Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-OVAL)916Jen
• Allele Name: transgene insertion 916, Marc K Jenkins
• Allele ID: MGI:3050829
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(CAG-OVAL)916Jen/?	C57BL/6-Tg(CAG-OVAL)916Jen	MGI:3789191

Genotype
MGI:3789191

tg1

• Allelic Composition: Tg(CAG-OVAL)916Jen/?
• Genetic Background: C57BL/6-Tg(CAG-OVAL)916Jen

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell morphology ( J:91742 )

• most cells of the body express a membrane bound form of chicken ovalbumin on their cell surface

immune system

abnormal dendritic cell antigen presentation ( J:91742 )

• dendritic cells in the skin present an epitope of chicken ovalbumin in complex with the MHC allele K^b

• splenocytes are able to activate OT-I and OT-II transgenic T cells when cultured in vitro

decreased length of allograft survival ( J:91742 )

• full thickness tail skin grafted from transgenic mice to C57BL/6 mice have a mean survival time of 33.9 days compared a mean survival time of over 200 days for non-transgenic donors

• recipient mice show accelerated rejection (18 days) when grafted with transgenic tail skin a second time

• recipient mice also show accelerated rejection when adoptively transferred with OT-I or OT-II T cells (OT-I = Tg(TcraTcrb)1100Mjb, OT-II = Tg(TcraTcrb)425Cbn)

• when recipient mice lack T cells (Tcra^tm1Mom homozygotes), rejection of the transgenic skin grafts fail to occur

• when recipient mice lack CD8 T cells (Cd8a^tm1Mak homozygotes), transgenic skin grafts shrink rapidly to 10-30% of their original size but then survive indefinitely

• when recipient mice lack CD4 T cells (H2-Ab1^tm1Gru homozygotes), transgenic skin grafts remain healthy for the first 40 days after which they start progressively contracting with 25% of the grafts being lost by 115 days