• mice exhibit normal survival following diethylnitrosamine (DEN) challenged (J:173666)

• double negative to double positive differentiation is blocked unlike in wild-type mice

• double negative to double positive differentiation is blocked unlike in wild-type mice

• double negative to double positive differentiation is blocked unlike in wild-type mice

• pro-T cells exhibit excessive cell death unlike wild-type cells

• early lymphoid progenitors and early thymic precursors fail to differentiate into mature T cells in the presence of Il7 unlike in wild-type mice

• however, inhibition of SOCS1 or overexpression of Bcl2 restores T cell differentiation in vitro

• alphabeta-T cells are reduced 1000-fold compared to in wild-type mice

• early T-cell precursors in the thymus are reduced compared to in wild-type mice

• DN1a-e subsets are decreased 70- to 130-fold compared to in wild-type mice

• DN1a/b cells are decreased 230-fold compared to in wild-type mice

• 100-fold reduction in DN2a cells

• 40-fold reduction in DN2b cells

• 3-fold reduction in DN3a cells

• 10-fold reduction in DN3b cells

• 7-fold

• 100-fold reduction in cellularity

• early lymphoid progenitors and early thymic precursors fail to differentiate into mature T cells in the presence of Il7 unlike in wild-type mice

• however, inhibition of SOCS1 or overexpression of Bcl2 restores T cell differentiation in vitro

• alphabeta-T cells are reduced 1000-fold compared to in wild-type mice

• early T-cell precursors in the thymus are reduced compared to in wild-type mice

• DN1a-e subsets are decreased 70- to 130-fold compared to in wild-type mice

• DN1a/b cells are decreased 230-fold compared to in wild-type mice

• 100-fold reduction in DN2a cells

• 40-fold reduction in DN2b cells

• 3-fold reduction in DN3a cells

• 10-fold reduction in DN3b cells

• 7-fold

• 100-fold reduction in cellularity

• pro-T cells exhibit excessive cell death unlike wild-type cells

• early T-cell precursors in the thymus are reduced compared to in wild-type mice

• DN1a-e subsets are decreased 70- to 130-fold compared to in wild-type mice

• DN1a/b cells are decreased 230-fold compared to in wild-type mice

• 100-fold reduction in DN2a cells

• 40-fold reduction in DN2b cells

• 3-fold reduction in DN3a cells

• 10-fold reduction in DN3b cells

• early lymphoid progenitors and early thymic precursors fail to differentiate into mature T cells in the presence of Il7 unlike in wild-type mice

• however, inhibition of SOCS1 or overexpression of Bcl2 restores T cell differentiation in vitro

• V(D)J recombination in B and T cells is normal (J:139510)

• in a competitive reconstitution assay, cells fail to repopulate the B and T cell population as well as do wild type cells

• fewer cells undergo the switch from IgM to IgG1 or IgG3 compared to in wild type mice

• however, there is no increase in Igh chromosomal aberrations

• in the thymus

• the number of mature and re-circulating B cells is decreased compared to in wild-type mice

• however, the numbers of pro- and pre-B cells is normal

• in a competitive reconstitution assay, cells fail to repopulate the B and T cell population as well as do wild type cells

• fewer cells undergo the switch from IgM to IgG1 or IgG3 compared to in wild type mice

• however, there is no increase in Igh chromosomal aberrations

• in the thymus

• the number of mature and re-circulating B cells is decreased compared to in wild-type mice

• however, the numbers of pro- and pre-B cells is normal

• in a competitive reconstitution assay, cells fail to repopulate the B and T cell population as well as do wild type cells

• fewer cells undergo the switch from IgM to IgG1 or IgG3 compared to in wild type mice

• however, there is no increase in Igh chromosomal aberrations

• in the thymus

• mice exhibit an increase in ProE and Ery.A fractions but a decrease in Ery.B and Ery.C in the bone marrow compared with control mice

• mice exhibit an increase in ProE and Ery.A fractions but a decrease in Ery.C in the spleen compared with control mice

• early erythropoiesis is partially blocked in association with a cell cycle defect

• proerythroblast exhibit reduced proliferation compared with control cells

• however, proerythroblast apoptosis rates are normal

• intrinsic as determined by bone marrow transplantation

• ProE cells are increased 3.6- and 74-fold in the bone marrow and spleen compared with control mice

• in the bone marrow

• 4.7-fold in the spleen

• secondary

• decrease in the granulocyte macrophage progenitor (GMP) cells in the bone marrow

• increase in the megakaryocyte-erythroid progenitor (MEP) cells in the spleen

• decrease in the granulocyte macrophage progenitor (GMP) cells in the bone marrow

• early long and short term hematopoietic stem cells are increased compared to in control mice

• however, the number of lymphoid-primed multipotent progenitors is normal and the increased in hematopoietic stem cells disappears in the alter stages of differentiation

• in adult mice

• without affecting architecture

• reduced half-life

• in adult mice

• proerythroblast exhibit reduced proliferation compared with control cells

• at birth

• in adult mice

• without affecting architecture

• c-Kit+B220+ pro-B cells and c-Kit-B220+ developmental stages are rescued (J:137719)

• mice lack committed c-Kit+CD19+ pro-B cells

• mice lack committed c-Kit+CD19+ pro-B cells

• mice lack committed c-Kit+CD19+ pro-B cells

• development of pro-B cells is rescued (J:137719)

• proximal VH7183-DJH and distal VHJ558-DJH rearrangements of the IgH are reduced compared to in wild-type mice

• Vk-Jk recombination of the Igk locus is absent

• proximal VH7183-DJH and distal VHJ558-DJH rearrangements of the IgH are reduced compared to in wild-type mice

• Vk-Jk recombination of the Igk locus is absent

• proximal VH7183-DJH and distal VHJ558-DJH rearrangements of the IgH are reduced compared to in wild-type mice

• Vk-Jk recombination of the Igk locus is absent

• transplanted bone marrow progenitors transfected with BCR-ABL and NUP98-HOXA9 proliferate slower than similarly treated wild-type cells improving host survival

• mice exhibit normal hematopoietical parameters in peripheral blood and normal bone marrow cell numbers (J:186700)

• increase in common lymphocyte progenitor cells in the bone marrow and spleen

• increase in megakaryocyte-erythroid progenitors in the spleen

• increase in LSK and lineage-restricted progenitors in the bone marrow and spleen

• increase in LK in the spleen

• increase in megakaryocyte-erythroid progenitors in the spleen

• NK cells fail to make IFN-gamma in response to injections of TLR9 agonists

• NK cells in vivo have a blunted IFN-gamma response to LPS injections

• unlike null homozygotes, mice survive to adulthood (J:181715)

• at E14.5, mice exhibit blood-filled vessels unlike wild-type mice

• there is about a 3-fold reduction in pre-B cell CFUs isolated from bone marrow

• there is about a 2-fold reduction in myeloid CFU number isolated from E13.5 fetal livers

• a similar reduction in CFU is observed with bone marrow cells

• mutant HSC bone marrow cells give less then a 1% long-term reconstitution when transplanted at a 1:1 ratio with wild-type bone marrow into irradiated mice

• mutant HSC bone marrow cells give only a 2% long-term reconstitution when transplanted at a 10:1 ratio

• poor reconstitution is observable as little as 4 weeks after transfer

• there is about a 3-fold reduction in pre-B cell CFUs isolated from bone marrow

• there is about a 3-fold reduction in pre-B cell CFUs isolated from bone marrow

• mice exhibit normal expression of Cd8a and Cd8b (J:179326)

• exhibit normal blood cell counts, bone marrow composition, bone marrow and spleen cellularity, and reconstitution capacity (J:180431)

• 2-fold as in Kitl^tm2.2Sjm heterozygotes in the bone marrow

• unlike null homozygotes, mice survive to adulthood (J:181715)

• at E14.5, mice exhibit blood-filled vessels unlike control mice

• at 8 weeks, dye injection confirms interconnection between lymphatic and blood vessels with rapid spread labeling unlike in control mice

• at 8 weeks, dye injection confirms interconnection between lymphatic and blood vessels with rapid spread labeling unlike in control mice

• at E14.5

• similar to the phenotype in mice carrying Tg(CMV-cre)1Cgn and Tg(Kit*D814V)1Roer

• similar to the phenotype in mice carrying Tg(CMV-cre)1Cgn and Tg(Kit*D814V)3Roer

• mice exhibit normal hematopoietical parameters in peripheral blood and normal bone marrow cell numbers (J:186700)

