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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nkx2-5tm2(cre)Rph
targeted mutation 2, Richard P Harvey
MGI:2448972
Summary 12 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
ht1
Nkx2-5tm2(cre)Rph/Nkx2-5tm4Rph involves: 129S1/Sv MGI:5426984
cn2
Nkx2-5tm2(cre)Rph/Nkx2-5+
Pbx1tm1.1Koss/Pbx1tm1.1Koss
involves: 129P2/OlaHsd * 129S1/Sv MGI:5426979
cn3
Cdkn2btm1Bbd/Cdkn2btm1Bbd
Pbx1tm1Koss/Pbx1tm1Koss
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129P2/OlaHsd * 129S1/Sv MGI:5426986
cn4
Pbx1tm1Koss/Pbx1tm1Koss
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129P2/OlaHsd * 129S1/Sv MGI:5426980
cn5
Pbx1tm1Koss/Pbx1tm1Koss
Pbx2tm1Mlc/Pbx2+
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129P2/OlaHsd * 129S/Sv * 129S1/Sv MGI:5426982
cn6
Hccstm1Tcc/Hccstm1Tcc
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv MGI:3826965
cn7
Hccstm1Tcc/Y
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv MGI:3826966
cn8
Hccstm1Tcc/Hccs+
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv MGI:3826967
cn9
Gas2l3tm1c(EUCOMM)Hmgu/Gas2l3tm1c(EUCOMM)Hmgu
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv * 129S4/SvJaeSor * C57BL/6N MGI:6116294
cn10
Hccstm1Tcc/Hccs+
Nkx2-5tm2(cre)Rph/Nkx2-5+
Tg(CAG-EGFP)D4Nagy/0
Tg(Hmgcr-lacZ)H253Sest/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2 MGI:3826982
cn11
Bmp2tm1Vrs/Bmp2tm1Vrs
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv * C57BL/6 MGI:3641420
cn12
Smyd2tm1.1Fben/Smyd2tm1.1Fben
Nkx2-5tm2(cre)Rph/Nkx2-5+
involves: 129S1/Sv * C57BL/6 MGI:4441343


Genotype
MGI:5426984
ht1
Allelic
Composition
Nkx2-5tm2(cre)Rph/Nkx2-5tm4Rph
Genetic
Background
involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Nkx2-5tm4Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system

immune system




Genotype
MGI:5426979
cn2
Allelic
Composition
Nkx2-5tm2(cre)Rph/Nkx2-5+
Pbx1tm1.1Koss/Pbx1tm1.1Koss
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Pbx1tm1.1Koss mutation (0 available); any Pbx1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• hypoplastic and fragmented

immune system
• hypoplastic and fragmented




Genotype
MGI:5426986
cn3
Allelic
Composition
Cdkn2btm1Bbd/Cdkn2btm1Bbd
Pbx1tm1Koss/Pbx1tm1Koss
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2btm1Bbd mutation (1 available); any Cdkn2b mutation (2 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Pbx1tm1Koss mutation (0 available); any Pbx1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• partial rescue of spleen expansion compared to mutant mice wild-type for Cdkn2b and spleens form a single compact anlage
• significant rescue of mesenchymal proliferation compared to mutant mice wild-type for Cdkn2b

immune system
• partial rescue of spleen expansion compared to mutant mice wild-type for Cdkn2b and spleens form a single compact anlage
• significant rescue of mesenchymal proliferation compared to mutant mice wild-type for Cdkn2b




Genotype
MGI:5426980
cn4
Allelic
Composition
Pbx1tm1Koss/Pbx1tm1Koss
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Pbx1tm1Koss mutation (0 available); any Pbx1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• hypoplastic and fragmented
• significant decrease of mitotic mesenchymal cells in the anlagen

immune system
• hypoplastic and fragmented
• significant decrease of mitotic mesenchymal cells in the anlagen




Genotype
MGI:5426982
cn5
Allelic
Composition
Pbx1tm1Koss/Pbx1tm1Koss
Pbx2tm1Mlc/Pbx2+
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129P2/OlaHsd * 129S/Sv * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Pbx1tm1Koss mutation (0 available); any Pbx1 mutation (18 available)
Pbx2tm1Mlc mutation (0 available); any Pbx2 mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• hypoplastic and fragmented
• more severe than in mutant mice wild-type for Pbx2

immune system
• hypoplastic and fragmented
• more severe than in mutant mice wild-type for Pbx2




Genotype
MGI:3826965
cn6
Allelic
Composition
Hccstm1Tcc/Hccstm1Tcc
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hccstm1Tcc mutation (2 available); any Hccs mutation (4 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most die between E10.5 and E12.5 with few surviving to die by E14.5




Genotype
MGI:3826966
cn7
Allelic
Composition
Hccstm1Tcc/Y
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hccstm1Tcc mutation (2 available); any Hccs mutation (4 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most die between E10.5 and E12.5 with few surviving to die by E14.5

cardiovascular system
• at E13.5, the degree of cardiomyocyte differentiation towards the ventricular lumen is lower than in wild-type mice
• at E13.5, cardiomyocytes have less mature sarcomeres with shorter, randomly arranged muscle fibrils unlike in wild-type mice
• at E13.5, a subset of cardiomyocytes contain irregular mitochondria
• however, mice exhibit normal cardiac morphology at E10.5 and E12.5
• at E13.5, cardiomyocytes accumulate a fine granular material unlike in wild-type cells
• at E11.5, proliferation of cardiomyocytes is decreased 45% compared to in wild-type mice

cellular
• at E11.5, proliferation of cardiomyocytes is decreased 45% compared to in wild-type mice
• at E12.5 and E14.5, respiratory chain complex III activity in cardiomyocytes is 10% of normal

homeostasis/metabolism
• at E13.5, glycogen storage in embryonic hearts is decreased compared to in wild-type mice

muscle
• at E11.5, proliferation of cardiomyocytes is decreased 45% compared to in wild-type mice




