Analysis Tools
immune system
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• homozygotes display a significant 7-fold decrease in the basal eosinophil content of the small intestine, along with a 6-fold increase in the spleen
• in contrast, the basal eosinophil content of lung tissue and thymus remains normal relative to wild-type mice
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• homozygotes show a 7-fold reduction in the basal eosinophil content of the small intestine relative to wild-type mice
(J:74509)
• in a model of allergic skin inflammation elicited by epicutaneous allergen application, eosinophils are virtually absent in the skin of homozygotes following epicutaneous OVA sensitization
(J:75347)
• no eosinophil product MBP staining is detectable in either sham-sensitized or epicutaneously OVA-sensitized skin sites of homozygous mutant mice
(J:75347)
• eosinophils are absent in bronchoalveolar lavage fluid after Aspergillus fumigatus exposure
(J:115273)
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• naive homozygotes show a 6-fold increase in the basal eosinophil content of the spleen relative to naive wild-type mice, with eosinophils found in and around the red pulp areas, esp. under the capsule and out in the peripheral sinusoids of the spleen
• at 6 and 24 hrs after the last aerosolized ovalbumin (OA) challenge, homozygotes show an increase in splenic eosinophil numbers relative to wild-type mice, suggesting that eosinophils not recruited to sensitized and challenged lung home alternatively to the spleen
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• reduced monocyte recruitment into bronchoalveolar lavage fluid after Aspergillus fumigatus exposure
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• homozygotes display impaired basal trafficking of eosinophils to the mucosal tissues of the gastrointestinal tract but not the lung
(J:74509)
• in a model of allergic skin inflammation elicited by epicutaneous allergen application, homozygotes exhibit no increase in eosinophils or eosinophil product major basic protein (MBP) in the skin after epicutaneous sensitization with OVA
(J:75347)
• homozygotes epicutaneously sensitized with OVA do not develop BAL or lung tissue eosinophilia following inhalation challenge with allergen
(J:75347)
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• OA-challenged homozygotes display a selective reduction of eosinophil migration into the airways, with eosinophils abnormally trapped within the blood vessels and located under the endothelial cells instead of migrating out into the lung parenchyma
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• at 6 and 24 hrs after the last aerosolized OA challenge, sensitized challenged homozygotes show a 50%-70% decrease in eosinophil trafficking to both the airway lumen and lung tissue relative to wild-type mice
(J:74509)
• homozygotes epicutaneously sensitized with OVA display impaired recruitment of eosinophils to the lung parenchyma and BAL fluid
(J:75347)
|
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• OA-challenged homozygotes show a 4- to 12-fold increase in the number of mast cells found in the submucosal and epithelial layers of large bronchi relative to wild-type control mice, indicating increased mast cell trafficking within the airways after allergen challenge
• however, normal numbers of mast cells are found in the small intestine, skin, and spleen, indicating that this increase is specific to the airway
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• in some sections, intraepithelial mast cells found in OA-challenged homozygotes appear to be partially degranulated
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• after Aspergillus fumigatus exposure
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respiratory system
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• reduced mucus production after Aspergillus fumigatus exposure
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• homozygotes epicutaneously sensitized with OVA fail to develop increased airway responsiveness to methacholine following antigen inhalation
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• despite a marked reduction in eosinophil trafficking to the lung, OA-challenged homozygotes display a significantly greater airway hyperresponsiveness to inhaled methacholine relative to OA-challenged wild-type mice
• however, allergen challenge induces a normal Th2 response with respect to IL-4 and IL-13 levels, with no differences in serum levels of specific OA-IgE relative to wild-type mice
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hematopoietic system
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• homozygotes show normal T and B cell phenotypes and normal hematologic parameters, including leukocyte differential counts, platelets, and hematocrit
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• homozygotes display a significant 7-fold decrease in the basal eosinophil content of the small intestine, along with a 6-fold increase in the spleen
• in contrast, the basal eosinophil content of lung tissue and thymus remains normal relative to wild-type mice
|
|
• homozygotes show a 7-fold reduction in the basal eosinophil content of the small intestine relative to wild-type mice
(J:74509)
• in a model of allergic skin inflammation elicited by epicutaneous allergen application, eosinophils are virtually absent in the skin of homozygotes following epicutaneous OVA sensitization
(J:75347)
• no eosinophil product MBP staining is detectable in either sham-sensitized or epicutaneously OVA-sensitized skin sites of homozygous mutant mice
(J:75347)
• eosinophils are absent in bronchoalveolar lavage fluid after Aspergillus fumigatus exposure
(J:115273)
|
|
• naive homozygotes show a 6-fold increase in the basal eosinophil content of the spleen relative to naive wild-type mice, with eosinophils found in and around the red pulp areas, esp. under the capsule and out in the peripheral sinusoids of the spleen
• at 6 and 24 hrs after the last aerosolized ovalbumin (OA) challenge, homozygotes show an increase in splenic eosinophil numbers relative to wild-type mice, suggesting that eosinophils not recruited to sensitized and challenged lung home alternatively to the spleen
|
|
• reduced monocyte recruitment into bronchoalveolar lavage fluid after Aspergillus fumigatus exposure
|
|
• homozygotes display impaired basal trafficking of eosinophils to the mucosal tissues of the gastrointestinal tract but not the lung
(J:74509)
• in a model of allergic skin inflammation elicited by epicutaneous allergen application, homozygotes exhibit no increase in eosinophils or eosinophil product major basic protein (MBP) in the skin after epicutaneous sensitization with OVA
(J:75347)
• homozygotes epicutaneously sensitized with OVA do not develop BAL or lung tissue eosinophilia following inhalation challenge with allergen
(J:75347)
|
|
• OA-challenged homozygotes display a selective reduction of eosinophil migration into the airways, with eosinophils abnormally trapped within the blood vessels and located under the endothelial cells instead of migrating out into the lung parenchyma
|
|
• at 6 and 24 hrs after the last aerosolized OA challenge, sensitized challenged homozygotes show a 50%-70% decrease in eosinophil trafficking to both the airway lumen and lung tissue relative to wild-type mice
(J:74509)
• homozygotes epicutaneously sensitized with OVA display impaired recruitment of eosinophils to the lung parenchyma and BAL fluid
(J:75347)
|
|
• OA-challenged homozygotes show a 4- to 12-fold increase in the number of mast cells found in the submucosal and epithelial layers of large bronchi relative to wild-type control mice, indicating increased mast cell trafficking within the airways after allergen challenge
• however, normal numbers of mast cells are found in the small intestine, skin, and spleen, indicating that this increase is specific to the airway
|
|
• in some sections, intraepithelial mast cells found in OA-challenged homozygotes appear to be partially degranulated
|
homeostasis/metabolism
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• after Aspergillus fumigatus exposure
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• more modest increase in coagulation components after allergen stimulation than seen in controls
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cellular
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• OA-challenged homozygotes display a selective reduction of eosinophil migration into the airways, with eosinophils abnormally trapped within the blood vessels and located under the endothelial cells instead of migrating out into the lung parenchyma
|
|
• in some sections, intraepithelial mast cells found in OA-challenged homozygotes appear to be partially degranulated
|