• increase in common lymphocyte progenitor cells in the bone marrow and spleen

• increase in megakaryocyte-erythroid progenitors in the spleen

• increase in LSK and lineage-restricted progenitors in the bone marrow and spleen

• increase in LK in the spleen

• increase in megakaryocyte-erythroid progenitors in the spleen

• mice exhibit normal T cell development and autoimmunity (J:177784)

• in the bone marrow

• immature and re-circulating B cells

• in the spleen

• in the spleen

• in the spleen

• increased numbers in the spleen

• 1.5-fold but not as severe as in Rc3h1^san homozygotes

• 1.5-fold

• 1.5-fold

• some are disrupted

• 1.5-fold

• mice exhibit increased spontaneous germinal center formation compared with control mice

• in the bone marrow

• in the bone marrow

• immature and re-circulating B cells

• in the spleen

• in the spleen

• in the spleen

• increased numbers in the spleen

• 1.5-fold but not as severe as in Rc3h1^san homozygotes

• 1.5-fold

• 1.5-fold

• some are disrupted

• 1.5-fold

• reduction in most myeloid progenitors

• numbers of myeloid subsets CD11b⁺Gr1^- and CD11b^-Gr1⁺ are reduced in blood and decrease further over time

• numbers of CD11b⁺Gr1⁺ cells are increased in the blood at 5 and 6 weeks of age but then decrease to below wild-type levels at 8 weeks of age

• CD11b⁺Gr1⁺ cells in the spleen and bone marrow are increased and show higher proliferation rates

• mutants exhibit presence of atypical myeloid infiltrates in a wide range of organs

• mutants are cytopenic for all blood leukocyte populations except for CD11b⁺Gr1⁺ cells

• absolute cell counts of B cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• immature NK cells (Lin^-CD3^-CD122⁺NK1.1⁺DX5⁺) are depleted in the bone marrow

• absolute cell counts of NK cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• absolute cell counts of T cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• common lymphoid progenitors (Lin^-Flt3^HighIL7Ra^HighSca-1^Lowc-Kit^Low) are reduced in mutants

• numbers of CCR9⁺ lymphoid-primed multipotent progenitors are reduced in the bone marrow of mutants

• overall bone marrow cell count is reduced

• mutant LSK (Lin^-Sca-1⁺c-Kit⁺) cells accumulate mitochondria, mitochondrial superoxide, and DNA damage and exhibit increased levels of apoptosis and proliferation

• numbers of hematopoietic stem cells are reduced in all mutants, regardless of disease progression

• absolute numbers of LSK (Lin^-Sca-1⁺c-Kit⁺) cells significantly increased in asymptomatic 7 week old mutants, however as they develop symptoms, the frequency of LSK cells falls to wild-type levels

• the Lin^-Sca-1^-c-Kit⁺ (LK) compartment, containing more mature hematopoietic progenitors, is decreased

• adult bone marrow cells from mutants transplanted together with CD45.1⁺ wild-type bone marrow cells into lethally irradiated wild-type hosts fail to contribute to reconstitution of cells in the hosts while in noncompetitive reconstitution experiments, lethally irradiated mice die within 4 weeks after transplantation with mutant bone marrow cells, indicating loss of hematopoietic stem cell activity

• numbers of CD11b⁺Gr1⁺ cells are increased in the blood at 5 and 6 weeks of age but then decrease to below wild-type levels at 8 weeks of age

• CD11b⁺Gr1⁺ cells in the spleen and bone marrow are increased and show higher proliferation rates

• mutants exhibit presence of atypical myeloid infiltrates in a wide range of organs

• mutants are cytopenic for all blood leukocyte populations except for CD11b⁺Gr1⁺ cells

• absolute cell counts of B cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• immature NK cells (Lin^-CD3^-CD122⁺NK1.1⁺DX5⁺) are depleted in the bone marrow

• absolute cell counts of NK cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• absolute cell counts of T cells are decreased in the peripheral blood of mutants and numbers drop steadily over time

• common lymphoid progenitors (Lin^-Flt3^HighIL7Ra^HighSca-1^Lowc-Kit^Low) are reduced in mutants

• numbers of CCR9⁺ lymphoid-primed multipotent progenitors are reduced in the bone marrow of mutants