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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Prkcitm1Kido
targeted mutation 1, Yoshiaki Kido
MGI:3526850
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Prkcitm1Kido/Prkcitm1Kido
Tg(Ins2-cre)25Mgn/0
involves: 129/Sv * C57BL/6 * DBA/2 MGI:3527979
cn2
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Nphs1-cre)33Mska/0
involves: C57BL/6 * C57BL/6N * DBA/2 MGI:5295616
cn3
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Cryaa-cre)10Mlr/?
involves: FVB/N MGI:4417871
cn4
Prkcitm1Kido/Prkcitm1Kido
Tg(Cryaa-cre)10Mlr/?
involves: FVB/N MGI:4417872
cn5
Prkcitm1Kido/Prkcitm1Kido
Tg(Crx-cre)1Tfur/0
Not Specified MGI:3609222


Genotype
MGI:3527979
cn1
Allelic
Composition
Prkcitm1Kido/Prkcitm1Kido
Tg(Ins2-cre)25Mgn/0
Genetic
Background
involves: 129/Sv * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1Kido mutation (0 available); any Prkci mutation (69 available)
Tg(Ins2-cre)25Mgn mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• islet insulin secretion is increased at low glucose concentrations (3-fold at 2.8 mM glucose) but decreased at high glucose concentrations (about 50% less at 16.8 mM) compared to control
• alpha and beta cell area are normal as is the total insulin content of the islets

homeostasis/metabolism
• at 6 months, but not at 2 months, blood glucose concentrations are decreased in fasting mutants expressing cre compared to those without the cre transgene
• on a normal or high fat diet blood glucose concentration 60 minutes after the glucose load is significantly higher and insulin response to glucose is impaired; however, insulin tolerance is not impaired




Genotype
MGI:5295616
cn2
Allelic
Composition
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Nphs1-cre)33Mska/0
Genetic
Background
involves: C57BL/6 * C57BL/6N * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1.1Kido mutation (0 available); any Prkci mutation (69 available)
Prkcitm1Kido mutation (0 available); any Prkci mutation (69 available)
Tg(Nphs1-cre)33Mska mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Focal segmental glomerulosclerosis in Prkcitm1Kido/Prkcitm1.1Kido Tg(Nphs1-cre)33Mska/0 mice

mortality/aging
• mice exhibit a reduced lifespan with a median age of death of 6 weeks

growth/size/body
• mice exhibit growth retardation by 4 weeks of age

renal/urinary system
• partial detachment of podocytes with an irregular pattern of nephrin staining is seen at P10
• mice exhibit massive proteinuria at P0 and throughout their lives
• however, kidneys appear grossly normal at P0
• at P10, irregular adhesions between foot processes are observed
• at P10, effacement of foot processes is observed
• however, fine foot processes are noted at P0
• at P7-P10, the apico-basal cell polarity of podocytes is disturbed
• at P7, podocalyxin loses its apically restricted localization, whereas ZO-1 localizes at the irregular cell-cell junctions of podocytes
• at P10, irregular adhesions between foot processes do not form tight junctions
• at P7-P10, apically dislocated slit diaphragms are observed
• however, normal slit diaphragms are noted at P0
• loss of podocytes is noted by P21
• at P10, glomeruli display partial detachment of podocytes, occasional adhesion of glomeruli to Bowman's capsules, mesangial expansion, dilated capillaries, and an irregular pattern of nephrin staining
• at P10, glomerular capillaries are dilated
• by P21, glomeruli exhibit advanced segmental to global sclerotic lesions, consistent with severe renal dysfunction
• at P10, occasional adhesion of glomeruli to Bowman's capsules is observed
• at P0, PAS staining indicates occasional hyaline droplets, representing reabsorbed urinary protein, in proximal renal tubules
• at P0, PAS staining indicates occasional hyaline droplets, representing reabsorbed urinary protein, in proximal renal tubules

homeostasis/metabolism
• mice display a significantly increased serum creatinine level by 3 weeks of age
• mice display a significantly increased blood urea nitrogen level by 3 weeks of age
• mice exhibit massive proteinuria at P0 and throughout their lives
• however, kidneys appear grossly normal at P0

cardiovascular system
• at P10, glomerular capillaries are dilated

cellular
• partial detachment of podocytes with an irregular pattern of nephrin staining is seen at P10




Genotype
MGI:4417871
cn3
Allelic
Composition
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1.1Kido mutation (0 available); any Prkci mutation (69 available)
Prkcitm1Kido mutation (0 available); any Prkci mutation (69 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• pigment cells cover the surface of the lens
• small opaque pupils speckled with black material
• lenses are still normal at E10.5
• lens vesicle closes normally at E11.5
• lens epithelium layer thinner at E18.5 than for controls
• reduced cell proliferation in germinative zone of the lens epithelium
• increased expression of alpha smooth muscle actin in lens epithelia after E16.5
• fiber cell alignment severely disrupted at E18.5
• youngest lens fiber cells have lost apical connections
• interface between lens epithelial cells and fiber cells is much shorter at E12.5
• small almond shaped eyes
• smaller eyeball

pigmentation
• pigment cells cover the surface of the lens




Genotype
MGI:4417872
cn4
Allelic
Composition
Prkcitm1Kido/Prkcitm1Kido
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1Kido mutation (0 available); any Prkci mutation (69 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• pigment cells cover the surface of the lens
• small opaque pupils speckled with black material
• lenses are still normal at E10.5
• lens vesicle closes normally at E11.5
• lens epithelium layer thinner at E18.5 than for controls
• reduced cell proliferation in germinative zone of the lens epithelium
• increased expression of alpha smooth muscle actin in lens epithelia after E16.5
• fiber cell alignment severely disrupted at E18.5
• youngest lens fiber cells have lost apical connections
• interface between lens epithelial cells and fiber cells is much shorter at E12.5
• small almond shaped eyes
• smaller eyeball

pigmentation
• pigment cells cover the surface of the lens




Genotype
MGI:3609222
cn5
Allelic
Composition
Prkcitm1Kido/Prkcitm1Kido
Tg(Crx-cre)1Tfur/0
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1Kido mutation (0 available); any Prkci mutation (69 available)
Tg(Crx-cre)1Tfur mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• mild microphthalmia
• severe laminar disorganization of the entire retina with all types of cells, including mitotic progenitors and S-phase progenitors, randomly distributed
• apical junctions are not observed at the retinal apical edge nor around scattered photoreceptors indicating that adherens junction formation between the immature photoreceptors and the progenitors is lost
• photoreceptors are randomly distributed in the retina, indicating loss of photoreceptor polarity
• outer segment, inner segment, or synaptic terminal structures are absent although components of the outer segment, such as rhodopsin and S-opsin, and synaptic ribbons are detected
• absent although components such as are rhodopsin and S-opsin are detected
• absent outer segment

nervous system
• photoreceptors are randomly distributed in the retina, indicating loss of photoreceptor polarity
• outer segment, inner segment, or synaptic terminal structures are absent although components of the outer segment, such as rhodopsin and S-opsin, and synaptic ribbons are detected
• absent although components such as are rhodopsin and S-opsin are detected
• absent outer segment





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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory