly treated wild-type mice




Genotype
MGI:3776081
ht3
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• adults injected with diptheria toxin (DT) to specifically ablate NPY-expressing cells cease to eat unlike similarly treated wild-type mice
• however, food consumption of neonates following injection with diptheria toxin to specifically ablate NPY-expressing cells is normal

growth/size/body
• neonates injection with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit an 11% decreased in body weight at weaning compared to similarly treated wild-type mice




Genotype
MGI:5427451
cn4
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Grin1tm2Stl/Grin1tm2Stl
Genetic
Background
involves: 129S4/SvJae * 129S4/SvJaeSor
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Grin1tm2Stl mutation (1 available); any Grin1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• diphtheria-treated mice bilaterally injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus

growth/size/body
• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice




Genotype
MGI:5427450
cn5
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Tph2tm1Mscl/Tph2tm1Mscl
Genetic
Background
involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * C57BL/6NCr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Tph2tm1Mscl mutation (0 available); any Tph2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries

behavior/neurological
• in diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries

growth/size/body
• diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries prevents starvation induced by AgRP neuron ablation unlike in control mice

homeostasis/metabolism
• in diphtheria-treated mice injected with a virus expressing a CAV2-cre into the nucleus tractus solitaries




Genotype
MGI:5427452
cn6
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Slc17a6tm1.1Thna/Slc17a6tm1.1Thna
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Slc17a6tm1.1Thna mutation (0 available); any Slc17a6 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• diphtheria-treated mice injected with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries or parabrachial nucleus prevents starvation induced by AgRP neuron ablation unlike in control mice
• mice injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus without ablation of AgRP neurons

behavior/neurological
• mice injected with a virus expressing a AAV1-CreGFP into the parabrachial nucleus whether treated with diphtheria or not
• in diphtheria treated mice bilaterally with a virus expressing a AAV1-CreGFP into the nucleus tractus solitaries




Genotype
MGI:5319636
cx7
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Lepob/Lepob
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Lepob mutation (5 available); any Lep mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• young adult mice stop eating and would succumb following treatment with diptheria toxin
• older moderately obese (35-40 g) mice survive diptheria toxin treatment despite weight loss
• however, mice that weigh over 40 g succumb following treatment with diptheria toxin
• norepinephrine treatment prevents prolongs the survival of diptheria toxin treated mice that weigh over 40 g

nervous system
• loss of agouti-related protein expressing neurons following treatment with diptheria toxin

behavior/neurological
• following treatment with diptheria toxin young adult mice gradually stop eating by day 6 post treatment
• older, moderately obese mice that are able to survive the weight loss resume feeding about 18 days after treatment

growth/size/body
• diptheria toxin treated older moderately obese mice weigh less than saline treated controls for several months after treatment
• following treatment with diptheria toxin mice lose 20% of their body weight

homeostasis/metabolism
• diptheria toxin treatment of mice that weigh over 40 g results in a sharp drop in body temperature to near ambient temperature as mice become moribund
• norepinephrine treatment prevents the drop in body temperature
• glucose tolerance is improved in diptheria toxin treated mice relative to saline treated controls and is not significantly different from mice heterozygous for Lepob alone

reproductive system
• following diptheria toxin treatment of older moderately obese mice, both males and females become fertile, unlike saline treated mice which remain infertile




Genotype
MGI:3776092
cx8
Allelic
Composition
Agrptm2(DTR)Rpa/Agrp+
Tg(Pomc-EGFP)1Low/0
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Tg(Pomc-EGFP)1Low mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• adult mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit 20% of their body weight unlike similarly treated wild-type mice

nervous system
• mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells exhibit miniature inhibitory postsynaptic currents that are 6-fold lower than in wild-type mice and lower than in fasted wild-type mice




Genotype
MGI:3776093
cx9
Allelic
Composition
Ay/a
Agrptm2(DTR)Rpa/Agrp+
Genetic
Background
involves: 129S4/SvJaeSor * KK/Upj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
a mutation (167 available); any a mutation (417 available)
Agrptm2(DTR)Rpa mutation (1 available); any Agrp mutation (15 available)
Ay mutation (11 available); any a mutation (417 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• in mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells

growth/size/body
• in mice treated with diptheria toxin (DT) to specifically ablate NPY-expressing cells





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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory