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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nkx3-2tm1Tlu
targeted mutation 1, Thomas Lufkin
MGI:3032961
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Nkx3-2tm1Tlu/Nkx3-2tm1Tlu either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) MGI:3719254
ht2
Nkx3-2tm1Tlu/Nkx3-2+ either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) MGI:3840655


Genotype
MGI:3719254
hm1
Allelic
Composition
Nkx3-2tm1Tlu/Nkx3-2tm1Tlu
Genetic
Background
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx3-2tm1Tlu mutation (0 available); any Nkx3-2 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• newborns die within minutes of birth, probably because they fail to initiate normal respiration

growth/size/body
• thoracic cavity is distended radially
• slightly shorter in stature

hematopoietic system
• 100% penetrance of asplenia in newborns; earliest absence of spleen precursor cells is seen at E11.5, at the time of normal appearance of spleen anlage

immune system
• 100% penetrance of asplenia in newborns; earliest absence of spleen precursor cells is seen at E11.5, at the time of normal appearance of spleen anlage

craniofacial
• floor of the cranial vault is either reduced in size or has severe dysmorphology of the basisphenoid and basioccipital bones
• exoccipital bone is reduced in size and displays fissures

limbs/digits/tail
N
• no defects in the limb skeleton
• truncated tails

skeleton
• floor of the cranial vault is either reduced in size or has severe dysmorphology of the basisphenoid and basioccipital bones
• exoccipital bone is reduced in size and displays fissures
• intervertebral discs are about 0.2-0.5 times the normal thickness
• absent anterior arch of atlas
• neural arches are about 0.2-0.5 times the normal thickness
• in all vertebrae, the vertebral bodies are hypoplastic, with only a small region of the dorsal vertebral body still present and the ventral vertebral body completely absent
• all vertebral bodies at E18.5 show a total loss of ossification centers and at E12.5, chondrogenic condensations surrounding the notochord do not exhibit ossification centers
• 2.7-fold increase in the number of cells undergoing apoptosis in developing vertebral bodies at E10.5-E14.5
• reduction in the overall rostrocaudal length of individual vertebrae
• reduction in length of the vertebral column
• chondrogenesis is absent in vertebral bodies at E12.5 and E14.5
• all vertebral bodies at E18.5 show a total loss of ossification centers
• total absence of the ossification center of the cartilage primordium of the body of the hyoid bone




Genotype
MGI:3840655
ht2
Allelic
Composition
Nkx3-2tm1Tlu/Nkx3-2+
Genetic
Background
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx3-2tm1Tlu mutation (0 available); any Nkx3-2 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
skeleton
• 78% of mutants exhibit mild abnormalities of the vertebrae, such as split or reduced ossification centers
• however, overall length of the vertebral column and neural arch morphology are not affected





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory