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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Snai2tm2Grid
targeted mutation 2, Tom Gridley
MGI:2447176
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Snai2tm2Grid/Snai2tm2Grid involves: 129S1/Sv MGI:2652604
hm2
Snai2tm2Grid/Snai2tm2Grid involves: 129S1/Sv * C57BL/6 MGI:3715217
cn3
Meox2tm1(cre)Sor/Meox2+
Snai1tm1Grid/Snai1tm2Grid
Snai2tm2Grid/Snai2tm2Grid
involves: 129S1/Sv * 129S4/SvJaeSor * C57BL/6 MGI:3715232
cn4
Snai1tm1Grid/Snai1tm2Grid
Snai2tm2Grid/Snai2tm2Grid
H2az2Tg(Wnt1-cre)11Rth/0
involves: 129S1/Sv * C57BL/6 * CBA MGI:3715216
cx5
Snai2tm2Grid/Snai2tm2Grid
Snai3tm1.2Jhws/Snai3tm1.2Jhws
involves: 129/Sv * 129S1/Sv * C57BL/6 MGI:5620202
cx6
Snai2tm2Grid/Snai2+
Snai3tm1.2Jhws/Snai3tm1.2Jhws
involves: 129/Sv * 129S1/Sv * C57BL/6 MGI:5620264
cx7
Snai2tm2Grid/Snai2tm2Grid
Snai3tm1.2Jhws/Snai3+
involves: 129/Sv * 129S1/Sv * C57BL/6 MGI:5620265
cx8
KitW-v/Kit+
Snai2tm2Grid/Snai2tm2Grid
involves: 129S1/Sv * C57BL MGI:2652596
cx9
KitW-v/Kit+
Snai2tm2Grid/Snai2+
involves: 129S1/Sv * C57BL MGI:2652598
cx10
Snai1tm1Grid/Snai1+
Snai2tm2Grid/Snai2tm2Grid
involves: 129S1/Sv * C57BL/6 MGI:3715218


Genotype
MGI:2652604
hm1
Allelic
Composition
Snai2tm2Grid/Snai2tm2Grid
Genetic
Background
involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
pigmentation
• white forehead blaze occasionally noted
• occasional white spotting of coat (J:78323)
• mutants exhibit areas of hypopigmentation on the midforehead and extremity depigmentation (J:80529)
• various degrees of dermal depigmentation; tails and feet are often depigmented
• areas of hypopigmentation on the midforehead

craniofacial
• areas of hypopigmentation on the midforehead

behavior/neurological
• genetic background effects were noted, but not specified (J:78323)
• genetic background effects were noted, but not specified (J:78323)

endocrine/exocrine glands
• apoptotic cells were noted in the cortical level
• despite the reduced size of the tubules, spermatogenesis appeared normal
• testes were reduced by 40% compared to controls

growth/size/body
• areas of hypopigmentation on the midforehead
• at time of weaning animals weighed 70% that of wild-type and heterozygous littermates
• animals showed moderate growth retardation during the first 3 weeks of life
• after weaning, growth rate matches that of wild-type but animals never attain wild-type body size

hematopoietic system
• apoptotic cells were noted in the cortical level
• a partial block in T cell differentiation in the thymus resulted in fewer mature CD4+/CD8+ cells

immune system
• apoptotic cells were noted in the cortical level
• a partial block in T cell differentiation in the thymus resulted in fewer mature CD4+/CD8+ cells
• variable degrees of inflammation; almost all homozygotes develop swollen eyelids
• variable degrees of neutrophilic infiltration

reproductive system
• despite the reduced size of the tubules, spermatogenesis appeared normal
• testes were reduced by 40% compared to controls
• small litters from homozygous males were produced; on average the litter size was 3-6 pups vs. 10-12 in controls
• incomplete penetrance; 15% of males fail to induce pregnancy despite producing plugs in females
• small litters from homozygous males were produced; on average the litter size was 3-6 pups vs. 10-12 in controls

vision/eye
• variable degrees of inflammation; almost all homozygotes develop swollen eyelids
• variable degrees of neutrophilic infiltration
• histological analysis revealed a number of conjunctival abnormalities

integument
• white forehead blaze occasionally noted
• occasional white spotting of coat (J:78323)
• mutants exhibit areas of hypopigmentation on the midforehead and extremity depigmentation (J:80529)
• various degrees of dermal depigmentation; tails and feet are often depigmented
• areas of hypopigmentation on the midforehead

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Waardenburg syndrome type 2D DOID:0110952 OMIM:608890
J:80529




Genotype
MGI:3715217
hm2
Allelic
Composition
Snai2tm2Grid/Snai2tm2Grid
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes with cleft palate die shortly after birth

craniofacial
• ~50% of homozygotes display cleft secondary palate
• at E15.0 palatal shelves elevate normally and come together at midline, but fail to form medial epithelial seam and fuse

digestive/alimentary system
• ~50% of homozygotes display cleft secondary palate
• at E15.0 palatal shelves elevate normally and come together at midline, but fail to form medial epithelial seam and fuse

growth/size/body
• ~50% of homozygotes display cleft secondary palate
• at E15.0 palatal shelves elevate normally and come together at midline, but fail to form medial epithelial seam and fuse




