Analysis Tools
normal phenotype
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• B cell lymphopoiesis in the bone marrow is normal
• the number of B cells found in circulation is comparable to that of wild-type mice
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Allele Symbol Allele Name Allele ID |
Rag2tm1Cgn targeted mutation 1, University of Cologne MGI:2654906 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• B cell lymphopoiesis in the bone marrow is normal
• the number of B cells found in circulation is comparable to that of wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• genomic stability is disrupted in activated B cells with chromosome breakage occurring in close to half of cells
• B cells undergoing class switch recombination have even greater increased risk of chromosome breakage due to aberrations at the Igh locus
• there is decreased breakage at Igl locus compared to conditional knockouts on a Rag-sufficient background
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• class switch recombination often leads to chromosome breakage at the Igh locus
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• class switch recombination often leads to chromosome breakage at the Igh locus
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
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• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
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• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
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• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
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• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
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• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
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• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
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• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
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• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age
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• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
|
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• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
|
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• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
|
|
• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
|
|
• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
|
|
• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
|
|
• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
|
|
• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
|
|
• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• spontaneous EAE develops in two out of six mice
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| multiple sclerosis | DOID:2377 |
OMIM:612594 OMIM:612595 OMIM:612596 |
J:151335 | |
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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