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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cdh1tm2Kem
targeted mutation 2, Rolf Kemler
MGI:2389020
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
ht1
Cdh1tm2Kem/Cdh1tm5(Cdh2)Kem involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3697488
cn2
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Vil1-cre)20Syr/0
involves: 129 * C57BL/6 * DBA/2 MGI:5634401
cn3
Cdh1tm1.1Mpst/Cdh1tm2Kem
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Tg(Vil1-cre)20Syr/0
involves: 129 * C57BL/6 * DBA/2 MGI:4822000
cn4
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(MMTV-cre)7Mul/0
involves: 129 * C57BL/6 * FVB/N MGI:5634403
cn5
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
involves: 129 * C57BL/6 * FVB/N MGI:5634400
cn6
Cdh1tm2Kem/Cdh1tm2Kem
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
involves: 129 * C57BL/6 * FVB/N MGI:5634407
cn7
Cdh1tm2Kem/Cdh1+
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N MGI:5634406
cn8
Cdh1tm1Kem/Cdh1tm2Kem
Tg(MMTV-cre)1Kem/0
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6 MGI:3697584
cn9
Cdh1tm2Kem/Cdh1tm2Kem
Tg(KRT14-cre)1Efu/0
Tg(Krt14-RNAi:Cdh3)1Efu/0
involves: 129S1/Sv * 129X1/SvJ MGI:3830547
cn10
Cdh1tm2Kem/Cdh1tm2Kem
Tg(Ins2-cre)23Herr/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CBA/J MGI:5494970


Genotype
MGI:3697488
ht1
Allelic
Composition
Cdh1tm2Kem/Cdh1tm5(Cdh2)Kem
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Cdh1tm5(Cdh2)Kem mutation (0 available); any Cdh1 mutation (166 available)
phenotype observed in females
phenotype observed in males
N normal phenotype



Genotype
MGI:5634401
cn2
Allelic
Composition
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Vil1-cre)20Syr/0
Genetic
Background
involves: 129 * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Smad4tm2.1Cxd mutation (2 available); any Smad4 mutation (33 available)
Tg(Vil1-cre)20Syr mutation (3 available)
Trp53tm1Brn mutation (17 available); any Trp53 mutation (205 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• mice develop intestinal adenocarcinomas with a median tumor-free survival of 5.2 months of age and no distant metastases are seen

neoplasm
• mice develop intestinal adenocarcinomas with a median tumor-free survival of 5.2 months of age and no distant metastases are seen




Genotype
MGI:4822000
cn3
Allelic
Composition
Cdh1tm1.1Mpst/Cdh1tm2Kem
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Tg(Vil1-cre)20Syr/0
Genetic
Background
involves: 129 * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm1.1Mpst mutation (0 available); any Cdh1 mutation (166 available)
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Gt(ROSA)26Sortm1Sor mutation (8 available); any Gt(ROSA)26Sor mutation (745 available)
Tg(Vil1-cre)20Syr mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype




Genotype
MGI:5634403
cn4
Allelic
Composition
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(MMTV-cre)7Mul/0
Genetic
Background
involves: 129 * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Smad4tm2.1Cxd mutation (2 available); any Smad4 mutation (33 available)
Tg(MMTV-cre)7Mul mutation (0 available)
Trp53tm1Brn mutation (17 available); any Trp53 mutation (205 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
integument
• mice develop mammary gland carcinoma with a median tumor-free survival of 10.4 months of age
• tumors are invasive ductal carcinomas with a squamous component, however lobular carcinomas are not seen

endocrine/exocrine glands
• mice develop mammary gland carcinoma with a median tumor-free survival of 10.4 months of age
• tumors are invasive ductal carcinomas with a squamous component, however lobular carcinomas are not seen

neoplasm
• mice develop mammary gland carcinoma with a median tumor-free survival of 10.4 months of age
• tumors are invasive ductal carcinomas with a squamous component, however lobular carcinomas are not seen
• 33% of mice show lung metastasis




Genotype
MGI:5634400
cn5
Allelic
Composition
Cdh1tm2Kem/Cdh1+
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
Genetic
Background
involves: 129 * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Smad4tm2.1Cxd mutation (2 available); any Smad4 mutation (33 available)
Tg(Pdx1-cre)6Tuv mutation (3 available)
Trp53tm1Brn mutation (17 available); any Trp53 mutation (205 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• 36% of mice develop duodenal adenocarcinomas
• 84% of mice develop spontaneous tumors in the glandular stomach with a median tumor-free survival of 8 months
• gastric tumors resemble diffuse-type gastric adenocarcinomas, are E-cadherin-negative, and are invasive into the muscle layers and regional lymph nodes
• intramucosal adenocarcinomas with signet ring cell feature is seen in 2 of 4 mice at 6 months of age
• gastric premalignant lesions such as atrophic gastritis, metaplasia or dysplasia are not seen at 4 and 5 months of age

mortality/aging
• most common cause of death is duodenal obstruction, followed by gastric outlet obstruction

neoplasm
• 36% of mice develop duodenal adenocarcinomas
• 84% of mice develop spontaneous tumors in the glandular stomach with a median tumor-free survival of 8 months
• gastric tumors resemble diffuse-type gastric adenocarcinomas, are E-cadherin-negative, and are invasive into the muscle layers and regional lymph nodes
• intramucosal adenocarcinomas with signet ring cell feature is seen in 2 of 4 mice at 6 months of age
• gastric premalignant lesions such as atrophic gastritis, metaplasia or dysplasia are not seen at 4 and 5 months of age
• 3 of 21 mice with gastric adenocarcinomas develop lung metastases
• metastatic lesions have similar cytologic features to primary gastric tumors
• 8% of mice exhibit adenocarcinomas in the pancreas, most likely due to invasion of the primary duodenal or gastric adenocarcinomas
• 24% of mice develop forestomach squamous cell carcinomas

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
stomach cancer DOID:10534 OMIM:613659
J:212549




Genotype
MGI:5634407
cn6
Allelic
Composition
Cdh1tm2Kem/Cdh1tm2Kem
Smad4tm2.1Cxd/Smad4tm2.1Cxd
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
Genetic
Background
involves: 129 * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Smad4tm2.1Cxd mutation (2 available); any Smad4 mutation (33 available)
Tg(Pdx1-cre)6Tuv mutation (3 available)
Trp53tm1Brn mutation (17 available); any Trp53 mutation (205 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
digestive/alimentary system
• mice develop gastric adenocarcinomas with a median tumor-free survival of 9.4 months but do not develop distant metastases

neoplasm
• mice develop gastric adenocarcinomas with a median tumor-free survival of 9.4 months but do not develop distant metastases




Genotype
MGI:5634406
cn7
Allelic
Composition
Cdh1tm2Kem/Cdh1+
Trp53tm1Brn/Trp53tm1Brn
Tg(Pdx1-cre)6Tuv/0
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Tg(Pdx1-cre)6Tuv mutation (3 available)
Trp53tm1Brn mutation (17 available); any Trp53 mutation (205 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
N
• mice do not develop gastric adenocarcinomas




Genotype
MGI:3697584
cn8
Allelic
Composition
Cdh1tm1Kem/Cdh1tm2Kem
Tg(MMTV-cre)1Kem/0
Genetic
Background
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm1Kem mutation (1 available); any Cdh1 mutation (166 available)
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Tg(MMTV-cre)1Kem mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• on day 16 of pregnancy, mammary glands of pregnant mice show only occasional and small units of secretion compared to wild-type mice; this becomes more striking by day 18 and at parturition
• mammary gland is disorganized, with poorly developed, condensed alveoli at parturition

