Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
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immune system
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• after immunization with ovalbumin, severe deficit in IgE is observed with little or no Ig E detected
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• NK cells exhibit severely decreased killing of hematopoietic cells RMA-S, YAC-1 and MHC class I-deficient splenocytes in vitro compared with wild-type cells
• mice exhibit a 10-fold increase in residual RMA-S cells in the peritoneal cavity compared with wild-type mice
• when 2B4 is engaged, the activating effect is inhibited compared to on wild-type cells
• NK cells exhibit reduced conjugation with RMA-S cells compared with wild-type cells
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• pronounced defect in production of IL-4 upon stimulation with anti-CD3 or anti-CD3 plus anti-CD28
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• in response to RMA-S and YAC-1 cells, more severe than in Sh2d1atm1Vei homozygotes
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hematopoietic system
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• after immunization with ovalbumin, severe deficit in IgE is observed with little or no Ig E detected
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• NK cells exhibit severely decreased killing of hematopoietic cells RMA-S, YAC-1 and MHC class I-deficient splenocytes in vitro compared with wild-type cells
• mice exhibit a 10-fold increase in residual RMA-S cells in the peritoneal cavity compared with wild-type mice
• when 2B4 is engaged, the activating effect is inhibited compared to on wild-type cells
• NK cells exhibit reduced conjugation with RMA-S cells compared with wild-type cells
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• pronounced defect in production of IL-4 upon stimulation with anti-CD3 or anti-CD3 plus anti-CD28
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Allelic
Composition |
Sh2d1atm1Pls/Sh2d1atm1Pls
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Genetic
Background |
either: 129S6/SvEvTac or (involves: 129S6/SvEvTac * C57BL/6) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
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cellular
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• tendency for T-cell proliferation to be increased even in the absence of infection
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hematopoietic system
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• tendency for T-cell proliferation to be increased even in the absence of infection
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• although lymphocyte numbers were generally normal, occasional animals had increased T cell and NK cell numbers together with decreased B cell numbers in the spleen
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• biased toward the production of the Th1 cytokines Il-2 and IFN-gamma
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• biased toward decreased production of the Th2 cytokine Il-4
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• although levels of IgM, IgG, and IgA were normal, IgE levels were reduced
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immune system
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• tendency for T-cell proliferation to be increased even in the absence of infection
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• although lymphocyte numbers were generally normal, occasional animals had increased T cell and NK cell numbers together with decreased B cell numbers in the spleen
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• biased toward the production of the Th1 cytokines Il-2 and IFN-gamma
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• biased toward decreased production of the Th2 cytokine Il-4
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• although levels of IgM, IgG, and IgA were normal, IgE levels were reduced
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• elevated T-cell response to Toxoplasma gondii infection
• lower titers of Ig isotypes directed against Toxoplasma antigens
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• increased T-cell response to infection with lymphocyte choriomeningitis virus (LCMV)
• Elevated activity of CD8+ cytotoxic T lymphocytes in spleen
• CD4+ cells also increased 2-3X
• decrease in antibody secreting cells in spleen and bone marrow
• more virulent strains led to increased morbidity
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
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immune system
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• 7-9 days after immunization , there is a clear decrease in CD4+ T cells expressing Tfh cell markers
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• fewer 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibody-secreting cells in the bone marrow 30 days post immunization
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• invariant NKT cells exhibit impaired microtubule-organizing center polarization compared with wild-type cells
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• high-affinity 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibodies are at lower titers than in wild-type mice, 30 days postimmunization
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• adhesion to plastic surfaces coated with CD84 or Ly108 is impaired
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• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, inhibition of SHP rescues adhesion
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• from mice immunized with the T-dependent antigen 4-hydroxy-3-nitrophenyl acetyl(NP) -OVA
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hematopoietic system
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• 7-9 days after immunization , there is a clear decrease in CD4+ T cells expressing Tfh cell markers
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• fewer 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibody-secreting cells in the bone marrow 30 days post immunization
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• invariant NKT cells exhibit impaired microtubule-organizing center polarization compared with wild-type cells
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• high-affinity 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibodies are at lower titers than in wild-type mice, 30 days postimmunization
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• adhesion to plastic surfaces coated with CD84 or Ly108 is impaired
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• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, inhibition of SHP rescues adhesion
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
|
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immune system
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N |
• natural killer cell number and function are normal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
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immune system
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• homozygous mutants on a BALB/c background develop smaller lesions following infection with L. major
• no difference in response is seen on a C57BL/6 background
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immune system
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N |
• mice exhibit normal response to LCMV infection
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
Tg(TcraTcrb)425Cbn mutation
(3 available)
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immune system
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• T cell to B cell antigen specific, dose dependent conjugate formation is maintained for 10 min but not for 30 min
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hematopoietic system
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• T cell to B cell antigen specific, dose dependent conjugate formation is maintained for 10 min but not for 30 min
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
Sh2d1b1tm3Vei mutation
(0 available);
any
Sh2d1b1 mutation
(8 available)
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immune system
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• decreased killing of RMA-S
• reduced ability to convert 2B4 into an inhibitory receptor
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hematopoietic system
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• decreased killing of RMA-S
• reduced ability to convert 2B4 into an inhibitory receptor
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
Tg(TcraTcrb)1100Mjb mutation
(6 available)
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immune system
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• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, T cells exhibit increased adhesion to and normal killing of B cells lacking Slamf6 (Sh2d1atm1Pls)
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• in cytotoxic T cells stimulated with ovalbumin
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hematopoietic system
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• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, T cells exhibit increased adhesion to and normal killing of B cells lacking Slamf6 (Sh2d1atm1Pls)
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immune system
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N |
• T cells exhibit normal adhesion to wild-type B cells and B cells lacking Slamf6 (Sh2d1atm1Pls)
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|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
Tg(DO11.10)10Dlo mutation
(7 available)
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immune system
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• CD4+ T cells produce less IL-4 following treatment with OVA peptide-pulsed antigen presenting cells
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Allelic
Composition |
Sh2d1atm1Pls/Y
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|
Genetic
Background |
involves: 129S6/SvEvTac |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sh2d1atm1Pls mutation
(4 available);
any
Sh2d1a mutation
(10 available)
|
|
|
immune system
 |
• 7-9 days after immunization , there is a clear decrease in CD4+ T cells expressing Tfh cell markers
|
|
|
• fewer 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibody-secreting cells in the bone marrow 30 days post immunization
|
|
|
• invariant NKT cells exhibit impaired microtubule-organizing center polarization compared with wild-type cells
|
|
|
• high-affinity 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibodies are at lower titers than in wild-type mice, 30 days postimmunization
|
|
|
• adhesion to plastic surfaces coated with CD84 or Ly108 is impaired
|
|
|
• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, inhibition of SHP rescues adhesion
|
|
|
• from mice immunized with the T-dependent antigen 4-hydroxy-3-nitrophenyl acetyl(NP) -OVA
|
hematopoietic system
|
|
• 7-9 days after immunization , there is a clear decrease in CD4+ T cells expressing Tfh cell markers
|
|
|
• fewer 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibody-secreting cells in the bone marrow 30 days post immunization
|
|
|
• invariant NKT cells exhibit impaired microtubule-organizing center polarization compared with wild-type cells
|
|
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• high-affinity 4-hydroxy-3-nitrophenyl acetyl(NP) specific antibodies are at lower titers than in wild-type mice, 30 days postimmunization
|
|
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• adhesion to plastic surfaces coated with CD84 or Ly108 is impaired
|
|
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• impaired adhesion to wild-type B cells when stimulated with ovalbumin
• however, inhibition of SHP rescues adhesion
|
Analysis Tools