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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ptk2tm1Lfr
targeted mutation 1, Louis F Reichardt
MGI:2684666
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ptk2tm1Lfr/Ptk2tm1Ilic
Tg(KRT5-cre)5132Jlj/0
involves: 129X1/SvJ * C57BL/6 * CBA * DBA/2J MGI:3625475
cn2
Ptk2tm1Lfr/Ptk2tm1Lfr
Tg(Tek-cre)1Rwng/0
involves: 129X1/SvJ * FVB/N MGI:3689988
cx3
Fyntm1Yik/Fyntm1Yik
Ptk2tm1Lfr/Ptk2+
involves: 129X1/SvJ * C57BL/6 * CBA MGI:3693516


Genotype
MGI:3625475
cn1
Allelic
Composition
Ptk2tm1Lfr/Ptk2tm1Ilic
Tg(KRT5-cre)5132Jlj/0
Genetic
Background
involves: 129X1/SvJ * C57BL/6 * CBA * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptk2tm1Ilic mutation (0 available); any Ptk2 mutation (90 available)
Ptk2tm1Lfr mutation (1 available); any Ptk2 mutation (90 available)
Tg(KRT5-cre)5132Jlj mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• a significant deficit in sebaceous glands in 2-4 month-old mice

integument
• primary keratinocytes isolated from mutant animals would not proliferate in culture
• a significant deficit in sebaceous glands in 2-4 month-old mice
• from approximately postnatal day 7 until P17, initial hair growth was sparse
• by P17, the pelage of mutant mice appeared identical to their normal littermates
• hair follicles are orientated randomly, rather than being arrayed in a hexagonal pattern as in control in P12 mice
• have consistently about 25% fewer hair follicles than control
• irregular hair cycle suggested by the presence of hair follicles in the hypodermis of P22 mice
• adult mutant mice periodically showed patches of receding hair, which lasted for several days, disappeared, and then appeared again at another location
• a thinner epidermis was evident in mutant mice at all ages

cellular
• primary keratinocytes isolated from mutant animals would not proliferate in culture




Genotype
MGI:3689988
cn2
Allelic
Composition
Ptk2tm1Lfr/Ptk2tm1Lfr
Tg(Tek-cre)1Rwng/0
Genetic
Background
involves: 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptk2tm1Lfr mutation (1 available); any Ptk2 mutation (90 available)
Tg(Tek-cre)1Rwng mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

growth/size/body
• slightly smaller at E10.5

embryo
• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5
• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels
• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections
• slightly smaller at E10.5

cardiovascular system
• show patches of sequestered blood, reflecting dilated vessels, primarily in the upper trunk and head regions
• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries
• para-aortic splanchnopleural mesoderm explants undergo a process in which endothelial cells contract and cluster with each other, resulting in an irregular network composed of wider and smaller vessels
• isolated endothelial cells grown on fibronectin are poorly spread, lack membrane ruffling and lamellipodia formation and show aberrant locomotion that is faster in the presence of serum and growth supplements
• endothelial cells show fewer and abnormal stress fibers that instead resemble cortical actin bundles and show fewer focal adhesions
• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries, leading to a great variation in the size of the capillaries and intercapillary spaces
• allantoic explants and para-aortic splanchnopleural mesoderm explants show dilation of capillaries and intercapillary spaces with a reduction in network complexity
• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression
• exhibit complete absence of blood vessels in the neuroepithelium at E10.5, indicating defective sprouting angiogenesis into the neuroepithelium
• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5
• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels
• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections
• hemorrhage is apparent in both the amniotic and yolk sac cavities

cellular
• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression




Genotype
MGI:3693516
cx3
Allelic
Composition
Fyntm1Yik/Fyntm1Yik
Ptk2tm1Lfr/Ptk2+
Genetic
Background
involves: 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fyntm1Yik mutation (1 available); any Fyn mutation (37 available)
Ptk2tm1Lfr mutation (1 available); any Ptk2 mutation (90 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• atrophy of the thymic cortex
• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age
• impaired development of CD4+CD8+ thymocytes
• exhibit a reduction in double-positive thymocytes coincident with cortical atrophy

hematopoietic system
• atrophy of the thymic cortex
• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age
• impaired development of CD4+CD8+ thymocytes
• exhibit a reduction in double-positive thymocytes coincident with cortical atrophy

endocrine/exocrine glands
• atrophy of the thymic cortex
• about 2/3 of mutants start to show a decrease in the number of thymocytes after 3 weeks of age, with numbers dropping as low as 1/100 of normal at 4 to 5 weeks of age





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory