Phenotypes associated with this allele

• Allele Symbol: Tg(Tagln-tTA)1Mrab
• Allele Name: transgene insertion 1, Marlene Rabinovitch
• Allele ID: MGI:2447082
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Tagln-tTA)1Mrab/0 Tg(tetO-Mcpt1)1Mrab/0	involves: C57BL/6 * SJL	MGI:3772652
tg2	Tg(Tagln-tTA)1Mrab/0	involves: C57BL/6 * SJL	MGI:3772653

Genotype
MGI:3772652

cx1

• Allelic Composition: Tg(Tagln-tTA)1Mrab/0; Tg(tetO-Mcpt1)1Mrab/0
• Genetic Background: involves: C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal artery morphology ( J:70031 )

• medial to lumen ratio of mesenteric artery is increased by 93% compared to single transgenic controls; thickening is reversed by doxycycline-induced suppression of transgene expression

abnormal aorta tunica media morphology ( J:70031 )

• increase in media to lumen ratio of aorta is observed in binary transgenic mice

increased heart left ventricle weight ( J:70031 )

• modest increase in left ventricle weight to body weight is observed in transgene expressing mice

increased mean systemic arterial blood pressure ( J:70031 )

• MAP is increased to 103 mm Hg compared to 82 mm Hg in controls

increased systemic arterial systolic blood pressure ( J:70031 )

• increased by an average of 27 mm Hg relative to littermate controls; 24% increase in blood pressure in double mutants is seen compared to single transgenic mice

• doxycycline treatment to suppress transgene expression completely reverses increase, returning blood pressure to control values

abnormal vasoconstriction ( J:70031 )

• endothelium-dependent (methacholine-induced) relaxation in pre-constricted arteries is impaired relative to controls

increased vasoconstriction ( J:70031 )

• isolated mesenteric arteries display significantly enhanced constriction in response to phenylephrine treatment relative to control arteries

cellular

increased cell proliferation ( J:70031 )

• abundant expression of proliferating cells is observed of medial layer of mesenteric artery in double mutants

muscle

abnormal vasoconstriction ( J:70031 )

• endothelium-dependent (methacholine-induced) relaxation in pre-constricted arteries is impaired relative to controls

increased vasoconstriction ( J:70031 )

• isolated mesenteric arteries display significantly enhanced constriction in response to phenylephrine treatment relative to control arteries

Genotype
MGI:3772653

tg2

• Allelic Composition: Tg(Tagln-tTA)1Mrab/0
• Genetic Background: involves: C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected ( J:70031 )

• mice show no apparent abnormalities; blood pressure is normal