About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Rag2tm1Cgn
targeted mutation 1, University of Cologne
MGI:2654906
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Rag2tm1Cgn/Rag2tm1Cgn C57BL/6-Rag2tm1Cgn MGI:2654914
cn2
Rag2tm1Cgn/Rag2tm1Cgn
Xrcc4tm1Fwa/Xrcc4tm2Fwa
Tg(Cr2-cre)3Cgn/?
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6 MGI:4352688
cn3
Rag2tm1Cgn/Rag2tm1.1Cgn
Tg(Mx1-cre)1Cgn/0
involves: C57BL/6 * CBA MGI:2654913
cx4
Mogtm1Dpd/Mogtm1Dpd
Rag2tm1Cgn/Rag2tm1Cgn
Tg(Tcra2D2,Tcrb2D2)1Kuch/0
involves: 129S2/SvPas * C57BL/6 MGI:4461062


Genotype
MGI:2654914
hm1
Allelic
Composition
Rag2tm1Cgn/Rag2tm1Cgn
Genetic
Background
C57BL/6-Rag2tm1Cgn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1Cgn mutation (2 available); any Rag2 mutation (99 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• B cell lymphopoiesis in the bone marrow is normal
• the number of B cells found in circulation is comparable to that of wild-type mice




Genotype
MGI:4352688
cn2
Allelic
Composition
Rag2tm1Cgn/Rag2tm1Cgn
Xrcc4tm1Fwa/Xrcc4tm2Fwa
Tg(Cr2-cre)3Cgn/?
Genetic
Background
involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1Cgn mutation (2 available); any Rag2 mutation (99 available)
Tg(Cr2-cre)3Cgn mutation (2 available)
Xrcc4tm1Fwa mutation (0 available); any Xrcc4 mutation (23 available)
Xrcc4tm2Fwa mutation (0 available); any Xrcc4 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• genomic stability is disrupted in activated B cells with chromosome breakage occurring in close to half of cells
• B cells undergoing class switch recombination have even greater increased risk of chromosome breakage due to aberrations at the Igh locus
• there is decreased breakage at Igl locus compared to conditional knockouts on a Rag-sufficient background

hematopoietic system
• class switch recombination often leads to chromosome breakage at the Igh locus

immune system
• class switch recombination often leads to chromosome breakage at the Igh locus




Genotype
MGI:2654913
cn3
Allelic
Composition
Rag2tm1Cgn/Rag2tm1.1Cgn
Tg(Mx1-cre)1Cgn/0
Genetic
Background
involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (99 available)
Rag2tm1Cgn mutation (2 available); any Rag2 mutation (99 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age

hematopoietic system
• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age




Genotype
MGI:4461062
cx4
Allelic
Composition
Mogtm1Dpd/Mogtm1Dpd
Rag2tm1Cgn/Rag2tm1Cgn
Tg(Tcra2D2,Tcrb2D2)1Kuch/0
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mogtm1Dpd mutation (0 available); any Mog mutation (55 available)
Rag2tm1Cgn mutation (2 available); any Rag2 mutation (99 available)
Tg(Tcra2D2,Tcrb2D2)1Kuch mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• spontaneous EAE develops in two out of six mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
multiple sclerosis DOID:2377 OMIM:612594
OMIM:612595
OMIM:612596
J:151335





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
09/22/2022
MGI 6.21
The Jackson Laboratory