Genotype
MGI:3715232
cn3
Allelic
Composition
Meox2tm1(cre)Sor/Meox2+
Snai1tm1Grid/Snai1tm2Grid
Snai2tm2Grid/Snai2tm2Grid
Genetic
Background
involves: 129S1/Sv * 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Meox2tm1(cre)Sor mutation (3 available); any Meox2 mutation (15 available)
Snai1tm1Grid mutation (0 available); any Snai1 mutation (7 available)
Snai1tm2Grid mutation (1 available); any Snai1 mutation (7 available)
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• double mutants display an open neural tube at E9.5

growth/size/body

nervous system
N
• in E8.5 cranial fold explants cultured for 48 hours, neural crest cell delamination and migration are observed, similar to control explants
• double mutants display an open neural tube at E9.5




Genotype
MGI:3715216
cn4
Allelic
Composition
Snai1tm1Grid/Snai1tm2Grid
Snai2tm2Grid/Snai2tm2Grid
H2az2Tg(Wnt1-cre)11Rth/0
Genetic
Background
involves: 129S1/Sv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2az2Tg(Wnt1-cre)11Rth mutation (2 available); any H2az2 mutation (25 available)
Snai1tm1Grid mutation (0 available); any Snai1 mutation (7 available)
Snai1tm2Grid mutation (1 available); any Snai1 mutation (7 available)
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• resulting from cleft palate

craniofacial
• enlarged frontal foramen in neonates
• nenates have shortened parietal bones
• mandible is shorter than in wild-type
• in neonates
• the rostral portion of the Meckels cartilage is missing in neonates
• anterior palatal shelves of some double null mutants show subtle size and shape differences relative to wild-type palates at E13.5 and 14.5
• neonates show cleft palate
• palatal shelves remain in a vertical growth orientation and fail to elevate

skeleton
• enlarged frontal foramen in neonates
• nenates have shortened parietal bones
• mandible is shorter than in wild-type
• in neonates
• the rostral portion of the Meckels cartilage is missing in neonates

digestive/alimentary system
• anterior palatal shelves of some double null mutants show subtle size and shape differences relative to wild-type palates at E13.5 and 14.5
• neonates show cleft palate
• palatal shelves remain in a vertical growth orientation and fail to elevate

growth/size/body
• anterior palatal shelves of some double null mutants show subtle size and shape differences relative to wild-type palates at E13.5 and 14.5
• neonates show cleft palate
• palatal shelves remain in a vertical growth orientation and fail to elevate




Genotype
MGI:5620202
cx5
Allelic
Composition
Snai2tm2Grid/Snai2tm2Grid
Snai3tm1.2Jhws/Snai3tm1.2Jhws
Genetic
Background
involves: 129/Sv * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
Snai3tm1.2Jhws mutation (0 available); any Snai3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• approximately 50% fewer homozygotes are produced than expected from heterozygote x heterozygote matings
• fewer homozygote females are produced than expected from heterozygote x heterozygote matings
• few mice survive beyond 15 weeks of age

growth/size/body
• mice are approximately half the size and weight of controls

hematopoietic system
• altered organization of thymic cortex
• altered organization of thymic medulla
• thymus is smaller than wild-type controls
• thymus is fibrotic
• thymus is mostly involuted
• reduced numbers of thymic CD4+ CD8+ cells
• increased neutrophil (Cd11b+ Gr1Hi) population in bone marrow, spleen and blood
• decrease in numbers of B220+ CD19+ cells in bone marrow
• reduced numbers of B220+ CD19+ cells in the blood; phenotype is more severe in comparison to that observed in Snai3+/-Snai2-/- and Snai3-/-Snai2+/- mice
• reduced numbers of B220+ CD19+ cells in the spleen
• increase in CD4+ single positive cells
• increased macrophage (Cd11b+ Gr1Int) population in bone marrow and spleen
• spleen is approximately one third the size of heterozygote controls at 4 weeks of age
• extensive follicle disorganization is observed in spleens from 6 week old animals
• phenotype is more severe in comparison to Snai3+/-Snai2-/- and Snai3-/-Snai2+/-
• spleen follicles are ringed with a lighter, cell deficient zone

immune system
• altered organization of thymic cortex
• altered organization of thymic medulla
• thymus is smaller than wild-type controls
• thymus is fibrotic
• thymus is mostly involuted
• reduced numbers of thymic CD4+ CD8+ cells
• increased neutrophil (Cd11b+ Gr1Hi) population in bone marrow, spleen and blood
• decrease in numbers of B220+ CD19+ cells in bone marrow
• reduced numbers of B220+ CD19+ cells in the blood; phenotype is more severe in comparison to that observed in Snai3+/-Snai2-/- and Snai3-/-Snai2+/- mice
• reduced numbers of B220+ CD19+ cells in the spleen
• increase in CD4+ single positive cells
• increased macrophage (Cd11b+ Gr1Int) population in bone marrow and spleen
• spleen is approximately one third the size of heterozygote controls at 4 weeks of age
• extensive follicle disorganization is observed in spleens from 6 week old animals
• phenotype is more severe in comparison to Snai3+/-Snai2-/- and Snai3-/-Snai2+/-
• spleen follicles are ringed with a lighter, cell deficient zone

endocrine/exocrine glands
• altered organization of thymic cortex
• altered organization of thymic medulla
• thymus is smaller than wild-type controls
• thymus is fibrotic
• thymus is mostly involuted

reproductive system
• males do not produce offspring

vision/eye
• eye abnormalities are observed




Genotype
MGI:5620264
cx6
Allelic
Composition
Snai2tm2Grid/Snai2+
Snai3tm1.2Jhws/Snai3tm1.2Jhws
Genetic
Background
involves: 129/Sv * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
Snai3tm1.2Jhws mutation (0 available); any Snai3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• altered thymic cortex organization
• altered thymic medulla organization

hematopoietic system
• altered thymic cortex organization
• altered thymic medulla organization
• reduced numbers of B220+ CD19+ cells in the blood
• phenotype is less severe than that observed in Snai3-/-Snai2-/- and Snai3-/+Snai2-/- mice
• follicle disorganization observed in spleens from 6 week old animals
• phenotype is less severe than that observed in Snai3-/-Snai2-/- mice

immune system
• altered thymic cortex organization
• altered thymic medulla organization
• reduced numbers of B220+ CD19+ cells in the blood
• phenotype is less severe than that observed in Snai3-/-Snai2-/- and Snai3-/+Snai2-/- mice
• follicle disorganization observed in spleens from 6 week old animals
• phenotype is less severe than that observed in Snai3-/-Snai2-/- mice




Genotype
MGI:5620265
cx7
Allelic
Composition
Snai2tm2Grid/Snai2tm2Grid
Snai3tm1.2Jhws/Snai3+
Genetic
Background
involves: 129/Sv * 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
Snai3tm1.2Jhws mutation (0 available); any Snai3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• altered thymic cortex organization
• altered thymic medulla organization

hematopoietic system
• altered thymic cortex organization
• altered thymic medulla organization
• increase in number of neutrophils (Cd11b+ Gr1Hi) in the blood from 6 month old mice
• reduced numbers of B220+ CD19+ cells in the blood
• phenotype is intermediate between Snai3-/-Snai2-/- and Snai3-/-Snai2+/-
• increase in number of macrophages (Cd11b+ Gr1Int) in the blood from 6 month old mice
• follicle disorganization observed in spleens from 6 week old animals, however, phenotype is less severe than that observed in Snai3-/-Snai2-/- mice

immune system
• altered thymic cortex organization
• altered thymic medulla organization
• increase in number of neutrophils (Cd11b+ Gr1Hi) in the blood from 6 month old mice
• reduced numbers of B220+ CD19+ cells in the blood
• phenotype is intermediate between Snai3-/-Snai2-/- and Snai3-/-Snai2+/-
• increase in number of macrophages (Cd11b+ Gr1Int) in the blood from 6 month old mice
• follicle disorganization observed in spleens from 6 week old animals, however, phenotype is less severe than that observed in Snai3-/-Snai2-/- mice




Genotype
MGI:2652596
cx8
Allelic
Composition
KitW-v/Kit+
Snai2tm2Grid/Snai2tm2Grid
Genetic
Background
involves: 129S1/Sv * C57BL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
KitW-v mutation (11 available); any Kit mutation (149 available)
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
pigmentation
• extensive white spotting of coat was noted from birth

integument
• extensive white spotting of coat was noted from birth




Genotype
MGI:2652598
cx9
Allelic
Composition
KitW-v/Kit+
Snai2tm2Grid/Snai2+
Genetic
Background
involves: 129S1/Sv * C57BL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
KitW-v mutation (11 available); any Kit mutation (149 available)
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
pigmentation
• extensive white spotting of coat was noted from birth

integument
• extensive white spotting of coat was noted from birth




Genotype
MGI:3715218
cx10
Allelic
Composition
Snai1tm1Grid/Snai1+
Snai2tm2Grid/Snai2tm2Grid
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Snai1tm1Grid mutation (0 available); any Snai1 mutation (7 available)
Snai2tm2Grid mutation (1 available); any Snai2 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

craniofacial
• penetrance is 100%
• dramatic reduction in apoptotic cells is observed at intersection of palatal shelves at E14.5

digestive/alimentary system
• penetrance is 100%
• dramatic reduction in apoptotic cells is observed at intersection of palatal shelves at E14.5

growth/size/body
• penetrance is 100%
• dramatic reduction in apoptotic cells is observed at intersection of palatal shelves at E14.5





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory