Analysis Tools
immune system
|
• mutant T cells produce very little IFN-gamma when transferred into host that is immunized with hemagglutinin
|
|
Allele Symbol Allele Name Allele ID |
Tg(LCK-NFKBIA)5Dwb transgene insertion 5, Dean W Ballard MGI:3056807 |
||||||||||||||||
| Summary |
3 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mutant T cells produce very little IFN-gamma when transferred into host that is immunized with hemagglutinin
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A
• this is observed for both CD4+ and CD8+ T cells
• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8+ T cells
|
|
• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin
• thymocyte proliferation is also severely curtailed in response to other mitogens
• addition of IL-2 to culture does not rescue the proliferation defect
• similar results were observed for B cell-depleted splenocytes
|
|
• numbers of CD8 single-positive thymocytes bearing high levels of TCR are reduced by 40% compared to controls
|
|
• CD4+ T cell numbers are reduced in the spleen, lymph nodes, and blood though not to the degree of reduction observed for CD8+ T cells
|
|
• CD8+ T cell numbers are reduced by two-thirds or more in the spleen, lymph nodes, and blood
|
|
• thymocytes produce about 20-fold less IL-2 when stimulated compared to wild-type controls
• likewise, splenocytes only produce 8% the IL-2 as controls when stimulated
|
|
• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A
• this is observed for both CD4+ and CD8+ T cells
• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8+ T cells
|
|
• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin
• thymocyte proliferation is also severely curtailed in response to other mitogens
• addition of IL-2 to culture does not rescue the proliferation defect
• similar results were observed for B cell-depleted splenocytes
|
|
• numbers of CD8 single-positive thymocytes bearing high levels of TCR are reduced by 40% compared to controls
|
|
• CD4+ T cell numbers are reduced in the spleen, lymph nodes, and blood though not to the degree of reduction observed for CD8+ T cells
|
|
• CD8+ T cell numbers are reduced by two-thirds or more in the spleen, lymph nodes, and blood
|
|
• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A
• this is observed for both CD4+ and CD8+ T cells
• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8+ T cells
|
|
• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin
• thymocyte proliferation is also severely curtailed in response to other mitogens
• addition of IL-2 to culture does not rescue the proliferation defect
• similar results were observed for B cell-depleted splenocytes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• the decreased delayed type hypersensitivity response to antigen rechallenge and the decreased IFN-gamma secretion by T cells isolated from the draining lymph nodes to these rechallenged areas demonstrate a defective Th1 response
• defective class switching of antigen-specific immunoglobulin to IgG2a gives further evidence of a defective Th1-response
|
|
• antigen rechallenge in the lung leads to slightly less periarterial inflammation than in controls
• there is significant attenuation of airway hyperresponsiveness in these mice upon antigen rechallenge in the lung
• the number of cells isolated from BAL fluid in these aerosol rechallenged mice is one-third of controls
• the level of antigen-specific IgG2a in aerosol sensitized mice is markedly reduced
|
|
• antigenic challenge after prior sensitization leads to 4-fold less swelling in the footpads compared to controls
|
|
• T cells isolated from the draining lymph nodes of mice rechallenged with antigen produce significantly less IFN-gamma than controls
|
|
• the decreased delayed type hypersensitivity response to antigen rechallenge and the decreased IFN-gamma secretion by T cells isolated from the draining lymph nodes to these rechallenged areas demonstrate a defective Th1 response
• defective class switching of antigen-specific immunoglobulin to IgG2a gives further evidence of a defective Th1-response
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 04/16/2024 MGI 6.23 |
|
|
|
||