Phenotypes associated with this allele

• Allele Symbol: Cops3^tm2Jrl
• Allele Name: targeted mutation 2, James R Lupski
• Allele ID: MGI:2678262
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cops3^tm2Jrl/Cops3^tm2Jrl	involves: 129S7/SvEvBrd * C57BL/6	MGI:2678266
ht2	Cops3^tm1Jrl/Cops3^tm2Jrl	involves: 129S7/SvEvBrd * C57BL/6	MGI:2678268

Genotype
MGI:2678266

hm1

• Allelic Composition: Cops3^tm2Jrl/Cops3^tm2Jrl
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:85760 )

• no homozygous mice are born

Genotype
MGI:2678268

ht2

• Allelic Composition: Cops3^tm1Jrl/Cops3^tm2Jrl
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:85760 )

• no homozygotes are born

• no homozygotes recovered at E9.5-11.5

• at E8.5 a single small homozygote with head anlage but lacking other structural elements found, also 7 resorbing embryos

• E7.5 1/5-1/4 normal size homozygotes and elongated shape

embryo

embryo phenotype ( J:85760 )

N • histologically normal visceral and parietal endoderm is present at E5.5 and the ectoplacental cone is forming

abnormal germ layer development ( J:85760 )

• at E6.5 and E7.5, only two germ layers are present

failure to gastrulate ( J:85760 )

• E7.5 embryos apparently arrested prior to gastrulation

embryonic growth retardation ( J:85760 )

• at 6.5 dpc, mutant embryos exhibit slight growth retardation

abnormal embryonic epiblast morphology ( J:85760 )

• at E5.5, a disorganized epiblast is seen

• at E6.5, the number of epiblast cells appeared to be greatly reduced and the cells were disorganized

• at E7.5, more epiblast cells are apparent, but cells were more disorganized

• apoptosis of epiblast cells occurs by E6.5

absent mesoderm ( J:85760 )

• no indications of mesoderm formation at E7.5

• mutant embryos showed no Brachyury expression, a marker of mesodermal cells, at E7.5

abnormal inner cell mass morphology ( J:85760 )

• inner cell mass cells from cultured blastocysts cease to develop normally after 3 days in culture

• cell numbers are greatly reduced by days 5-6 in culture

abnormal proamniotic cavity morphology ( J:85760 )

• cavity is not obvious at E5.5

• a more obvious pro-amniotic cavity is present at E6.5 that contains increased numbers of dead cells, presumably from the epiblast

growth/size/body

embryonic growth retardation ( J:85760 )

• at 6.5 dpc, mutant embryos exhibit slight growth retardation