Phenotypes associated with this allele

• Allele Symbol: Wnt3^tm1Amc
• Allele Name: targeted mutation 1, Andrew P McMahon
• Allele ID: MGI:2450902
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wnt3^tm1Amc/Wnt3^tm1Amc	involves: 129S7/SvEvBrd	MGI:3640401
cn2	Wnt3^tm1Amc/Wnt3^tm2Amc Tg(Msx2-cre)5Rem/0	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ	MGI:2450906
cn3	Wnt3^tm1Amc/Wnt3^tm2Amc Tg(Rarb-cre)1Mrc/0	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ	MGI:2450913

Genotype
MGI:3640401

hm1

• Allelic Composition: Wnt3^tm1Amc/Wnt3^tm1Amc
• Genetic Background: involves: 129S7/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis ( J:81795 )

embryo

failure to gastrulate ( J:81795 )

• homozygotes fail to gastrulate

limbs/digits/tail

absent limbs ( J:81795 )

• homozygotes never develop limbs

Genotype
MGI:2450906

cn2

• Allelic Composition: Wnt3^tm1Amc/Wnt3^tm2Amc; Tg(Msx2-cre)5Rem/0
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ

limbs/digits/tail

abnormal apical ectodermal ridge morphology ( J:81795 )

• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype

thin apical ectodermal ridge ( J:81795 )

• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%

• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected

abnormal autopod morphology ( J:81795 )

• 22 of 44 mutant mice exhibit mild to severe autopod defects

abnormal forelimb morphology ( J:81795 )

• mutant mice display normal forelimbs except in two cases (2/44) where digit 5 was absent or fused to digit 4

abnormal hindlimb morphology ( J:81795 )

• mutant mice display hindlimb defects of variable penetrance and expressivity, ranging from completely normal to entirely absent hindlimbs

absent hindlimb ( J:81795 )

• 3 of 44 mutant mice show complete absence of hindlimbs

short hindlimb ( J:81795 )

• 17 of 44 mutant mice show extensive truncations that extend into more proximal segments of the hindlimb

embryo

increased ectoderm apoptosis ( J:81795 )

• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally

abnormal apical ectodermal ridge morphology ( J:81795 )

• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype

thin apical ectodermal ridge ( J:81795 )

• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%

• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected

cellular

increased ectoderm apoptosis ( J:81795 )

• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally

Genotype
MGI:2450913

cn3

• Allelic Composition: Wnt3^tm1Amc/Wnt3^tm2Amc; Tg(Rarb-cre)1Mrc/0
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ

limbs/digits/tail

abnormal apical ectodermal ridge morphology ( J:81795 )

• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER

abnormal forelimb morphology ( J:81795 )

• mutant mice display variable forelimb defects ranging from three digits (anterior digits 1-3) and normal zeugopod (radius and ulna) to one digit (digit 2 or 3) and also zeugopod defects (missing ulna); in most cases, the humerus appears unaffected

embryo

abnormal apical ectodermal ridge morphology ( J:81795 )

• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER