Phenotypes associated with this allele

• Allele Symbol: Mpl^tm1Mwm
• Allele Name: targeted mutation 1, Mark W Moore
• Allele ID: MGI:2660810
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mpl^tm1Mwm/Mpl^tm1Mwm	B6;129S2	MGI:2662520
cx2	Babam1^Gt(IST11207B9)Tigm/Babam1^Gt(IST11207B9)Tigm Mpl^tm1Mwm/Mpl^tm1Mwm	involves: 129S2/SvPas * C57BL/6N	MGI:6507141

Genotype
MGI:2662520

hm1

• Allelic Composition: Mpl^tm1Mwm/Mpl^tm1Mwm
• Genetic Background: B6;129S2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

absent megakaryocytes ( J:83264 )

• mutant mice displayed a loss of megakaryocytes in the spleen and bone marrow

• however, there was no significant difference in the number of red blood cells, total white blood cells, neutrophils, bands, and esinophils in the peripheral blood

thrombocytopenia ( J:83264 )

• mice displayed an 85% decrease in platelet count

increased mean platelet volume ( J:83264 )

• mutant mice registered 6.25 um³ compared to control animals 4.7 um³

immune system

immune system phenotype ( J:83264 )

N • there was no significant alteration in the size and cellularity of the lymphoid organ

N • no alterations were detected in the ratios of B and T cells examined in the spleen, thymus and bone marrow

Genotype
MGI:6507141

cx2

• Allelic Composition: Babam1^{Gt(IST11207B9)Tigm}/Babam1^{Gt(IST11207B9)Tigm}; Mpl^tm1Mwm/Mpl^tm1Mwm
• Genetic Background: involves: 129S2/SvPas * C57BL/6N

hematopoietic system

thrombocytopenia ( J:223526 )

• double homozygotes show a marked reduction in platelet count, similar to that in single Mpl^tm1Mwm homozygotes

decreased hematopoietic stem cell number ( J:223526 )

• double homozygotes show a marked reduction in the frequency of long-term hematopoietic stem cells (LT-HSCs) (as determined by CD34-Flk2-CD150+ LSK surface markers), similar to that in single Mpl^tm1Mwm homozygotes