Genotype
MGI:3826967
cn8
Allelic
Composition
Hccstm1Tcc/Hccs+
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hccstm1Tcc mutation (2 available); any Hccs mutation (4 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 13% of mice die between 9 and 15 months of age with varying degrees of dilated cardiomyopathy
• however, mice exhibit no embryonic lethality

cardiovascular system
• at 8 weeks, pale and granulated cells resembling degenerating cardiomyocytes are found in the ventricular myocardium unlike in wild-type mice
• at 1 year, severely affected mice exhibit hypertrophic cardiocyocytes
• at E13.5, a subset of cardiomyocytes contain irregular mitochondria
• however, no other cardiac abnormalities are observed at E13.5
• in severely affected mice at 1 year
• in severely affected mice at 1 year
• in severely affected mice at 1 year
• in severely affected mice at 1 year
• in mice with hypertrophic cardiomyocytes at 1 year
• at 1 year, mice with dilated cardiomyopathy exhibit increased interstitial fibrosis compared to mice that develop a hypertrophic cardiomyocyte-like phenotype
• in severely affected mice at 1 year
• at 1 year, 40% of mice exhibit pathologies of the cardiac conduction system including first degree atrioventricular (AV) block or intermittent second degree AV block type II
• at 1 year, 40% of mice exhibit pathologies of the cardiac conduction system including sinus bradycardia
• at 1 year, 40% of mice exhibit pathologies of the cardiac conduction system including transient bundle branch block
• respiratory chain complex III activity in cardiomyocytes is reduced 43% at E12.5 and 56% at E14.5 compared to in wild-type cells
• however, cardiomyocytes is reduced at 8 weeks is normal

cellular
• respiratory chain complex III activity in cardiomyocytes is reduced 43% at E12.5 and 56% at E14.5 compared to in wild-type cells
• however, cardiomyocytes is reduced at 8 weeks is normal

homeostasis/metabolism
• in severely affected mice at 1 year

muscle
• at 8 weeks, pale and granulated cells resembling degenerating cardiomyocytes are found in the ventricular myocardium unlike in wild-type mice
• at 1 year, severely affected mice exhibit hypertrophic cardiocyocytes
• in severely affected mice at 1 year




Genotype
MGI:6116294
cn9
Allelic
Composition
Gas2l3tm1c(EUCOMM)Hmgu/Gas2l3tm1c(EUCOMM)Hmgu
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * 129S4/SvJaeSor * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gas2l3tm1c(EUCOMM)Hmgu mutation (0 available); any Gas2l3 mutation (20 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• increase in heart to body weight ratio

muscle




Genotype
MGI:3826982
cn10
Allelic
Composition
Hccstm1Tcc/Hccs+
Nkx2-5tm2(cre)Rph/Nkx2-5+
Tg(CAG-EGFP)D4Nagy/0
Tg(Hmgcr-lacZ)H253Sest/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hccstm1Tcc mutation (2 available); any Hccs mutation (4 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Tg(CAG-EGFP)D4Nagy mutation (2 available)
Tg(Hmgcr-lacZ)H253Sest mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at E12.5, the relative volume of abnormal heart tissue begins to decline to 42% in ventricles and 47% in atria and is further reduced at E16.5 to 18% in ventricles and 19% in the atria then 10% in ventricles and 17% in the atria prior to birth
• at E12.5 and E13.5, cardiomyocytes appear small and round compared to wild-type cells
• however, the absolute volume of heart tissue before birth is unchanged
• while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner

muscle
• while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner

cellular
• while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner




Genotype
MGI:3641420
cn11
Allelic
Composition
Bmp2tm1Vrs/Bmp2tm1Vrs
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bmp2tm1Vrs mutation (0 available); any Bmp2 mutation (4 available)
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• conditional Bmp2 deletion results in death by E11.5; embryos are alive at E10.5

embryo
• conditional Bmp2-null embryos are smaller than wild-type controls at E10.5

growth/size/body
• conditional Bmp2-null embryos are smaller than wild-type controls at E10.5

cardiovascular system
• at E10.5, embryos have an abnormally patterned heart with an uncharacteristic, straight morphology to the region between the atria and ventricles
• endocardial cushions and EC mesenchyme are missing at E10.5, as well as the AV constriction
• some embryos show pericardial effusion

homeostasis/metabolism
• some embryos show pericardial effusion
• some embryos show cardiac edema




Genotype
MGI:4441343
cn12
Allelic
Composition
Smyd2tm1.1Fben/Smyd2tm1.1Fben
Nkx2-5tm2(cre)Rph/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx2-5tm2(cre)Rph mutation (1 available); any Nkx2-5 mutation (14 available)
Smyd2tm1.1Fben mutation (0 available); any Smyd2 mutation (132 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• no defects are detected in cardiac morphology or function





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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory