Phenotypes associated with this allele

• Allele Symbol: Anxa1^tm1Rjf
• Allele Name: targeted mutation 1, Roderick J Flower
• Allele ID: MGI:2449710
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Anxa1^tm1Rjf/Anxa1^tm1Rjf	involves: 129 * C57BL	MGI:5431553
hm2	Anxa1^tm1Rjf/Anxa1^tm1Rjf	involves: 129 * C57BL/6	MGI:5431551
hm3	Anxa1^tm1Rjf/Anxa1^tm1Rjf	involves: BALB/c	MGI:5431550
hm4	Anxa1^tm1Rjf/Anxa1^tm1Rjf	involves: C57BL/6	MGI:5431554
hm5	Anxa1^tm1Rjf/Anxa1^tm1Rjf	Not Specified	MGI:2653815
ht6	Anxa1^tm1Rjf/Anxa1^+	Not Specified	MGI:3046060
cx7	Anxa1^tm1Rjf/Anxa1^tm1Rjf Tg(MMTV-PyVT)634Mul/?	involves: FVB/N	MGI:5431552

Genotype
MGI:5431553

hm1

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf
• Genetic Background: involves: 129 * C57BL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:129587 , J:135972 )

N • basal corticosterone output by the adrenal is normal (J:129587)

N • basal circulation ACTH levels are normal (J:135972)

increased adrenocorticotropin level ( J:135972 )

• ACTH in the anterior pituitary is significantly increased in males

endocrine/exocrine glands

thin adrenal cortex ( J:129587 )

• decreased adreno-cortical thickness

abnormal corticotroph morphology ( J:135972 )

• granule area significantly reduced but proportional to cell size

• corticotrophs in males have a clustered distribution

decreased corticotroph cell size ( J:135972 )

abnormal folliculo-stellate cell morphology ( J:135972 )

• cytoplasmic area of folliculo-stellate cells is increased in both males and females

• nuclear area of folliculo-stellate cells is increased in males only

• IL-6 in folliculo-stellate cells is increased in males but decreased in females

abnormal gonadotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

abnormal lactotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

abnormal somatotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

abnormal adrenal gland secretion ( J:129587 )

• increased corticosterone response to ACTH

• basal aldosterone output and ACTH stimulated output are normal

nervous system

abnormal corticotroph morphology ( J:135972 )

• granule area significantly reduced but proportional to cell size

• corticotrophs in males have a clustered distribution

decreased corticotroph cell size ( J:135972 )

abnormal folliculo-stellate cell morphology ( J:135972 )

• cytoplasmic area of folliculo-stellate cells is increased in both males and females

• nuclear area of folliculo-stellate cells is increased in males only

• IL-6 in folliculo-stellate cells is increased in males but decreased in females

abnormal gonadotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

abnormal lactotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

abnormal somatotroph morphology ( J:135972 )

• rough endoplasmic reticulum is significantly increased

Genotype
MGI:5431551

hm2

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf
• Genetic Background: involves: 129 * C57BL/6

neoplasm

abnormal tumor vascularization ( J:153690 )

• blood vessel density in both B6 melanoma and Lewis Lung Carcinoma 1/3 that in tumors from control mice

decreased metastatic potential ( J:153690 )

• no Lewis Lung Carcinoma metastasis found at death in tumor bearing mice

decreased tumor growth/size ( J:153690 )

• both B6 melanoma and Lewis Lung Carcinoma cell growth greatly retarded, 1/4 the size of control mouse tumors after 7 weeks

• control mouse Lewis Lung Carcinoma tumors are 5X the size of mutant mouse tumors at 10 weeks

• increased levels of necrosis in developing tumors of both B6 melanoma and Lewis Lung Carcinoma

cellular

abnormal vascular endothelial cell migration ( J:153690 )

• 70% less migration of aortic endothelial cells

decreased vascular endothelial cell proliferation ( J:153690 )

• 70% less proliferation of aortic endothelial cells

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:153690 )

• better survival of mice implanted with either B6 melanoma or, particularly, Lewis Lung Carcinoma

homeostasis/metabolism

delayed wound healing ( J:153690 )

• sutured incisions take up to 32 days to heal compared to 16 days for comparable wounds in controls

cardiovascular system

abnormal tumor vascularization ( J:153690 )

• blood vessel density in both B6 melanoma and Lewis Lung Carcinoma 1/3 that in tumors from control mice

abnormal vascular endothelial cell migration ( J:153690 )

• 70% less migration of aortic endothelial cells

abnormal vascular endothelial cell physiology ( J:153690 )

• considerably reduced vascular endothelium cell sprouting in aortic ring cultures

decreased vascular endothelial cell proliferation ( J:153690 )

• 70% less proliferation of aortic endothelial cells

Genotype
MGI:5431550

hm3

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf
• Genetic Background: involves: BALB/c

growth/size/body

weight loss ( J:142735 )

• more pronounced weight loss than in controls within 3 days of treatment with dextran sodium sulfate to induce colitis

• no improvement in body weight in the 7 days following treatment

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:142735 )

• epithelial loss continues after the end of dextran sodium sulfate treatment

abnormal colon morphology ( J:142735 )

• colon weight to weight ratio is increased about 18% after 7 days of dextran sodium sulfate treatment

increased susceptibility to induced colitis ( J:142735 )

• severity of disease remains elevated during the course of dextran sodium sulfate treatment compared to controls

• more rectal bleeding than in controls

• more histological injury than in controls

• no disease improvement until 7 days after the end of dextran sodium sulfate treatment

mortality/aging

increased susceptibility to xenobiotic induced morbidity/mortality ( J:142735 )

• 25% mortality when dextran sodium sulfate treatment is ended compared to zero mortality in controls

homeostasis/metabolism

increased susceptibility to xenobiotic induced morbidity/mortality ( J:142735 )

• 25% mortality when dextran sodium sulfate treatment is ended compared to zero mortality in controls

immune system

increased susceptibility to induced colitis ( J:142735 )

• severity of disease remains elevated during the course of dextran sodium sulfate treatment compared to controls

• more rectal bleeding than in controls

• more histological injury than in controls

• no disease improvement until 7 days after the end of dextran sodium sulfate treatment

Genotype
MGI:5431554

hm4

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf
• Genetic Background: involves: C57BL/6

skeleton

abnormal cranium morphology ( J:129618 )

abnormal sagittal suture morphology ( J:129618 )

• delayed development in 1 day old mice

• prominent gap between frontal bones in 1 day old mice

• persists in 5 day old mice

abnormal neurocranium morphology ( J:129618 )

abnormal frontal bone morphology ( J:129618 )

• modest delay in intramembranous ossification near the nasal septum and between the orbits

• frontal bones of adults are thicker and more mineralized

abnormal palatine bone morphology ( J:129618 )

• palatine bone in 5 day old mice is much closer to the epithelial layer of the oral cavity

• "non-radial" growth pattern found in 5 day old mice

ectopic cranial bone ( J:129618 )

• interfrontal bone appears in 5 day old mice and persists into adults

increased bone mineral density ( J:129618 )

• increased about 25% in males over control males

craniofacial

abnormal cranium morphology ( J:129618 )

abnormal sagittal suture morphology ( J:129618 )

• delayed development in 1 day old mice

• prominent gap between frontal bones in 1 day old mice

• persists in 5 day old mice

abnormal neurocranium morphology ( J:129618 )

abnormal frontal bone morphology ( J:129618 )

• modest delay in intramembranous ossification near the nasal septum and between the orbits

• frontal bones of adults are thicker and more mineralized

abnormal palatine bone morphology ( J:129618 )

• palatine bone in 5 day old mice is much closer to the epithelial layer of the oral cavity

• "non-radial" growth pattern found in 5 day old mice

ectopic cranial bone ( J:129618 )

• interfrontal bone appears in 5 day old mice and persists into adults

Genotype
MGI:2653815

hm5

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf
• Genetic Background: Not Specified

Anxa1^tm1Rjf/Anxa1^tm1Rjf mice exhibit a delay in colonic mucosal wound closure

immune system

abnormal leukocyte physiology ( J:81498 )

• polymorphonuclear leucocytes from homozygotes show increased spontaneous migratory behavior in vivo

• in vitro, leukocytes from mutant mice display altered adhesion molecule expression

impaired macrophage phagocytosis ( J:81498 )

• mutant peritoneal lavage macrophages exhibit a defect in phagocytosis

increased inflammatory response ( J:81498 )

• homozygotes display an enhanced sensitivity to carrageenin-induced inflammation (paw edema)

• dexamethasone fails to inhibit the first phase of carrageenin-induced paw edema; in contrast, there is no change in glucocorticoid sensitivity of the second phase

• in zymosan-induced peritonitis, leukocyte emigration and IL-1beta generation is enhanced in homozygotes of both sexes compared to wild-type

• after 4 hours zymosan peritonitis, a significant change is observed in the disposition of peripheral blood cells in female, though not in male, homozygotes; this is primarily accounted for by a severe decrease in circulating polymorphonuclear cells

adipose tissue

abnormal adipose tissue morphology ( J:115806 )

• elevated IL-6 levels in the epididymal fat pad

• resting cAMP levels in adipose tissue are significantly lower

abnormal fat pad morphology ( J:115806 )

• epididymal fat pad weight is lower

• adipocyte size is normal

abnormal adipose tissue physiology ( J:115806 )

• attenuated glycerol release and cAMP release in response to isoprenalin

• attenuated lipolytic response to both isoprenalin and norepinephrin, increase in glycerol release does not increase as much as in controls

• basal IL-6 release by tissue explants is significantly reduced and is unresponsive to catacholamine treatment

homeostasis/metabolism

increased circulating insulin level ( J:115806 )

delayed wound healing ( J:194294 )

• at day 4 in a colonic mucosal wound assay

hematopoietic system

abnormal leukocyte physiology ( J:81498 )

• polymorphonuclear leucocytes from homozygotes show increased spontaneous migratory behavior in vivo

• in vitro, leukocytes from mutant mice display altered adhesion molecule expression

impaired macrophage phagocytosis ( J:81498 )

• mutant peritoneal lavage macrophages exhibit a defect in phagocytosis

cellular

impaired macrophage phagocytosis ( J:81498 )

• mutant peritoneal lavage macrophages exhibit a defect in phagocytosis

Genotype
MGI:3046060

ht6

• Allelic Composition: Anxa1^tm1Rjf/Anxa1⁺
• Genetic Background: Not Specified

immune system

increased inflammatory response ( J:81498 )

• in zymosan-induced peritonitis, leukocyte emigration is enhanced in heterozygotes of both sexes compared to wild-type

Genotype
MGI:5431552

cx7

• Allelic Composition: Anxa1^tm1Rjf/Anxa1^tm1Rjf; Tg(MMTV-PyVT)634Mul/?
• Genetic Background: involves: FVB/N

neoplasm

decreased metastatic potential ( J:174635 )

• metastasis to lungs is reduced

decreased tumor growth/size ( J:174635 )

• although tumor susceptibility is normal, tumor growth is reduced