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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(DO11.10)10Dlo
transgene insertion 10, Dennis Y Loh
MGI:2664398
Summary 28 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Grb2tm1.1Hua/Grb2tm1.1Hua
Tg(DO11.10)10Dlo/0
Tg(Lck-cre)1Jtak/0
involves: 129 * BALB/c * C3H * C57BL/6 MGI:4818271
cn2
Gata3tm1Iho/Gata3tm1Iho
Tg(DO11.10)10Dlo/0
Tg(Lck-cre)1Cwi/0
involves: BALB/c * C3H * C57BL/6 MGI:4417857
cn3
Gata3tm1Iho/Gata3tm1Iho
Tg(Cd4-cre)1Cwi/0
Tg(DO11.10)10Dlo/0
involves: BALB/c * C3H * C57BL/6 * DBA/2 MGI:4417858
cx4
Il2tm1Hor/Il2tm1Hor
Tg(DO11.10)10Dlo/?
C.Cg-Il2tm1Hor Tg(DO11.10)10Dlo MGI:3759780
cx5
Stat2tm1Jdf/Stat2tm1Jdf
Tg(DO11.10)10Dlo/0
C.Cg-Stat2tm1Jdf Tg(DO11.10)10Dlo MGI:3529466
cx6
Slatm1Weis/Slatm1Weis
Tg(DO11.10)10Dlo/0
involves: 129 * BALB/c * C3H * C57BL/6 MGI:3033125
cx7
Tg(DO11.10)10Dlo/0
Crlf2tm1Wjl/Crlf2tm1Wjl
involves: 129 * BALB/c * C3H * C57BL/6 MGI:3624449
cx8
Prkcqtm1Litt/Prkcqtm1Litt
Tg(DO11.10)10Dlo/0
involves: 129 * BALB/c * C3H * C57BL/6 MGI:4843460
cx9
Cd28tm1Pjl/Cd28tm1Pjl
Tg(DO11.10)10Dlo/0
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6 MGI:2664411
cx10
Git2Gt(XG510)Byg/Git2Gt(XG510)Byg
Tg(DO11.10)10Dlo/0
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6 MGI:4461272
cx11
Il13tm1.1Anjm/Il13tm1.1Anjm
Tg(DO11.10)10Dlo/0
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6 MGI:3850558
cx12
Ccr7tm1Rfor/Ccr7tm1Rfor
Tg(DO11.10)10Dlo/?
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6 MGI:4430569
cx13
Nfatc3tm1Glm/Nfatc3tm1Glm
Tg(DO11.10)10Dlo/?
involves: 129S2/SvPas * BALB/c * C3H * C57BL/6 MGI:3525155
cx14
Zap70tm2.1Weis/Zap70tm2.1Weis
Tg(DO11.10)10Dlo/?
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6 MGI:4399134
cx15
Ctla4tm1Shr/Ctla4tm1Shr
Tg(DO11.10)10Dlo/0
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6 MGI:3714354
cx16
Itktm1Litt/Itktm1Litt
Tg(DO11.10)10Dlo/0
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6J MGI:3771808
cx17
Ebi3Gt716Lex/Ebi3Gt716Lex
Tg(DO11.10)10Dlo/0
involves: 129S5/SvEvBrd * BALB/c * C3H * C57BL/6 MGI:3795472
cx18
Igh-Jtm1Mcdl/Igh-Jtm1Mcdl
Igk-Jtm1Mcdl/Igk-Jtm1Mcdl
Rag1tm1Mom/Rag1tm1Mom
Tg(DO11.10)10Dlo/?
involves: 129S/Sv * BALB/c * C3H * C57BL/6 MGI:3586594
cx19
Ctla4tm1Shr/Ctla4tm1Shr
Rag2tm1Fwa/Rag2tm1Fwa
Tg(DO11.10)10Dlo/0
involves: 129S/Sv * BALB/c * C3H * C57BL/6 MGI:3714326
cx20
Tg(DO11.10)10Dlo/?
Toxtm1.1Kay/Toxtm1.1Kay
involves: 129S/Sv * BALB/c * C3H * C57BL/6 MGI:3807780
cx21
Rag2tm1Fwa/Rag2tm1Fwa
Tg(DO11.10)10Dlo/0
involves: 129S/SvEv * BALB/c * C3H * C57BL/6 MGI:3714327
cx22
Btlatm1Kmm/Btlatm1Kmm
Tg(DO11.10)10Dlo/?
involves: 129S/SvEv * BALB/c * C3H * C57BL/6 MGI:3841449
cx23
Hivep2tm1Sis/Hivep2tm1Sis
Tg(DO11.10)10Dlo/?
involves: BALB/cA * C3H * C57BL/6NCrlj * CBA/JNCrlj MGI:3615666
cx24
Sh2d1atm1Pls/Sh2d1atm1Pls
Tg(DO11.10)10Dlo/Tg(DO11.10)10Dlo
involves: BALB/c * C3H * C57BL/6 MGI:3044989
cx25
Ksr1tm1Shaw/Ksr1tm1Shaw
Tg(DO11.10)10Dlo/0
involves: BALB/c * C3H * C57BL/6 MGI:3841456
cx26
Zap70m1Saka/Zap70m1Saka
Tg(DO11.10)10Dlo/0
involves: BALB/c * C3H * C57BL/6 MGI:3698742
cx27
Slamf1tm1Cpt/Slamf1tm1Cpt
Tg(DO11.10)10Dlo/Tg(DO11.10)10Dlo
involves: BALB/c * C3H * C57BL/6 MGI:3044988
tg28
Tg(DO11.10)10Dlo/? involves: BALB/c * C3H * C57BL/6 MGI:3606694


Genotype
MGI:4818271
cn1
Allelic
Composition
Grb2tm1.1Hua/Grb2tm1.1Hua
Tg(DO11.10)10Dlo/0
Tg(Lck-cre)1Jtak/0
Genetic
Background
involves: 129 * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grb2tm1.1Hua mutation (0 available); any Grb2 mutation (41 available)
Tg(DO11.10)10Dlo mutation (7 available)
Tg(Lck-cre)1Jtak mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• positive selection of CD4+ cells is severely impaired compared to in DO11.10 TCR control mice

hematopoietic system
• positive selection of CD4+ cells is severely impaired compared to in DO11.10 TCR control mice




Genotype
MGI:4417857
cn2
Allelic
Composition
Gata3tm1Iho/Gata3tm1Iho
Tg(DO11.10)10Dlo/0
Tg(Lck-cre)1Cwi/0
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata3tm1Iho mutation (0 available); any Gata3 mutation (22 available)
Tg(DO11.10)10Dlo mutation (7 available)
Tg(Lck-cre)1Cwi mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• partial impairment in generation of double positive cells
• partial arrest at the double negative 3 (DN3) to double negative 4 (DN4) transition

immune system
• partial impairment in generation of double positive cells
• partial arrest at the double negative 3 (DN3) to double negative 4 (DN4) transition

endocrine/exocrine glands




Genotype
MGI:4417858
cn3
Allelic
Composition
Gata3tm1Iho/Gata3tm1Iho
Tg(Cd4-cre)1Cwi/0
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: BALB/c * C3H * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata3tm1Iho mutation (0 available); any Gata3 mutation (22 available)
Tg(Cd4-cre)1Cwi mutation (8 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• severe reduction in single positive (SP) CD4 cells and no enrichment of SP CD8 cells

immune system
• severe reduction in single positive (SP) CD4 cells and no enrichment of SP CD8 cells




Genotype
MGI:3759780
cx4
Allelic
Composition
Il2tm1Hor/Il2tm1Hor
Tg(DO11.10)10Dlo/?
Genetic
Background
C.Cg-Il2tm1Hor Tg(DO11.10)10Dlo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2tm1Hor mutation (5 available); any Il2 mutation (32 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• when transferred into athymic mice, CD4 T cells continue to proliferate for up to three weeks in response to antigen
• CD 4 T cells from these transfer experiments also express high levels of activation markers
• However, when transferred with wild-type CD4-postive CD25-positive T cells, the mutant CD4 T cells stop proliferating between one to two weeks after antigen encounter

mortality/aging
• the mean survival time is 4 months, which is significantly shorter than the average life expectancy of BALB/C mic
• however, the mean survival time is much longer than Il2 null mice on a BALB/c background that do not carry the TCR transgene

