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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Wnt3tm1Amc
targeted mutation 1, Andrew P McMahon
MGI:2450902
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Wnt3tm1Amc/Wnt3tm1Amc involves: 129S7/SvEvBrd MGI:3640401
cn2
Wnt3tm1Amc/Wnt3tm2Amc
Tg(Msx2-cre)5Rem/0
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ MGI:2450906
cn3
Wnt3tm1Amc/Wnt3tm2Amc
Tg(Rarb-cre)1Mrc/0
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ MGI:2450913


Genotype
MGI:3640401
hm1
Allelic
Composition
Wnt3tm1Amc/Wnt3tm1Amc
Genetic
Background
involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wnt3tm1Amc mutation (0 available); any Wnt3 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

embryo
• homozygotes fail to gastrulate

limbs/digits/tail
• homozygotes never develop limbs




Genotype
MGI:2450906
cn2
Allelic
Composition
Wnt3tm1Amc/Wnt3tm2Amc
Tg(Msx2-cre)5Rem/0
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Msx2-cre)5Rem mutation (2 available)
Wnt3tm1Amc mutation (0 available); any Wnt3 mutation (19 available)
Wnt3tm2Amc mutation (0 available); any Wnt3 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
limbs/digits/tail
• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected
• 22 of 44 mutant mice exhibit mild to severe autopod defects
• mutant mice display normal forelimbs except in two cases (2/44) where digit 5 was absent or fused to digit 4
• mutant mice display hindlimb defects of variable penetrance and expressivity, ranging from completely normal to entirely absent hindlimbs
• 17 of 44 mutant mice show extensive truncations that extend into more proximal segments of the hindlimb
• 3 of 44 mutant mice show complete absence of hindlimbs

embryo
• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally
• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected

cellular
• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally




Genotype
MGI:2450913
cn3
Allelic
Composition
Wnt3tm1Amc/Wnt3tm2Amc
Tg(Rarb-cre)1Mrc/0
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Rarb-cre)1Mrc mutation (0 available)
Wnt3tm1Amc mutation (0 available); any Wnt3 mutation (19 available)
Wnt3tm2Amc mutation (0 available); any Wnt3 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
limbs/digits/tail
• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER
• mutant mice display variable forelimb defects ranging from three digits (anterior digits 1-3) and normal zeugopod (radius and ulna) to one digit (digit 2 or 3) and also zeugopod defects (missing ulna); in most cases, the humerus appears unaffected

embryo
• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER





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last database update
10/16/2018
MGI 6.12
The Jackson Laboratory