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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(Cryaa-cre)10Mlr
transgene insertion 10, Mike Robinson
MGI:3038243
Summary 16 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ctnnb1tm4Wbm/Ctnnb1tm4Wbm
Tg(Cryaa-cre)10Mlr/?
involves: 129P2/OlaHsd * FVB/N MGI:3811549
cn2
Gt(ROSA)26Sortm1(Rybp/EGFP)Cve/Gt(ROSA)26Sor+
Tg(Cryaa-cre)10Mlr/0
involves: 129S1/Sv * 129X1/SvJ MGI:3805333
cn3
Dlg1tm1.1Agri/Dlg1tm1.1Agri
Tg(Cryaa-cre)10Mlr/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N MGI:4354993
cn4
Pax6tm2Pgr/Pax6tm2Pgr
Sox2tm1.1Lan/Sox2tm1.1Lan
Tg(Cryaa-cre)10Mlr/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL MGI:4366457
cn5
Pax6tm2Pgr/Pax6tm2Pgr
Tg(Cryaa-cre)10Mlr/0
involves: 129S1/Sv * 129X1/SvJ * FVB/N MGI:4366458
cn6
Gclctm1c(EUCOMM)Wtsi/Gclctm1c(EUCOMM)Wtsi
Tg(Cryaa-cre)10Mlr/0
involves: 129S4/SvJaeSor * C57BL/6N * FVB/N MGI:5475286
cn7
Gclctm1c(EUCOMM)Wtsi/Gclc+
Tg(Cryaa-cre)10Mlr/0
involves: 129S4/SvJaeSor * C57BL/6N * FVB/N MGI:5475288
cn8
Cdk1tm1Eddy/Cdk1tm1Eddy
Tg(Cryaa-cre)10Mlr/0
involves: 129S6/SvEvTac * C57BL/6NCrl * FVB/N MGI:5604427
cn9
Atg5tm1Myok/Atg5tm1Myok
Tg(CAG-EGFP/Map1lc3b)53Nmz/0
Tg(Cryaa-cre)10Mlr/0
involves: 129S/SvEv * C57BL/6 * DBA/2 * FVB/N MGI:5529811
cn10
Itgb1tm1Efu/Itgb1tm1Efu
Tg(Cryaa-cre)10Mlr/?
involves: 129X1/SvJ * C57BL/6 MGI:3715212
cn11
Ctnnb1tm1Mmt/Ctnnb1tm1Mmt
Tg(Cryaa-cre)10Mlr/0
involves: 129X1/SvJ * FVB/N MGI:4366459
cn12
Stat3tm1Dlv/Stat3tm1Dlv
Tg(Cryaa-cre)10Mlr/0
involves: C57BL/6 MGI:3038248
cn13
Sox2tm1.1Lan/Sox2tm1.1Lan
Tg(Cryaa-cre)10Mlr/0
involves: C57BL/6 * FVB/N * SJL MGI:4366456
cn14
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Cryaa-cre)10Mlr/?
involves: FVB/N MGI:4417871
cn15
Prkcitm1Kido/Prkcitm1Kido
Tg(Cryaa-cre)10Mlr/?
involves: FVB/N MGI:4417872
cn16
Pik3c3tm1Tssk/Pik3c3tm1Tssk
Tg(Cryaa-cre)10Mlr/0
involves: FVB/N MGI:5529810