immune system
• when transferred into athymic mice, CD4 T cells continue to proliferate for up to three weeks in response to antigen
• CD 4 T cells from these transfer experiments also express high levels of activation markers
• However, when transferred with wild-type CD4-postive CD25-positive T cells, the mutant CD4 T cells stop proliferating between one to two weeks after antigen encounter
• when transferred into athymic mice, there are 10-100 fold higher numbers of activated T cells three weeks after antigen
• the abnormal proliferation of CD4 T cells upon transfer into athymic mice is reduced by the addition of wild-type CD4-postive CD25-positive T cells
• this suggests there is a defect in the regulatory T cell population in these mice

hematopoietic system
• when transferred into athymic mice, CD4 T cells continue to proliferate for up to three weeks in response to antigen
• CD 4 T cells from these transfer experiments also express high levels of activation markers
• However, when transferred with wild-type CD4-postive CD25-positive T cells, the mutant CD4 T cells stop proliferating between one to two weeks after antigen encounter
• when transferred into athymic mice, there are 10-100 fold higher numbers of activated T cells three weeks after antigen
• the abnormal proliferation of CD4 T cells upon transfer into athymic mice is reduced by the addition of wild-type CD4-postive CD25-positive T cells
• this suggests there is a defect in the regulatory T cell population in these mice




Genotype
MGI:3529466
cx5
Allelic
Composition
Stat2tm1Jdf/Stat2tm1Jdf
Tg(DO11.10)10Dlo/0
Genetic
Background
C.Cg-Stat2tm1Jdf Tg(DO11.10)10Dlo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat2tm1Jdf mutation (0 available); any Stat2 mutation (22 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• IFN-alpha stimulation does not promote Th1 cell development similar to Stat2 wild-type mice and unlike in humans




Genotype
MGI:3033125
cx6
Allelic
Composition
Slatm1Weis/Slatm1Weis
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129 * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slatm1Weis mutation (1 available); any Sla mutation (17 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• enhanced positive selection, resulting in an increase in the number of cells that mature to the single positive stage relative to wild-type

immune system
• enhanced positive selection, resulting in an increase in the number of cells that mature to the single positive stage relative to wild-type




Genotype
MGI:3624449
cx7
Allelic
Composition
Tg(DO11.10)10Dlo/0
Crlf2tm1Wjl/Crlf2tm1Wjl
Genetic
Background
involves: 129 * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Crlf2tm1Wjl mutation (1 available); any Crlf2 mutation (16 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• when transgenic mutant mice are injected with OVA, there is little induction of CD69+ on CD4+ T cells; CD69+ expression is significantly induced on CD 4+ cells of nontransgenic Tpte2-deficient controls




Genotype
MGI:4843460
cx8
Allelic
Composition
Prkcqtm1Litt/Prkcqtm1Litt
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129 * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcqtm1Litt mutation (3 available); any Prkcq mutation (40 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• induced by Fas

cellular
• induced by Fas

hematopoietic system
• induced by Fas




Genotype
MGI:2664411
cx9
Allelic
Composition
Cd28tm1Pjl/Cd28tm1Pjl
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd28tm1Pjl mutation (0 available); any Cd28 mutation (38 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response
• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes

immune system
• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response
• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes
• thymocyte proliferative responses to OVA antigen are reduced
• 10- to 20-fold decrease in T cell IL-2 production when stimulated with OVA antigen

hematopoietic system
• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response
• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes
• thymocyte proliferative responses to OVA antigen are reduced

endocrine/exocrine glands
• thymocyte proliferative responses to OVA antigen are reduced




Genotype
MGI:4461272
cx10
Allelic
Composition
Git2Gt(XG510)Byg/Git2Gt(XG510)Byg
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Git2Gt(XG510)Byg mutation (3 available); any Git2 mutation (58 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• single positive cell number in thymus
• transgene positive CD4 cells in spleen, lymph node and blood

immune system
• single positive cell number in thymus
• transgene positive CD4 cells in spleen, lymph node and blood




Genotype
MGI:3850558
cx11
Allelic
Composition
Il13tm1.1Anjm/Il13tm1.1Anjm
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il13tm1.1Anjm mutation (0 available); any Il13 mutation (23 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• Th2 T cells from these mice fail to induce airway hyperresponsive when transferred to immunodeficient hosts
• lymphocytes fail to produce IL-13

hematopoietic system
• Th2 T cells from these mice fail to induce airway hyperresponsive when transferred to immunodeficient hosts




