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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nkx2-5tm1Krc
targeted mutation 1, Kenneth R Chien
MGI:3042511
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Myl2tm1(cre)Krc/Myl2+
Nkx2-5tm1Krc/Nkx2-5tm1Krc
either: (involves: 129/Sv * Black Swiss) or (involves: 129/Sv * C57BL/6) MGI:3043007
cn2
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Tg(Mef2c-cre)#Blk/0
involves: 129 * C57BL/6 MGI:5643690
cn3
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Isl1tm1(cre)Sev/Isl1+
involves: 129 * C57BL/6 MGI:5643691
cn4
Foxa2tm2.1(cre/Esr1*)Moon/Foxa2+
Nkx2-5tm1Krc/Nkx2-5tm1Krc
involves: 129 * C57BL/6 MGI:5643695
cn5
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Isl1tm1(cre)Tmj/Isl1+
involves: 129X1/SvJ * C57BL/6 MGI:5643689


Genotype
MGI:3043007
cn1
Allelic
Composition
Myl2tm1(cre)Krc/Myl2+
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Genetic
Background
either: (involves: 129/Sv * Black Swiss) or (involves: 129/Sv * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myl2tm1(cre)Krc mutation (2 available); any Myl2 mutation (11 available)
Nkx2-5tm1Krc mutation (0 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• incomplete penetrance; mice were born at 78% of the expected rate
• pernatal lethality was reported to be more penetrant with contributions from the C57BL/6 genetic background

cardiovascular system
N
• no atrial or ventricular septal defects
• atrioventricular (AV) node and bundle of His were hypoplastic at birth
• atrioventricular (AV) node and bundle of His were hypoplastic at birth
• loss of integrity and sarcomeric structure in conduction system cardiomyocytes
• surrounding, non-conducting, cardiomyoctes were unaffected
• hypertrabeculation; trabecular muscle overgrowth, filling the left ventricle
• progressive AV block associated with cardiomyocyte dropout and fibrosis in the central conduction system

homeostasis/metabolism
• fetuses that died prior to birth were edematous, suggesting circulatory failure

muscle
• atrioventricular (AV) node and bundle of His were hypoplastic at birth
• atrioventricular (AV) node and bundle of His were hypoplastic at birth
• loss of integrity and sarcomeric structure in conduction system cardiomyocytes
• surrounding, non-conducting, cardiomyoctes were unaffected
• hypertrabeculation; trabecular muscle overgrowth, filling the left ventricle




Genotype
MGI:5643690
cn2
Allelic
Composition
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Tg(Mef2c-cre)#Blk/0
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm1Krc mutation (0 available); any Nkx2-5 mutation (14 available)
Tg(Mef2c-cre)#Blk mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• early embryonic lethality at E10-E10.5

cardiovascular system
• foreshortened outflow tract
• loss of right ventricle




Genotype
MGI:5643691
cn3
Allelic
Composition
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Isl1tm1(cre)Sev/Isl1+
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Isl1tm1(cre)Sev mutation (1 available); any Isl1 mutation (21 available)
Nkx2-5tm1Krc mutation (0 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• early embryonic lethality at E10-E10.5

cardiovascular system
• foreshortened outflow tract
• treatment of pregnant females from E8 to E10 with LiCl results in septation of the outflow tract in embryos, although it fails to correct the alignment defects of the outflow tract
• loss of right ventricle
• treatment of pregnant females from E8 to E10 with LiCl results in a larger right ventricle




Genotype
MGI:5643695
cn4
Allelic
Composition
Foxa2tm2.1(cre/Esr1*)Moon/Foxa2+
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm2.1(cre/Esr1*)Moon mutation (1 available); any Foxa2 mutation (21 available)
Nkx2-5tm1Krc mutation (0 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• pups from pregnant females treated with tamoxifen at E6.5-7 do not exhibit outflow tract septation or alignment defects




Genotype
MGI:5643689
cn5
Allelic
Composition
Nkx2-5tm1Krc/Nkx2-5tm1Krc
Isl1tm1(cre)Tmj/Isl1+
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Isl1tm1(cre)Tmj mutation (0 available); any Isl1 mutation (21 available)
Nkx2-5tm1Krc mutation (0 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• cyanosis and death within a few hours of birth; perinatal lethality is due to outflow tract septation defects

cardiovascular system
• the trabecular myocardium, but not the compact myocardium, shows a reduction in the relative proliferation rate in embryos
• foreshortened outflow tract
• complete penetrance of outflow tract defects
• the proximal outflow tract shows defective endocardial networks, while the neural crest-derived distal outflow tract endocardial cushions appear normal
• smaller developing endocardial cushions in the proximal outflow tract
• the outflow tract cushions show a reduction in the relative proliferation rate in embryos
• 92% of mutants exhibit a single outflow tract arising from the right ventricle
• only a small number of embryos show double outlet right ventricle
• reduction of trabecular networks in the right ventricle, whereas the left ventricle is normal
• decrease in the relative proliferative rate of the second heart field is evident as early as E10, resulting in hypoplastic right ventricle at E12.5

homeostasis/metabolism

muscle
• the trabecular myocardium, but not the compact myocardium, shows a reduction in the relative proliferation rate in embryos





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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory