Phenotypes associated with this allele

• Allele Symbol: Pik3cd^tm1Jni
• Allele Name: targeted mutation 1, James N Ihle
• Allele ID: MGI:2446574
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pik3cd^tm1Jni/Pik3cd^tm1Jni	involves: 129P2/OlaHsd	MGI:3796414
hm2	Pik3cd^tm1Jni/Pik3cd^tm1Jni	involves: 129P2/OlaHsd * C57BL/6	MGI:3796420
cx3	Pik3cd^tm1Jni/Pik3cd^tm1Jni Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd * C57BL/6	MGI:3796421

Genotype
MGI:3796414

hm1

• Allelic Composition: Pik3cd^tm1Jni/Pik3cd^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell number ( J:80614 )

• the total number of bone marrow B-lineage cells is decreased 25% compared to in wild-type mice

decreased mature B cell number ( J:80614 )

• mice have fewer B220+CD43-, B220+IgM+ and B220++IgM^low mature B cells compared to in wild-type mice

• mice exhibit reduced total B220+ splenocytes compared to in wild-type mice

absent B-1 B cells ( J:80614 )

• mice lack CD5+ B1 B cells in the peritoneal lymphocytes

small spleen ( J:80614 )

absent spleen germinal center ( J:80614 )

abnormal B cell physiology ( J:80614 )

• stimulated B cells only product an increase in intracellular calcium that is 25% of that observed in wild-type cells

decreased B cell proliferation ( J:80614 )

decreased immunoglobulin level ( J:80614 )

• mice exhibit lower levels of all immunoglobulins, with IgM and IgG1 levels the most decreased, compared to in wild-type mice

small lymph nodes ( J:80614 )

abnormal humoral immune response ( J:80614 )

• IgM and IgG1 response to TNP-LPS treatment is 30% to 50% lower than in wild-type mice

• IgM, IgG1, IgG2b and IgG3 response to TNP-Ficoll is lower than in wild-type mice

• mice immunized with TNP-KLH fail to mount any response unlike wild-type mice

hematopoietic system

decreased B cell number ( J:80614 )

• the total number of bone marrow B-lineage cells is decreased 25% compared to in wild-type mice

decreased mature B cell number ( J:80614 )

• mice have fewer B220+CD43-, B220+IgM+ and B220++IgM^low mature B cells compared to in wild-type mice

• mice exhibit reduced total B220+ splenocytes compared to in wild-type mice

absent B-1 B cells ( J:80614 )

• mice lack CD5+ B1 B cells in the peritoneal lymphocytes

small spleen ( J:80614 )

absent spleen germinal center ( J:80614 )

abnormal B cell physiology ( J:80614 )

• stimulated B cells only product an increase in intracellular calcium that is 25% of that observed in wild-type cells

decreased B cell proliferation ( J:80614 )

decreased immunoglobulin level ( J:80614 )

• mice exhibit lower levels of all immunoglobulins, with IgM and IgG1 levels the most decreased, compared to in wild-type mice

cellular

decreased B cell proliferation ( J:80614 )

Genotype
MGI:3796420

hm2

• Allelic Composition: Pik3cd^tm1Jni/Pik3cd^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

immune system phenotype ( J:124330 )

N • splenic NK cells are phenotypically indistinguishable from wild-type

Genotype
MGI:3796421

cx3

• Allelic Composition: Pik3cd^tm1Jni/Pik3cd^tm1Jni; Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal NK cell differentiation ( J:124330 )

• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice

• NK cell development is blocked before the stage at which they acquire Ly49 receptors

decreased NK cell number ( J:124330 )

• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice

decreased T cell number ( J:124330 )

• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice

spleen hypoplasia ( J:124330 )

• total splenocyte numbers are reduced 50% compared to in wild-type mice

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are less lytic than wild-type cells

decreased interferon-gamma secretion ( J:124330 )

• mice exhibit decreased IFN-gamma secretion following NK1.1 or IL-12/IL-18 stimulation compared to in wild-type mice

hematopoietic system

abnormal NK cell differentiation ( J:124330 )

• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice

• NK cell development is blocked before the stage at which they acquire Ly49 receptors

decreased NK cell number ( J:124330 )

• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice

decreased T cell number ( J:124330 )

• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice

spleen hypoplasia ( J:124330 )

• total splenocyte numbers are reduced 50% compared to in wild-type mice

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are less lytic than wild-type cells