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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gucy2ctm1Gar
targeted mutation 1, David L Garbers
MGI:2665316
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Gucy2ctm1Gar/Gucy2ctm1Gar B6.129S6-Gucy2ctm1Gar MGI:5300906
hm2
Gucy2ctm1Gar/Gucy2ctm1Gar either: (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J) MGI:2665347


Genotype
MGI:5300906
hm1
Allelic
Composition
Gucy2ctm1Gar/Gucy2ctm1Gar
Genetic
Background
B6.129S6-Gucy2ctm1Gar
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gucy2ctm1Gar mutation (1 available); any Gucy2c mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
adipose tissue
• mutants exhibit an accumulation of adipose mass, including an increase in visceral and subcutaneous fat compartments

behavior/neurological
• fasted and refed mutants display deficient postprandial satiation
• mutants are hyperphagic, independent of sex, dietary nutrients, or dietary caloric content
• however, mutants exhibit normal lipid digestion, lipid absorption, distribution and clearance, normal energy mobilization and expenditure, normal circadian cycles and thermoregulatory reflexes to cold, indicating that an increase in caloric consumption is the probable cause of the excess adiposity

cardiovascular system
• mean heart size in mice raised on either the low calorie or high calorie diet is increased compared to wild-type mice

growth/size/body
• mutants raised on a standard low calorie diet, moderate calorie diet, or high calorie diet exhibit an increased body weight compared to wild-type mice
• females raised on the high calorie diet showed the highest weight increase, with mutants being 26.3% heavier than wild-type mice

homeostasis/metabolism
• fasted serum concentrations of 12 months old mutants raised on a high caloric diet are increased compared to wild-type mice on the same diet
• fasted serum leptin levels are increased at 10-12 and 18 months of age in mutants raised on either the low calorie or high calorie diet
• impaired glycemic control
• mice raised on a low calorie diet exhibit increased hepatic triglyceride content

integument

liver/biliary system
• mice raised on a low calorie diet exhibit increased hepatic triglyceride content




Genotype
MGI:2665347
hm2
Allelic
Composition
Gucy2ctm1Gar/Gucy2ctm1Gar
Genetic
Background
either: (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gucy2ctm1Gar mutation (1 available); any Gucy2c mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, most homozygotes survived to weaning, whereas all wild-type and heterozygous controls died within 72 hrs of infection
• only a small minority of homozygotes died within 24-72 hrs of administration of E. coli strain 1676; however, no diarrhea was observed and no cause of death was established
• following inoculation with a human enterotoxigenic E. coli strain (H10407) which does not colonize and infect mice, 23 of 26 homozygotes survived to weaning, while the remaining 3 died within 24 hrs of the gavage

immune system
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, homozygotes failed to develop signs of diarrhea, indicating resistance to enterotoxigenic bacteria that produce heat stable enteroxins
• following an oral inoculum of 10 U of E. coli heat-stable enterotoxin, 3-day suckling homozygotes failed to exhibit any fluid accumulation within the intestinal lumen, as determined by gut/carcass weight ratio
• in contrast, suckling homozygotes showed normal fluid accumulation within the intestine following oral inoculation with 5 ug of cholera toxin
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, most homozygotes survived to weaning, whereas all wild-type and heterozygous controls died within 72 hrs of infection
• only a small minority of homozygotes died within 24-72 hrs of administration of E. coli strain 1676; however, no diarrhea was observed and no cause of death was established
• following inoculation with a human enterotoxigenic E. coli strain (H10407) which does not colonize and infect mice, 23 of 26 homozygotes survived to weaning, while the remaining 3 died within 24 hrs of the gavage

digestive/alimentary system
• following oral inoculation with 5 ug of cholera toxin, suckling homozygotes show normal fluid accumulation within the intestine (diarrhea), as determined by gut/carcass weight ratio





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last database update
09/27/2016
MGI 6.05
The Jackson Laboratory