Phenotypes associated with this allele

• Allele Symbol: Il9^tm1Anjm
• Allele Name: targeted mutation 1, Andrew NJ McKenzie
• Allele ID: MGI:2656470
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il9^tm1Anjm/Il9^tm1Anjm	involves: 129P2/OlaHsd * C57BL/6	MGI:2656485
hm2	Il9^tm1Anjm/Il9^tm1Anjm	involves: C57BL/6	MGI:3716473
cx3	Il5^tm1Anjm/Il5^tm1Anjm Il9^tm1Anjm/Il9^tm1Anjm	involves: 129P2/OlaHsd * C57BL/6	MGI:3689874
cx4	Il5^tm2Anjm/Il5^tm2Anjm Il9^tm1Anjm/Il9^tm1Anjm	involves: 129P2/OlaHsd * C57BL/6	MGI:3689876
cx5	Il5^tm3Anjm/Il5^tm3Anjm Il9^tm1Anjm/Il9^tm1Anjm	involves: 129P2/OlaHsd * C57BL/6	MGI:3689878

Genotype
MGI:2656485

hm1

• Allelic Composition: Il9^tm1Anjm/Il9^tm1Anjm
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal mast cell morphology ( J:82881 )

• mastocytosis in lungs of infected mice does not occur in mutants; 8-fold fewer mast cells are found compared to wild-type

granulomatous inflammation ( J:82881 , J:113543 )

• granulomas of equivalent size and granularity develop in mutants and wild-type upon infection with parasite eggs (J:82881)

• a secondary challenge induces equivalent levels of goblet cell and mast cell induction, as in wild-type (J:82881)

• upon infection with Schistosoma mansoni eggs, primary granuloma formation and volume is equivalent to wild-type (J:113543)

• volumes of secondary granulomas after second exposure with parasite eggs are similar to wild-type (J:113543)

abnormal response to infection ( J:82881 )

• after primary infection with Schistosoma mansoni eggs, expansion of goblet cell population in airways and mast cell population in lungs does not occur, in contrast to wild-type response

increased susceptibility to parasitic infection ( J:82881 )

• upon nematode infection (N. brasiliensis), fewer goblet cells are found in mutants

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:82881 )

• after primary challenge with parasite eggs, goblet cell hyperplasia along the airway epithelia does not develop like in wild-type; mutants have 10-fold fewer goblet cells than wild-type

• upon nematode infection (N. brasiliensis), fewer goblet cells are found in mutants

hematopoietic system

abnormal mast cell morphology ( J:82881 )

• mastocytosis in lungs of infected mice does not occur in mutants; 8-fold fewer mast cells are found compared to wild-type

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:82881 )

• in jejunal homogenates, mast cell protease (mMCP-1) activity is reduced compared to wild-type after nematode infection

cellular

abnormal intestinal goblet cell morphology ( J:82881 )

• after primary challenge with parasite eggs, goblet cell hyperplasia along the airway epithelia does not develop like in wild-type; mutants have 10-fold fewer goblet cells than wild-type

• upon nematode infection (N. brasiliensis), fewer goblet cells are found in mutants

Genotype
MGI:3716473

hm2

• Allelic Composition: Il9^tm1Anjm/Il9^tm1Anjm
• Genetic Background: involves: C57BL/6

immune system

immune system phenotype ( J:115764 )

N • mice acutely reject skin grafts from Fas^lpr H2Ab-1^bm12 mice similarly to wild-type mice receiving similar grafts, with no modification of graft surivival (median survival time ~14 days)

N • eosinophil recruitment in skin allografts is similar to that observed in wild-type

abnormal cytokine secretion ( J:115764 )

• when stimulated with irradiated H2-Ab1^bm12 allogenic stimulators, Il9r-null spleen cells produced less Il-5 compared to control cells; however, Ifng production in response to allogenic stimulators is comparable between mutant and control cells

Genotype
MGI:3689874

cx3

• Allelic Composition: Il5^tm1Anjm/Il5^tm1Anjm; Il9^tm1Anjm/Il9^tm1Anjm
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased eosinophil cell number ( J:113543 )

• with absence of Il4 and Il5, eosinophilia is completely blocked

abnormal mast cell morphology ( J:113543 )

• mastocytosis is abolished compared to wild-type or triple mutants

abnormal mast cell physiology ( J:113543 )

• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations

abnormal interleukin level ( J:113543 )

• Il-10 expression is reduced compared to Il5^tm1Anjm mutants

abnormal humoral immune response ( J:113543 )

• mice show a switch to a Th1 response and Il12 production from a Th2 response

decreased IgE level ( J:113543 )

• mutants fail to produce IgE, due to deletion of Il4

granulomatous inflammation ( J:113543 )

• primary granuloma formation and volumes in lungs after infection are severely reduced (<10%) compared to wild-type

• secondary granuloma formation is essentially blocked

abnormal susceptibility to infection ( J:113543 )

• expulsion of worms is delayed by an order of magnitude vs triple mutants

increased susceptibility to parasitic infection ( J:113543 )

• compound quadruple mutants show ~1 order of magnitude greater delay in expulsion of worms from intestine (50% remain at day 60 after infection) than triple mutants (35 days)

• more worms remain at 40 days post infection than in triple Il4/Il5/Il13 mutants or wild-type

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:113543 )

• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection

hematopoietic system

decreased eosinophil cell number ( J:113543 )

• with absence of Il4 and Il5, eosinophilia is completely blocked

abnormal mast cell morphology ( J:113543 )

• mastocytosis is abolished compared to wild-type or triple mutants

abnormal mast cell physiology ( J:113543 )

• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations

decreased IgE level ( J:113543 )

• mutants fail to produce IgE, due to deletion of Il4

homeostasis/metabolism

abnormal interleukin level ( J:113543 )

• Il-10 expression is reduced compared to Il5^tm1Anjm mutants

cellular

abnormal intestinal goblet cell morphology ( J:113543 )

• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection

Genotype
MGI:3689876

cx4

• Allelic Composition: Il5^tm2Anjm/Il5^tm2Anjm; Il9^tm1Anjm/Il9^tm1Anjm
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

increased eosinophil cell number ( J:113543 )

• eosinophilia is delayed compared to wild-type, but once induced, correlates with worm clearance

abnormal immunoglobulin level ( J:113543 )

• levels of IgE produced in response to infection are equivalent to wild-type or, after 20 days, keep increasing at 40 days while wild-type levels decrease after 20 days

abnormal interleukin level ( J:113543 )

• IL-4 expression is 4-5 fold greater than wild-type at days 5, 10 and 20 after infection

• IL-10 expression is enhanced compared to wild-type

granulomatous inflammation ( J:113543 )

• primary granuloma formation and volumes in lungs after infection are reduced ~4-fold compared to wild-type

• formation of secondary granuloma formation is reduced compared to wild-type upon reinfection with Schistosoma eggs

abnormal susceptibility to infection ( J:113543 )

• expulsion of worms from intestines is delayed (50% by day 35) compared to wild-type (100% by 10 days)

increased susceptibility to parasitic infection ( J:113543 )

• after N. brasiliensis (nematode) infection, expulsion of worms occurs with similar kinetic to Il4/Il13 double mutants

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:113543 )

• increase in goblet cell number is delayed and occurs after 20 days compared to wild-type which show increased numbers at 10 days

hematopoietic system

increased eosinophil cell number ( J:113543 )

• eosinophilia is delayed compared to wild-type, but once induced, correlates with worm clearance

abnormal immunoglobulin level ( J:113543 )

• levels of IgE produced in response to infection are equivalent to wild-type or, after 20 days, keep increasing at 40 days while wild-type levels decrease after 20 days

homeostasis/metabolism

abnormal interleukin level ( J:113543 )

• IL-4 expression is 4-5 fold greater than wild-type at days 5, 10 and 20 after infection

• IL-10 expression is enhanced compared to wild-type

cellular

abnormal intestinal goblet cell morphology ( J:113543 )

• increase in goblet cell number is delayed and occurs after 20 days compared to wild-type which show increased numbers at 10 days

Genotype
MGI:3689878

cx5

• Allelic Composition: Il5^tm3Anjm/Il5^tm3Anjm; Il9^tm1Anjm/Il9^tm1Anjm
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

granulomatous inflammation ( J:113543 )

• upon infection with Schistosoma mansoni eggs, primary granuloma formation and volume is equivalent to wild-type

• volumes of secondary granulomas after second exposure to parasite eggs are reduced by ~50% compared to wild-type