Genotype
MGI:4430569
cx12
Allelic
Composition
Ccr7tm1Rfor/Ccr7tm1Rfor
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr7tm1Rfor mutation (1 available); any Ccr7 mutation (18 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• significantly reduced ability of T regulatory cells to enter mesenteric and peripheral lymph nodes
• more moderately reduced ability of T regulatory cells to enter Peyer's patches and the gut
• greater ability to migrate into the liver
• failure of T regulatory cells to localize to T cell zones of lymph nodes
• reduced movement of T regulatory cells away from peripheral sites of inflammation

cellular

hematopoietic system
• significantly reduced ability of T regulatory cells to enter mesenteric and peripheral lymph nodes
• more moderately reduced ability of T regulatory cells to enter Peyer's patches and the gut
• greater ability to migrate into the liver
• failure of T regulatory cells to localize to T cell zones of lymph nodes
• reduced movement of T regulatory cells away from peripheral sites of inflammation




Genotype
MGI:3525155
cx13
Allelic
Composition
Nfatc3tm1Glm/Nfatc3tm1Glm
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: 129S2/SvPas * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nfatc3tm1Glm mutation (1 available); any Nfatc3 mutation (55 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• size reduced 3 fold
• increased apoptosis of double positive cells

immune system
• size reduced 3 fold
• increased apoptosis of double positive cells

endocrine/exocrine glands
• size reduced 3 fold




Genotype
MGI:4399134
cx14
Allelic
Composition
Zap70tm2.1Weis/Zap70tm2.1Weis
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
Zap70tm2.1Weis mutation (1 available); any Zap70 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• a marked reduction in the proportion and absolute numbers of CD4 SP thymocytes, and lower percentages and lower absolute numbers of CD4+ T cells expressing the transgene in the spleens and lymph nodes

immune system
• a marked reduction in the proportion and absolute numbers of CD4 SP thymocytes, and lower percentages and lower absolute numbers of CD4+ T cells expressing the transgene in the spleens and lymph nodes




Genotype
MGI:3714354
cx15
Allelic
Composition
Ctla4tm1Shr/Ctla4tm1Shr
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4tm1Shr mutation (4 available); any Ctla4 mutation (34 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• in recall responses, T cells proliferate more than wild-type Tg(DO11.10)10Dlo cells

growth/size/body
• mild splenomegaly observed in 6-8 week-old mice

hematopoietic system
• mild splenomegaly observed in 6-8 week-old mice
• 3 to 4 days after priming with ovalbumin peptide and antigen-presenting cells, T cells produce Il-4, whereas Ctla4-sufficient, Tg(DO11.10)10Dlo cells do not
• T cells produce Th2 cytokines (Il-4, Il-5, and Il-10) upon restimulation with antigen and APCs, whereas Tg(DO11.10)10Dlo cells produce the Th1 cytokine interferon gamma; Th2 bias is seen over a range of peptide concentration during primary stimulation
• in recall responses, T cells proliferate more than wild-type Tg(DO11.10)10Dlo cells

immune system
• mild splenomegaly observed in 6-8 week-old mice
• 3 to 4 days after priming with ovalbumin peptide and antigen-presenting cells, T cells produce Il-4, whereas Ctla4-sufficient, Tg(DO11.10)10Dlo cells do not
• T cells produce Th2 cytokines (Il-4, Il-5, and Il-10) upon restimulation with antigen and APCs, whereas Tg(DO11.10)10Dlo cells produce the Th1 cytokine interferon gamma; Th2 bias is seen over a range of peptide concentration during primary stimulation
• in recall responses, T cells proliferate more than wild-type Tg(DO11.10)10Dlo cells
• mild lymphadenopathy observed in 6-8 week-old mice