endocrine/exocrine glands
• at day 2 of lactation, majority of lobulo-alveolar structures have collapsed, leaving clusters of epithelial cords with small lumina and extensive tissue remodeling
• on day 16 of pregnancy, mammary glands of pregnant mice show only occasional and small units of secretion compared to wild-type mice; this becomes more striking by day 18 and at parturition
• mammary gland is disorganized, with poorly developed, condensed alveoli at parturition
• expression of B-casein and whey acidic protein (WAP) re drastically reduced in mammary glands during lactation, indicating that gland is inefficient at producing milk proteins
• mutant mice cannot nurse their offspring due to mammary gland defects; 80% of offspring die within 24 hours and all die within 2-3 days of birth

integument
• at day 2 of lactation, majority of lobulo-alveolar structures have collapsed, leaving clusters of epithelial cords with small lumina and extensive tissue remodeling
• on day 16 of pregnancy, mammary glands of pregnant mice show only occasional and small units of secretion compared to wild-type mice; this becomes more striking by day 18 and at parturition
• mammary gland is disorganized, with poorly developed, condensed alveoli at parturition
• expression of B-casein and whey acidic protein (WAP) re drastically reduced in mammary glands during lactation, indicating that gland is inefficient at producing milk proteins
• mutant mice cannot nurse their offspring due to mammary gland defects; 80% of offspring die within 24 hours and all die within 2-3 days of birth




Genotype
MGI:3830547
cn9
Allelic
Composition
Cdh1tm2Kem/Cdh1tm2Kem
Tg(KRT14-cre)1Efu/0
Tg(Krt14-RNAi:Cdh3)1Efu/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Tg(KRT14-cre)1Efu mutation (0 available)
Tg(Krt14-RNAi:Cdh3)1Efu mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die within 1 to 2 hours of birth

growth/size/body
• mice are small at birth

homeostasis/metabolism
• unlike in wild-type mice, dye is readily absorbed through the paws, facial skin, ear buds and lower belly

integument
• mice exhibit focal gaps in the epithelial sheets of the epidermis due to degeneration of cells
• however, desmosomes are still present in normal numbers
• intercellular junctions are perturbed unlike in wild-type epidermis
• adherence and tight junction components fail to localize to cell borders unlike in wild-type epidermis
• the typically columnar orientation of cells within the basal layer and flattened squamous morphology of the suprabasal cells are lost
• cells in the spinous layer fail to flatten as in wild-type mice
• the typically columnar orientation of cells within the basal layer and flattened squamous morphology of the suprabasal cells are lost
• unlike in wild-type mice, cells within condensed nuclei indicating apoptosis are found in the suprabasal layer
• suprabasal keratin intermediate filament organization is perturbed compared to in wild-type mice
• around the mouth, umbilicus and tail
• ventrally
• skin is inflexible
• mice exhibit increased apoptosis in the epidermis compared to wild-type mice
• however, cell proliferation in the epidermis is normal, and no inflammatory response is observed
• unlike in wild-type mice, dye is readily absorbed through the paws, facial skin, ear buds and lower belly




Genotype
MGI:5494970
cn10
Allelic
Composition
Cdh1tm2Kem/Cdh1tm2Kem
Tg(Ins2-cre)23Herr/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CBA/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdh1tm2Kem mutation (1 available); any Cdh1 mutation (166 available)
Tg(Ins2-cre)23Herr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• in 7 day and 1, but not 3, month old mice
• 2-fold increase in blood vessel density in insulin-positive areas
• however, beta cell organization is normal
• at 3 months
• 2.5-fold at 3 to 4 months
• glucose-stimulated at 4, but not 1, months

homeostasis/metabolism
• glucose-stimulated at 4, but not 1, months
• at 3 to 4 months

cardiovascular system
• 2-fold increase in blood vessel density in insulin-positive areas

growth/size/body
N
• mice exhibit normal body weight

cellular
• in 7 day and 1, but not 3, month old mice





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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory