Mouse Genome Informatics
hm1
    Gucy2ctm1Gar/Gucy2ctm1Gar
B6.129S6-Gucy2ctm1Gar
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
adipose tissue
• mutants exhibit an accumulation of adipose mass, including an increase in visceral and subcutaneous fat compartments

behavior/neurological
• fasted and refed mutants display deficient postprandial satiation
• mutants are hyperphagic, independent of sex, dietary nutrients, or dietary caloric content
• however, mutants exhibit normal lipid digestion, lipid absorption, distribution and clearance, normal energy mobilization and expenditure, normal circadian cycles and thermoregulatory reflexes to cold, indicating that an increase in caloric consumption is the probable cause of the excess adiposity

cardiovascular system
• mean heart size in mice raised on either the low calorie or high calorie diet is increased compared to wild-type mice

growth/size
• mutants exhibit an accumulation of adipose mass, including an increase in visceral and subcutaneous fat compartments
• mutants raised on a standard low calorie diet, moderate calorie diet, or high calorie diet exhibit an increased body weight compared to wild-type mice
• females raised on the high calorie diet showed the highest weight increase, with mutants being 26.3% heavier than wild-type mice

homeostasis/metabolism
• fasted serum concentrations of 12 months old mutants raised on a high caloric diet are increased compared to wild-type mice on the same diet
• fasted serum leptin levels are increased at 10-12 and 18 months of age in mutants raised on either the low calorie or high calorie diet
• impaired glycemic control
• mice raised on a low calorie diet exhibit increased hepatic triglyceride content

integument

liver/biliary system
• mice raised on a low calorie diet exhibit increased hepatic triglyceride content


Mouse Genome Informatics
hm2
    Gucy2ctm1Gar/Gucy2ctm1Gar
either: (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J)
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, most homozygotes survived to weaning, whereas all wild-type and heterozygous controls died within 72 hrs of infection
• only a small minority of homozygotes died within 24-72 hrs of administration of E. coli strain 1676; however, no diarrhea was observed and no cause of death was established
• following inoculation with a human enterotoxigenic E. coli strain (H10407) which does not colonize and infect mice, 23 of 26 homozygotes survived to weaning, while the remaining 3 died within 24 hrs of the gavage

immune system
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, homozygotes failed to develop signs of diarrhea, indicating resistance to enterotoxigenic bacteria that produce heat stable enteroxins
• following an oral inoculum of 10 U of E. coli heat-stable enterotoxin, 3-day suckling homozygotes failed to exhibit any fluid accumulation within the intestinal lumen, as determined by gut/carcass weight ratio
• in contrast, suckling homozygotes showed normal fluid accumulation within the intestine following oral inoculation with 5 ug of cholera toxin
• following inoculation with enterotoxigenic E. coli strain 1676 by gavage, most homozygotes survived to weaning, whereas all wild-type and heterozygous controls died within 72 hrs of infection
• only a small minority of homozygotes died within 24-72 hrs of administration of E. coli strain 1676; however, no diarrhea was observed and no cause of death was established
• following inoculation with a human enterotoxigenic E. coli strain (H10407) which does not colonize and infect mice, 23 of 26 homozygotes survived to weaning, while the remaining 3 died within 24 hrs of the gavage

digestive/alimentary system
• following oral inoculation with 5 ug of cholera toxin, suckling homozygotes show normal fluid accumulation within the intestine (diarrhea), as determined by gut/carcass weight ratio