Genotype
MGI:3771808
cx16
Allelic
Composition
Itktm1Litt/Itktm1Litt
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S4/SvJae * BALB/c * C3H * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itktm1Litt mutation (2 available); any Itk mutation (35 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• isolated T cells primed under Th2 conditions for 5 days, restimulated for 5 more days, then restimulated in absence of exogenous cytokine fail to produce Il4, although cell expansion is similar to wild-type; PMA/ionomycin restimulation of Th2-primed cells restores Il4 secretion

hematopoietic system
• isolated T cells primed under Th2 conditions for 5 days, restimulated for 5 more days, then restimulated in absence of exogenous cytokine fail to produce Il4, although cell expansion is similar to wild-type; PMA/ionomycin restimulation of Th2-primed cells restores Il4 secretion




Genotype
MGI:3795472
cx17
Allelic
Composition
Ebi3Gt716Lex/Ebi3Gt716Lex
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S5/SvEvBrd * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ebi3Gt716Lex mutation (0 available); any Ebi3 mutation (9 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• following anti-CD3 antibody stimulation, differentiation into Th17 cells is increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes, Il-22 plasma levels are increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes
• following anti-CD3 antibody stimulation
• mice are resistant to infection with Listeriosis monocytogenes with a 45-fold less bacteria load in the spleen and a 150-fold less bacterial load in the liver after re-challenge compared to in similarly treated wild-type mice

homeostasis/metabolism
• following ovalbumin stimulation or infection with Listeriosis monocytogenes, Il-22 plasma levels are increased compared to in wild-type mice
• following ovalbumin stimulation or infection with Listeriosis monocytogenes

hematopoietic system
• following anti-CD3 antibody stimulation, differentiation into Th17 cells is increased compared to in wild-type mice




Genotype
MGI:3586594
cx18
Allelic
Composition
Igh-Jtm1Mcdl/Igh-Jtm1Mcdl
Igk-Jtm1Mcdl/Igk-Jtm1Mcdl
Rag1tm1Mom/Rag1tm1Mom
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: 129S/Sv * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Mcdl mutation (0 available); any Igh-J mutation (14 available)
Igk-Jtm1Mcdl mutation (0 available); any Igk-J mutation (10 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (89 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the development of IL-4+IFNG- and IFNG+IL-4- T cells after immunization are increased but not if wild-type splenocytes are transferred into mutant mice
• germinal center formation is increased in mutants but not if wild-type splenocytes are transferred into mutant mice
• following immunization with a cross-linked OVA-HA antigen IgE levels are elevated by 2 orders of magnitude compared to mutant mice wild-type for Rag1; however serum Ig half-lives are normal
• following immunization with a cross-linked OVA-HA antigen IgG titers are increased compared to mutant mice wild-type for Rag1, with IgG1 titers increased about 20-fold

hematopoietic system
• the development of IL-4+IFNG- and IFNG+IL-4- T cells after immunization are increased but not if wild-type splenocytes are transferred into mutant mice
• germinal center formation is increased in mutants but not if wild-type splenocytes are transferred into mutant mice
• following immunization with a cross-linked OVA-HA antigen IgE levels are elevated by 2 orders of magnitude compared to mutant mice wild-type for Rag1; however serum Ig half-lives are normal
• following immunization with a cross-linked OVA-HA antigen IgG titers are increased compared to mutant mice wild-type for Rag1, with IgG1 titers increased about 20-fold

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
hyper IgE recurrent infection syndrome 1 DOID:3261 OMIM:147060
J:100072




Genotype
MGI:3714326
cx19
Allelic
Composition
Ctla4tm1Shr/Ctla4tm1Shr
Rag2tm1Fwa/Rag2tm1Fwa
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S/Sv * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctla4tm1Shr mutation (4 available); any Ctla4 mutation (34 available)
Rag2tm1Fwa mutation (46 available); any Rag2 mutation (93 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• mice show no signs of T cell activation, lymphadenopathy, or splenomegaly over 32 weeks; T cells are naive, with no expression of activation markers
• primary response to antigen stimulation in vitro is similar to Rag1-null, Tg(DO11.10)10Dlo T cells
• during secondary response to low-dose antigen stimulation in vitro, T cells show 2.2-3 fold higher proliferation compared to Rag2-null, Tg(Do11.10)10Dlo mice
• transferred T cells are resistant to tolerance induction in vivo in hosts after a tolerizing dose of ovalbumin peptide; in vivo expansion of T cells 4 and 7 days after treatment is 2.2- and 1.4-fold greater than Rag2-null, transgenic mice
• T cells given a tolerogenic stimulus proliferate and secrete Il-2 robustly upon restimulation in contrast to poor response in Rag2-null, Tg(DO11.10)10Dlo mice
• T cells given a tolerogenic stimulus do not exhibit a blockade after entering G1 phase of cell cycle, and readily enter S phase, while Ctla4-sufficient mutant cells do not progress past G1
• during secondary response, on days 2 and 3, T cells show increased production of interferon gamma and Il-4 than transgenic, Rag2-null mice

hematopoietic system
• during secondary response to low-dose antigen stimulation in vitro, T cells show 2.2-3 fold higher proliferation compared to Rag2-null, Tg(Do11.10)10Dlo mice
• transferred T cells are resistant to tolerance induction in vivo in hosts after a tolerizing dose of ovalbumin peptide; in vivo expansion of T cells 4 and 7 days after treatment is 2.2- and 1.4-fold greater than Rag2-null, transgenic mice
• T cells given a tolerogenic stimulus proliferate and secrete Il-2 robustly upon restimulation in contrast to poor response in Rag2-null, Tg(DO11.10)10Dlo mice
• T cells given a tolerogenic stimulus do not exhibit a blockade after entering G1 phase of cell cycle, and readily enter S phase, while Ctla4-sufficient mutant cells do not progress past G1




Genotype
MGI:3807780
cx20
Allelic
Composition
Tg(DO11.10)10Dlo/?
Toxtm1.1Kay/Toxtm1.1Kay
Genetic
Background
involves: 129S/Sv * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
Toxtm1.1Kay mutation (0 available); any Tox mutation (29 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• transgenic T cell development is arrested after positive selection but before CD4+T cell lineage commitment
• mice exhibit severe lymphopenia with only a minor population of CD8+ T cells found in the spleen

hematopoietic system
• transgenic T cell development is arrested after positive selection but before CD4+T cell lineage commitment
• mice exhibit severe lymphopenia with only a minor population of CD8+ T cells found in the spleen




Genotype
MGI:3714327
cx21
Allelic
Composition
Rag2tm1Fwa/Rag2tm1Fwa
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: 129S/SvEv * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1Fwa mutation (46 available); any Rag2 mutation (93 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• T cells exhibit similar levels of activation induced cell death as Rag2-, Ctla4-deficient, Tg (DO11.10)10Dlo T cells
• cells undergo tolerance induction in vivo in response to an immunizing or tolerizing stimulus




Genotype
MGI:3841449
cx22
Allelic
Composition
Btlatm1Kmm/Btlatm1Kmm
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: 129S/SvEv * BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Btlatm1Kmm mutation (3 available); any Btla mutation (21 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• fully polarized TH1 cells show a 2-fold increase in proliferation in response to OVA peptide presented by either CD8+ or CD8-CD11c+ DCs

hematopoietic system
• fully polarized TH1 cells show a 2-fold increase in proliferation in response to OVA peptide presented by either CD8+ or CD8-CD11c+ DCs

immune system
• fully polarized TH1 cells show a 2-fold increase in proliferation in response to OVA peptide presented by either CD8+ or CD8-CD11c+ DCs




Genotype
MGI:3615666
cx23
Allelic
Composition
Hivep2tm1Sis/Hivep2tm1Sis
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: BALB/cA * C3H * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hivep2tm1Sis mutation (2 available); any Hivep2 mutation (80 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• impaired positive selection
• marked reduction in CD4+ single positive t cells after ovalbumin administration relative to controls
• both CD4+ and CD8+ single positive splenocytes reduced after ovalbumin administration
• enhanced generation of TH2 cells
• TH1 differentiation unaffected

hematopoietic system
• impaired positive selection
• marked reduction in CD4+ single positive t cells after ovalbumin administration relative to controls
• both CD4+ and CD8+ single positive splenocytes reduced after ovalbumin administration
• enhanced generation of TH2 cells
• TH1 differentiation unaffected




Genotype
MGI:3044989
cx24
Allelic
Composition
Sh2d1atm1Pls/Sh2d1atm1Pls
Tg(DO11.10)10Dlo/Tg(DO11.10)10Dlo
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation (4 available); any Sh2d1a mutation (10 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• CD4+ T cells produce less IL-4 following treatment with OVA peptide-pulsed antigen presenting cells




Genotype
MGI:3841456
cx25
Allelic
Composition
Ksr1tm1Shaw/Ksr1tm1Shaw
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ksr1tm1Shaw mutation (0 available); any Ksr1 mutation (38 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• decreased T cell proliferation when stimulated with ovalbumin peptide

immune system
• decreased T cell proliferation when stimulated with ovalbumin peptide
• cultured T cells under conditions that promote either Th1 or Th2 differentiation fail to produce appreciable amounts of IFNG or IL4
• addition of supplemental IL2 allows for normal Th1 and Th2 differentiation
• at 24, 48, and 72 h after stimulation compared to controls

hematopoietic system
• decreased T cell proliferation when stimulated with ovalbumin peptide
• cultured T cells under conditions that promote either Th1 or Th2 differentiation fail to produce appreciable amounts of IFNG or IL4
• addition of supplemental IL2 allows for normal Th1 and Th2 differentiation




Genotype
MGI:3698742
cx26
Allelic
Composition
Zap70m1Saka/Zap70m1Saka
Tg(DO11.10)10Dlo/0
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
Zap70m1Saka mutation (1 available); any Zap70 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• T cells require a 10-fold greater concentration of ovalbumin peptide to proliferate to the same extent and wild-type Tg(DO11.10)10Dlo T cells

immune system
• T cells require a 10-fold greater concentration of ovalbumin peptide to proliferate to the same extent and wild-type Tg(DO11.10)10Dlo T cells
• number of CD4+CD8- mature thymocytes decreases to ~20% that in control Tg(DO11.10)10Dlo mice

hematopoietic system
• T cells require a 10-fold greater concentration of ovalbumin peptide to proliferate to the same extent and wild-type Tg(DO11.10)10Dlo T cells
• number of CD4+CD8- mature thymocytes decreases to ~20% that in control Tg(DO11.10)10Dlo mice




Genotype
MGI:3044988
cx27
Allelic
Composition
Slamf1tm1Cpt/Slamf1tm1Cpt
Tg(DO11.10)10Dlo/Tg(DO11.10)10Dlo
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slamf1tm1Cpt mutation (0 available); any Slamf1 mutation (16 available)
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• CD4+ T cells produce less IL-4 following treatment with OVA peptide-pulsed antigen presenting cells




Genotype
MGI:3606694
tg28
Allelic
Composition
Tg(DO11.10)10Dlo/?
Genetic
Background
involves: BALB/c * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(DO11.10)10Dlo mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• 20 hours after administration of OVA323-339 peptide, apoptosis, DNA degradation, chromatin condensation, and cell shrinkage occur in the majority of cortical thymocytes
• apoptosis occurs throughout the thymus including the subcapsular and deep cortical areas
• 3 days after administration of peptide the cortical thymus is acellular; however, normal cellularity exists in the medulla
• peptide administration does not effect small number of thymocytes that express only the beta chain transgene Vb8
• CD4+CD8+TCR cells in thymic cortex are reduced by 55% after administration of OVA 323-339 peptide
• CD4+ CD8- TCR thymocytes are reduced in absolute numbers after administration of OVA 323-339 peptide

hematopoietic system
• 20 hours after administration of OVA323-339 peptide, apoptosis, DNA degradation, chromatin condensation, and cell shrinkage occur in the majority of cortical thymocytes
• apoptosis occurs throughout the thymus including the subcapsular and deep cortical areas
• 3 days after administration of peptide the cortical thymus is acellular; however, normal cellularity exists in the medulla
• peptide administration does not effect small number of thymocytes that express only the beta chain transgene Vb8
• CD4+CD8+TCR cells in thymic cortex are reduced by 55% after administration of OVA 323-339 peptide
• CD4+ CD8- TCR thymocytes are reduced in absolute numbers after administration of OVA 323-339 peptide

endocrine/exocrine glands
• 20 hours after administration of OVA323-339 peptide, apoptosis, DNA degradation, chromatin condensation, and cell shrinkage occur in the majority of cortical thymocytes
• apoptosis occurs throughout the thymus including the subcapsular and deep cortical areas
• 3 days after administration of peptide the cortical thymus is acellular; however, normal cellularity exists in the medulla
• peptide administration does not effect small number of thymocytes that express only the beta chain transgene Vb8





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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory