Mouse Genome Informatics
hm1
    Pdpk1tm1.2Mlw/Pdpk1tm1.2Mlw
involves: BALB/c
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• no homozygotes found at E10 or later

cardiovascular system
• no heart tube at E9-9.5
• no heart at E9-9.5

embryogenesis
• embryos have not turned at E9-9.5
• by E7 embryos are smaller than normal
• significantly smaller in length by E8.0
• no branchial arches at E9-9.5
• E7.5 endoderm cells 40% smaller than normal
• presomitic mesoderm swollen at E9-9.5
• very small head folds at E8.0
• however, head folds are fused at E9.5
• posterior growth halted by E9-9.5
• no significant development posterior to the allantois
• somites are absent are E9.0-E9.5
• still no somites at E8.5, although a presomitic mesoderm is present
• no visible somites or posterior mesoderm at E8.0
• absence of branchial arches and lack of dorsal root ganglia indicates abnormal neural crest specification and/or migration
• small allantois at E8.0 growing larger through E9-9.5 when it comes in contact with placenta but shows no vascularization
• at E8.0

growth/size
• by E7 embryos are smaller than normal
• significantly smaller in length by E8.0
• at E8.5 the head region is less developed, resembling a small compact mass
• the head has developed normally to some extent by E9.5

nervous system
• absence of forebrain at E9.5
• however, development of the mid- and hindbrain is relatively normal
• no dorsal root ganglia at E9-9.5

craniofacial
• no branchial arches at E9-9.5

vision/eye
• absence of eyes at E9.5


Mouse Genome Informatics
ht2
    Pdpk1tm1Mlw/Pdpk1tm1.2Mlw
involves: BALB/c
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• homozygotes born at 25% lower ratio than expected

growth/size
• males about 45-50% smaller at birth
• females about 35% smaller at birth
• kidney, pancreas, spleen, adrenal gland all 50% smaller in size
• due to smaller cells