Genotype
MGI:3811549
cn1
Allelic
Composition
Ctnnb1tm4Wbm/Ctnnb1tm4Wbm
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: 129P2/OlaHsd * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1tm4Wbm mutation (1 available); any Ctnnb1 mutation (41 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at E14.5, the lens capsule is thinner than in controls
• the anterior capsule is extremely thin, whereas the posterior capsule is abnormally thick and diffuse at 1 day of age
• the close adherence of the iris to the remaining abnormal epithelial cells is further evidence of an abnormal lens capsule at 1 day of age
• at 21 days of age, abnormal fiber cells are surrounded by a very thin basement membrane that is often ruptured resulting in fiber cell extrusion into the vitreous and posterior chamber of the aqueous near the iris
• the cell cycle of lens epithelial cells is disrupted by E13.5 with 3-fold less cells being in the S phase or M phase
• at E14.5, epithelium is thin with the presence of blood cells found under an abnormal multilayered epithelium
• at P1, the lens epithelium is thin and the epithelial cells abnormally shaped
• at P21, the residual lens has no distinguishable epithelium and a thin basement membrane
• at E14.5, fiber cells displayed altered apical?basal polarity as evidenced by altered distribution of the tight junction protein, ZO1, disruption of apical actin filaments, and accumulations of extracellular matrix containing collagen
• at P1, fiber cells are poorly elongated and tend to be aligned more perpendicularly to the capsule than parallel
• at P21, the abnormal fiber cells are often extruding into the vitreous
• small lens size is discernable at 1 day of age and size is greatly reduced by 21 days of age
• small eye phenotype is discernable at eye opening at 12 days of age with greatly reduced eye size evident by weaning

integument
• occurs in patches on the cheek by 21 days of age
• occurs by 21 days of age




Genotype
MGI:3805333
cn2
Allelic
Composition
Gt(ROSA)26Sortm1(Rybp/EGFP)Cve/Gt(ROSA)26Sor+
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(Rybp/EGFP)Cve mutation (0 available); any Gt(ROSA)26Sor mutation (764 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• lens from 2 day old mice exhibit cortical inhomogenicity
• lens fiber mass decreases with age
• cataracts develop in adult mice due to loss of lens fiber cells




Genotype
MGI:4354993
cn3
Allelic
Composition
Dlg1tm1.1Agri/Dlg1tm1.1Agri
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dlg1tm1.1Agri mutation (0 available); any Dlg1 mutation (60 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at P2, levels of apoptosis in the epithelium are increased compared to in wild-type mice
• in the anterior, posterior, and center of the lens
• lenses exhibit an abnormal shape with shortened anterior-posterior dimension
• denucleation of the fiber cell compartment is delayed compared to in wild-type mice
• anterior and posterior suture formation is defective
• at E13.5, lens epithelium is thickened and contains disorganized areas unlike in wild-type mice due to increased cell numbers
• at P2, the integrity of the lens epithelium is severely disrupted compared to in wild-type mice
• the central epithelium in lenses appears flattened and the uniform positioning of nuclei within cells is lost unlike in wild-type mice
• lens contain loosely packed cells, clusters of nuclei followed by gaps in the row of nuclei and vacuoles along with a few areas of multilayering in the peripheral epithelium unlike in wild-type mice
• however, at P2 the number of epithelial cells is normal
• at P24, a few areas of multilayering and irregular nuclei shapes are still observed in the peripheral epithelium and the central epithelium remained flattened unlike in wild-type mice
• in some mice fiber cells fail to exhibit convex curvature and in other cases have an irregular curvature unlike in wild-type mice
• differentiation of fiber cells is disrupted

cellular
• at P2, levels of apoptosis in the epithelium are increased compared to in wild-type mice
• in the anterior, posterior, and center of the lens




Genotype
MGI:4366457
cn4
Allelic
Composition
Pax6tm2Pgr/Pax6tm2Pgr
Sox2tm1.1Lan/Sox2tm1.1Lan
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax6tm2Pgr mutation (1 available); any Pax6 mutation (73 available)
Sox2tm1.1Lan mutation (1 available); any Sox2 mutation (47 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• lens epithelial cells accumulate posterior to the lens equator unlike in wild-type mice
• anterior lens epithelium is reduced in size compared to in wild-type mice
• lens epithelial cells fail to exhibit the cell cycle

cellular




Genotype
MGI:4366458
cn5
Allelic
Composition
Pax6tm2Pgr/Pax6tm2Pgr
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax6tm2Pgr mutation (1 available); any Pax6 mutation (73 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• thickened at E14.5
• lens tissue is reduced, opaque, and shapeless compared to in wild-type mice
• at E14.5, the lens is slightly more elongated than in wild-type mice
• at E15.5, transitional zone cells are disorganized unlike in wild-type mice
• anterior lens epithelial cells fail to exit the cell cycle at the equator unlike in wild-type mice
• anterior lens epithelial cells fail to differentiate and accumulate at the lens equator and in the posterior lens unlike in wild-type mice
• anterior lens epithelial cells fail to exit the cell cycle at the equator unlike in wild-type mice
• anterior lens epithelial cells fail to differentiate and accumulate at the lens equator and in the posterior lens unlike in wild-type mice
• lens fiber formation is arrested from E14.5 unlike in wild-type mice
• at P4, few fiber cells are found in the lenses unlike in wild-type mice
• at P30, no lens fiber cells are detected unlike in wild-type mice
• at E14.5, mice exhibit small lenses compared to in wild-type mice
• lens tissue is decreased compared to in wild-type mice
• 65% smaller by circumference compared with wild-type eyes

cellular




Genotype
MGI:5475286
cn6
Allelic
Composition
Gclctm1c(EUCOMM)Wtsi/Gclctm1c(EUCOMM)Wtsi
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6N * FVB/N
Cell Lines EPD0051_3_D07
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gclctm1c(EUCOMM)Wtsi mutation (0 available); any Gclc mutation (13 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Cataracts in Gclctm1c(EUCOMM)Wtsi/Gclctm1c(EUCOMM)Wtsi Tg(Cryaa-cre)10Mlr/0 mice

vision/eye
• in some mice starting at 3 months
• mature cataracts in half of mice by 4 months
• small at 4 months
• severe at 9 months

cellular
• strong increase in reactive oxygen species production as determined by GSSG/GSH ratio

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
cataract DOID:83 OMIM:601371
OMIM:PS116200
J:194778




Genotype
MGI:5475288
cn7
Allelic
Composition
Gclctm1c(EUCOMM)Wtsi/Gclc+
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6N * FVB/N
Cell Lines EPD0051_3_D07
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gclctm1c(EUCOMM)Wtsi mutation (0 available); any Gclc mutation (13 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• mild increase in reactive oxygen species production as determined by GSSG/GSH ratio




Genotype
MGI:5604427
cn8
Allelic
Composition
Cdk1tm1Eddy/Cdk1tm1Eddy
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6NCrl * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdk1tm1Eddy mutation (1 available); any Cdk1 mutation (18 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• from E17.5, fewer cells appear to undergo secondary fiber cell differentiation
• epithelial cells in lenses fail to undergo mitosis and exhibit DNA endoreduplication by E15.5-E17.5
• a reduced number of lens epithelial cells and the increase in nuclear size at E15.5-E17.5 suggest that cells bypass mitosis and undergo endoreduplication of their DNA during the cell cycle
• by E15.5, the nuclei in the differentiating secondary fibers at the bow region of lenses appears 152% larger compared to controls
• E17.5 lenses retain nuclei in the center of the lens, resulting in a failure to form an organelle-free zone
• fewer fiber cell nuclei are seen in various zones of the lens




Genotype
MGI:5529811
cn9
Allelic
Composition
Atg5tm1Myok/Atg5tm1Myok
Tg(CAG-EGFP/Map1lc3b)53Nmz/0
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129S/SvEv * C57BL/6 * DBA/2 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atg5tm1Myok mutation (2 available); any Atg5 mutation (27 available)
Tg(CAG-EGFP/Map1lc3b)53Nmz mutation (4 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Cataract development in Atg5tm1Myok/Atg5tm1Myok Tg(CAG-EGFP/Map1lc3b)53Nmz/0 Tg(Cryaa-cre)10Mlr/0 mice

homeostasis/metabolism
• absent in lens fiber cells

vision/eye
• absent autophagy in lens fiber cells
• disorganized in the cortical region of aged mice
• however, organelle degradation is normal
• by 6 to 9 months that develops progressively with age
• severe, bilateral at 21 months
• with accumulation of insoluble oxidized proteins and crystallins

cellular
• absent in lens fiber cells




Genotype
MGI:3715212
cn10
Allelic
Composition
Itgb1tm1Efu/Itgb1tm1Efu
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb1tm1Efu mutation (2 available); any Itgb1 mutation (42 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at E16.5, mild lens epithelial elongation is observed
• at birth, lens epithelium is missing and remaining epithelial cells are abnormally elongated
• at birth, epithelial cells in the lens are lost by apoptosis
• at birth, lens fibers are highly vacuolated and disintegrating
• adult mice have little to no lens material
• microphthalmia in adults
• however, mice are born with normal sized eyes




Genotype
MGI:4366459
cn11
Allelic
Composition
Ctnnb1tm1Mmt/Ctnnb1tm1Mmt
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1tm1Mmt mutation (0 available); any Ctnnb1 mutation (41 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• at E15.5, lens epithelial cells accumulate posterior to the lens equator unlike in wild-type mice
• lens epithelial cells fail to exhibit the cell cycle

cellular




Genotype
MGI:3038248
cn12
Allelic
Composition
Stat3tm1Dlv/Stat3tm1Dlv
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat3tm1Dlv mutation (0 available); any Stat3 mutation (61 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
N
• lens development was normal




Genotype
MGI:4366456
cn13
Allelic
Composition
Sox2tm1.1Lan/Sox2tm1.1Lan
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: C57BL/6 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox2tm1.1Lan mutation (1 available); any Sox2 mutation (47 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
N
• mice have normal eyes




Genotype
MGI:4417871
cn14
Allelic
Composition
Prkcitm1Kido/Prkcitm1.1Kido
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1.1Kido mutation (0 available); any Prkci mutation (70 available)
Prkcitm1Kido mutation (0 available); any Prkci mutation (70 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• pigment cells cover the surface of the lens
• small opaque pupils speckled with black material
• lenses are still normal at E10.5
• lens vesicle closes normally at E11.5
• lens epithelium layer thinner at E18.5 than for controls
• reduced cell proliferation in germinative zone of the lens epithelium
• increased expression of alpha smooth muscle actin in lens epithelia after E16.5
• fiber cell alignment severely disrupted at E18.5
• youngest lens fiber cells have lost apical connections
• interface between lens epithelial cells and fiber cells is much shorter at E12.5
• small almond shaped eyes
• smaller eyeball

pigmentation
• pigment cells cover the surface of the lens




Genotype
MGI:4417872
cn15
Allelic
Composition
Prkcitm1Kido/Prkcitm1Kido
Tg(Cryaa-cre)10Mlr/?
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcitm1Kido mutation (0 available); any Prkci mutation (70 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• pigment cells cover the surface of the lens
• small opaque pupils speckled with black material
• lenses are still normal at E10.5
• lens vesicle closes normally at E11.5
• lens epithelium layer thinner at E18.5 than for controls
• reduced cell proliferation in germinative zone of the lens epithelium
• increased expression of alpha smooth muscle actin in lens epithelia after E16.5
• fiber cell alignment severely disrupted at E18.5
• youngest lens fiber cells have lost apical connections
• interface between lens epithelial cells and fiber cells is much shorter at E12.5
• small almond shaped eyes
• smaller eyeball

pigmentation
• pigment cells cover the surface of the lens




Genotype
MGI:5529810
cn16
Allelic
Composition
Pik3c3tm1Tssk/Pik3c3tm1Tssk
Tg(Cryaa-cre)10Mlr/0
Genetic
Background
involves: FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3c3tm1Tssk mutation (0 available); any Pik3c3 mutation (35 available)
Tg(Cryaa-cre)10Mlr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Cataracts and impaired differentiation of secondary lens fiber cells in Pik3c3tm1Tssk/Pik3c3tm1Tssk Tg(Cryaa-cre)10Mlr/0 mice

homeostasis/metabolism
• inhibited autophagy in the lens at E16

vision/eye
• inhibited autophagy in the lens at E16
• impaired differentiation of secondary lens fiber cells with accumulation of vacuoles
• however, organelle degradation is normal
• bilateral at P0.5
• at P0.5, P7.5 and 2 months
• at birth and more pronounced at 2 months

cellular
• inhibited autophagy in the lens at E16





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last database update
06/23/2022
MGI 6.20
The Jackson Laboratory