Phenotypes associated with this allele

• Allele Symbol: Dll4^tm1Jrt
• Allele Name: targeted mutation 1, Janet Rossant
• Allele ID: MGI:3056400
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dll4^tm1Jrt/Dll4^tm1Jrt	involves: 129S1/Sv * 129X1/SvJ * ICR	MGI:3056405
ht2	Dll4^tm1Jrt/Dll4^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3056439
ht3	Dll4^tm1Jrt/Dll4^+	involves: 129S1/Sv * 129X1/SvJ * ICR	MGI:3056438

Genotype
MGI:3056405

hm1

• Allelic Composition: Dll4^tm1Jrt/Dll4^tm1Jrt
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * ICR

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:93157 )

• by E10.5 no viable embryos are found

cardiovascular system

abnormal artery morphology ( J:93157 )

• the major arteries are abnormal

• endothelial cells accumulate at the apical portion of the intersomitic vessels and the dorsal vessel in this region is dilated

• arterial atrophy is seen by E9.5

abnormal vascular development ( J:93157 )

• many arteries no longer develop as arteries but show markers of a venous fate

abnormal dorsal aorta morphology ( J:93157 )

• the diameter of the dorsal aorta is reduced by E8.75

• by E9.5 the dorsal aorta is severely reduced or absent

abnormal vascular regression ( J:93157 )

• the vasculature in the head remains a simple plexus of disorganized and fused vessels

abnormal vascular branching morphogenesis ( J:93157 )

• branching morphogenesis is reduced

abnormal anterior cardinal vein morphology ( J:93157 )

• in some embryos the dorsal aorta and anterior cardinal vein are fused at the level of the sinus venosus

abnormal vein morphology ( J:93157 )

• at E8.75 the anterior cardinal vein is reduced in caliber and has ectopic branches, at E9.0 the reduction in size is more severe, and by E9.5 a distinct anterior cardinal vein is absent

abnormal sinus venosus morphology ( J:93157 )

• at E8.75 the sinus venosus is smaller than normal and this reduction is more severe at E 9.5

abnormal heart atrium morphology ( J:93157 )

• the atria are reduced is size

abnormal heart ventricle morphology ( J:93157 )

• the ventricles are reduced in size

trabecula carnea hypoplasia ( J:93157 )

pericardial edema ( J:93157 )

• severe pericardial swelling is seen at E9.0

embryo

embryonic growth retardation ( J:93157 )

• severe growth retardation is seen by E9.5

abnormal vitelline vascular remodeling ( J:93157 )

• the major vitelline artery is severely reduced

• the vascular plexus is not remodeled into a more mature vascular network

muscle

trabecula carnea hypoplasia ( J:93157 )

growth/size/body

embryonic growth retardation ( J:93157 )

• severe growth retardation is seen by E9.5

homeostasis/metabolism

pericardial edema ( J:93157 )

• severe pericardial swelling is seen at E9.0

cellular

abnormal vascular regression ( J:93157 )

• the vasculature in the head remains a simple plexus of disorganized and fused vessels

Genotype
MGI:3056439

ht2

• Allelic Composition: Dll4^tm1Jrt/Dll4⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:93157 )

• total embryonic lethality is seen after E10.5 when chimeras are crossed to 129S1/Sv females

Genotype
MGI:3056438

ht3

• Allelic Composition: Dll4^tm1Jrt/Dll4⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * ICR

mortality/aging

prenatal lethality, incomplete penetrance ( J:93157 )

• partial lethality is seen after E10.5 when chimeras are crossed to ICR females

cardiovascular system

abnormal dorsal aorta morphology ( J:93157 )

• the diameter of the dorsal aorta is reduced in 60% of embryos at E9.0 and in 90% of embryos at E9.5 and E10.5

• the location and extent of constriction varied, but was primarily observed rostrally between the cardiac level and the intersections with the branchial arch arteries, but some embryos exhibited longer zones of constriction extending caudally

abnormal umbilical artery morphology ( J:93157 )

• the umbilical artery is reduced in size

abnormal placenta vasculature ( J:93157 )

• the placental blood vessels are reduced in size

abnormal cardinal vein morphology ( J:93157 )

• the anterior and posterior cardinal veins were reduced in caliber and disorganized in embryos with severely constricted dorsal aortas

abnormal vitelline vasculature morphology ( J:93157 )

• the major vitelline arteries and arterial branching on the yolk sac are reduced in all embryos

abnormal pericardial cavity morphology ( J:93157 )

• the pericardial space is enlarged in more severely affected embryos at E10.5

poor circulation ( J:93157 )

• all embryos show reduced vitelline circulation

embryo

abnormal umbilical artery morphology ( J:93157 )

• the umbilical artery is reduced in size

abnormal placenta vasculature ( J:93157 )

• the placental blood vessels are reduced in size

abnormal vitelline vasculature morphology ( J:93157 )

• the major vitelline arteries and arterial branching on the yolk sac are reduced in all embryos

embryonic growth retardation ( J:93157 )

• a progressive developmental delay is seen in some embryos starting at E8.5

growth/size/body

embryonic growth retardation ( J:93157 )

• a progressive developmental delay is seen in some embryos starting at E8.5