Phenotypes associated with this allele
skeleton
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• calvaria cells from neonates and bone marrow stromal cells from adults produce fewer bone nodules than wild-type cells
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• at E15.5, ossification if diminished compared to in wild-type mice
• bone collars of long bones are shorter than in wild-type mice
• at E18.5, skulls exhibit decreased ossification compared to in wild-type mice
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cellular
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• calvaria cells from neonates and bone marrow stromal cells from adults produce fewer bone nodules than wild-type cells
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mortality/aging
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• all embryos die around E12.5 due to severe hemorrhaging within the central nervous system
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cardiovascular system
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• while vascularization has occurred within the ventral neural tube of control E10.5 embryos at the forelimb level, no such vascularization is observed in this region of mutant embryos
• in E12.5 embryos, endothelial cells and pericytes are absent from all ventral neural regions of the presumptive spinal cord except for in the floor plate
• in the dorsal neural tube of E12.5 embryos, endothelial cells and pericytes
form abnormal clusters and enlarged lumens in the vascular structures present
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• hemorrhaging is detected throughout the developing brain of E12.5 embryos
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• hemorrhaging is detected throughout the developing spine of E12.5 embryos
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• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos
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nervous system
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• hemorrhaging is detected throughout the developing brain of E12.5 embryos
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• hemorrhaging is detected throughout the developing spine of E12.5 embryos
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• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos
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mortality/aging
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• all embryos die around E12.5 due to severe hemorrhaging within the central nervous system
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cardiovascular system
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• the number of endothelial cells and pericytes present in the intraneural vascular plexus of E12.5 embryos is greatly reduced
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• hemorrhaging is detected throughout the developing brain of E12.5 embryos
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• hemorrhaging is detected throughout the developing spine of E12.5 embryos
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• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos
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nervous system
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• hemorrhaging is detected throughout the developing brain of E12.5 embryos
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• hemorrhaging is detected throughout the developing spine of E12.5 embryos
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• hemorrhaging and downregulation of the endothelial marker GLUT-1 suggest a defect in the blood brain barrier of developing embryos
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renal/urinary system
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• at E15.5 the collecting ducts are dilated and branch points are less evident
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Hoxb7-cre)13Amc mutation
(2 available)
Wnt7btm2.1Amc mutation
(0 available);
any
Wnt7b mutation
(17 available)
Wnt7btm2Amc mutation
(1 available);
any
Wnt7b mutation
(17 available)
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mortality/aging
renal/urinary system
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• at P10, urine osmolality is 56% that of controls
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• at P11 - P12 kidneys are grossly abnormal
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• by P11 - P12 kidneys are physiologically incompetent
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homeostasis/metabolism
mortality/aging
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• mice die within hours of birth
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respiratory system
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• lung epithelial and mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice
• however, normal cell differentiation and patterning are preserved
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• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice
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• the lung mesenchyme is thinner than in wild-type mice
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• the medial lobe bronchus of the right lung emanates from the cephalic lobe bronchus, unlike in wild-type mice
• however, the correct number of lobes is formed
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• lung weight is 50% of wild-type at E18.5 and 25% of wild-type at E14.5
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• lungs are poorly inflated
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• cartilaginous rings are incomplete
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homeostasis/metabolism
renal/urinary system
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• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells
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• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis
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• cell proliferation in the loop of Henle is significantly reduced
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• renal corpuscles abut the renal pelvis
• despite the absence of the medulla, kidneys are similar in size to controls
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• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis
• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells
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• at E15.5 and E16.5, the nascent medulla is absent indicating a failure to initiate medulla formation
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• at E18.5 the loop of Henle is truncated resembling morphology at an earlier stage prior to loop elongation
• cell proliferation in the loop of Henle is significantly reduced
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• at E18.5, less than a third of mice show hydroureter like swelling of the pelvic region
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skeleton
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• cartilaginous rings are incomplete
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cardiovascular system
cellular
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• at E17.5, a marked increase in the rate of apoptosis is seen in distal collecting duct epithelial cells
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• at E15.5, the majority of cells in the collecting duct divide along the radial axis unlike in controls where the majority of cells divide along the longitudinal axis
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• cell proliferation in the loop of Henle is significantly reduced
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• mesenchyme tip cell progenitors exhibit decreased replication compared to in wild-type mice
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Analysis Tools