• homozygotes are viable, fertile, grow at a normal rate, and show no histological or physiological abnormalities at >1 year of age

• no effect on retinal ganglion cell survival after optic nerve axotomy (J:173661)

• newborn mice exposed to doxycycline from E14.5 to birth display defective distal lung saccular development due to reduced primary septation

• newborn mice exposed to doxycycline from E14.5 to birth display dilated distal airspaces with only few primary septae, unlike wild-type controls where extensive numbers of saccules are observed

• ovarian surface epithelium cells transfected with a cre-expressing adenovirus exhibit reduced invasion compared with cells from Trp53^tm1Brn homozygotes transfected with a lacZ-expressing adenoviru (J:175978)

• mice exhibit reduced motor axon arborization (length and number of branches) in the pectoralis minor muscle compared with wild-type mice

• at P2, endplates in the pectoralis minor exhibit increased partially or completely denervated synapses compared with wild-type endplates

• however, mice exhibit normal innervation of the pectoralis minor muscle at E15.5 and of the pectoralis major

• by P2 in the C8 and T1 region of the spinal cord with no further reduction in adult mice

• mice exhibit reduced motor axon arborization (length and number of branches) in the pectoralis minor muscle compared with wild-type mice

• at P2, endplates in the pectoralis minor exhibit increased partially or completely denervated synapses compared with wild-type endplates

• however, mice exhibit normal innervation of the pectoralis minor muscle at E15.5 and of the pectoralis major

• in a wire hang test but not a rotarod

• ovarian surface epithelium cells transfected with a cre-expressing adenovirus exhibit reduced invasion compared with similarly treated cells from Trp53^tm1Brn/Trp53^tm3Tyj mice (J:175978)

• ovarian surface epithelium cells transfected with a cre-expressing adenovirus exhibit reduced invasion compared with similarly treated cells from Trp53^tm1Brn/Trp53^tm2Tyj mice (J:175978)

• mutants develop hemorrhagic liver destruction within the first few hours in response to apoptotic stimuli induced by Fas (Tnfrsf6) antibody compared to only minor liver damage in controls

• primary hepatocytes show reduced phagocytic activity towards bacteria (E.coli) than control cells

• hepatocytes exhibit increased sensitivity to Fas (Tnfrsf6)-induced apoptosis

• the ability of mutant livers to clear necrotic tissue and to repopulate the injured (by CCl₄ exposure) area by regenerating hepatocytes is impaired

• associated with a persistent inflammatory reaction, overproduction of osteopontin, early and prominent dystrophic calcification, and impaired hepatocyte scattering/migration into diseased areas

• 80% of mutants die of acute liver failure in response to apoptotic stimuli induced by Fas (Tnfrsf6) antibody while controls survive

• mutants develop hemorrhagic liver destruction within the first few hours in response to apoptotic stimuli induced by Fas (Tnfrsf6) antibody compared to only minor liver damage in controls

• mutants exhibit delayed healing after toxic liver injury induced by CCl₄ exposure

• mutants are more vulnerable to hepatectomy surgery and most are either moribund or die by 48 hours after surgery

• hepatocytes fail to migrate in a standard wound healing assay

• hepatocytes exhibit increased sensitivity to Fas (Tnfrsf6)-induced apoptosis

• by P2 with no further reduction in adult mice

• lacZ+ motor neurons are reduced at P2 in C8 and T1 regions of the spinal cord

• in the Morris water maze, mutants have a longer latency to locate the platform on the first 2 days of training than controls, however performance on the probe test and reversal probe test were normal, indicating normal hippocampal-mediated spatial learning (J:170554)

• open field activity and anxiety are not affected (J:170554)

• on reversal discriminations, mutants require more trials than controls, indicating impaired reversal learning

• mutants show increased latency performance on the cued-platform test, indicating a delay in striatal-dependent cued learning

• marker analysis indicates an increase in numbers of PV+ and SST+ striatal GABAergic interneurons

• distribution of striatal interneurons is altered, with fewer cells of the population located in the medial (associative) regions and more cells in the lateral (sensorimotor) areas

• reduction in the numbers of PV+ GABAergic interneurons in the primary somatosensory cortex (28% loss) and the orbitofrontal cortex (31% loss)

• marker analysis indicates an increase in numbers of PV+ and SST+ striatal GABAergic interneurons

• however, striatum appears normal

• 31% reduction in PV+ cells in the orbitofrontal cortex

• 28% loss of PV+ cells in primary somatosensory cortex