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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Raf1tm1Bacc
targeted mutation 1, Manuela Baccarini
MGI:2388026
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Raf1tm1Bacc/Raf1tm1Bacc involves: 129P2/OlaHsd * 129/Sv MGI:3042138
hm2
Raf1tm1Bacc/Raf1tm1Bacc involves: 129P2/OlaHsd * C57BL/6 MGI:3042139
cn3
Braftm1Sva/Braftm1.1Sva
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713581
cn4
Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713654
cx5
Faslpr/Fas+
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL MGI:3799534
cx6
Faslpr/Faslpr
Raf1tm1Bacc/Raf1tm1Bacc
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL MGI:3799535
cx7
Faslpr/Fas+
Raf1tm1Bacc/Raf1+
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL MGI:3799536


Genotype
MGI:3042138
hm1
Allelic
Composition
Raf1tm1Bacc/Raf1tm1Bacc
Genetic
Background
involves: 129P2/OlaHsd * 129/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes show a progressive decline in survival between E11.5 and E13.5, with none surviving beyond E13.5
• Background Sensitivity: on a mixed background involving 129 and C57BL/6, homozygotes display a broader window of lethality with none surviving past E16.5

embryo
• starting at E11.5, homozygous mutant embryos appear developmentally retarded relative to wild-type or heterozygous controls
• at E12.5, homozygous mutant embryos are smaller than normal
• homozygous mutant embryos display a ~20% weight reduction by E12.5 relative to controls embryos
• at E12.5, the mutant spongiotrophoblast layer is reduced in size relative to that in wild-type controls
• at E12.5, the mutant labyrinth layer is reduced in size and contains numerous mesenchymal cells relative to that in wild-type controls
• at E12.5, the mutant labyrinth layer is poorly vascularized
• in contrast, the decidua appears normal
• at E12.5, mutant placentas are smaller than normal
• mutant placentas show a ~20% weight reduction by E12.5 relative to control placentas

growth/size/body
• starting at E11.5, homozygous mutant embryos appear developmentally retarded relative to wild-type or heterozygous controls
• at E12.5, homozygous mutant embryos are smaller than normal
• homozygous mutant embryos display a ~20% weight reduction by E12.5 relative to controls embryos

liver/biliary system
• at E12.5, mutant liver sinuses contain fewer red blood cells than wild-type
• at E12.5, mutant liver cells appear slightly enlarged relative to wild-type cells
• at E12.5, mutant livers are significantly smaller than wild-type
• at E12.5, mutant livers are hypocellular relative to control livers
• however, no defect in hepatoblast proliferation is observed, as determined by PCNA staining
• at E12.5, mutant livers are very pale
• at E12.5, most mutant hepatoblasts (but not erythroid cells) exhibit numerous pyknotic and fragmented nuclei, as shown by immunohistochemical staining
• at E12.5, TUNEL analysis of mutant livers indicates an increased level of apoptosis mainly in the hepatoblast compartment

cardiovascular system
• at E12.5, mutant liver sinuses contain fewer red blood cells than wild-type
• at E12.5, the mutant labyrinth layer is poorly vascularized
• in contrast, the decidua appears normal

cellular
• in culture, primary MEFs established from E11.5-E12.5 mutant embryos exhibit a >4-fold increase in apoptosis relative to wild-type MEFs, as shown by TUNEL staining
• in contrast, the number of S-phase MEFs is similar, arguing against a cell cycle defect
• mutant MEFs immortalized according to the 3T3 protocol lose their hypersensitivity towards actinomycin D-induced apoptosis
• 3T3-like mutant cells are resistant to the cytotoxic effects of TNF alone, but are as susceptible as wild type cells to a combination of TNF plus cycloheximide
• primary MEFs established from E11.5-E12.5 mutant embryos are more susceptible than wild type to apoptosis induced by growth factor deprivation and actinomycin D treatment
• mutant MEFs immortalized according to the 3T3 protocol are more susceptible than wild-type cells to Fas activation

integument
• at E12.5, mutant embryos are paler than normal




Genotype
MGI:3042139
hm2
Allelic
Composition
Raf1tm1Bacc/Raf1tm1Bacc
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• on a mixed genetic background, homozygotes display a progressive decline in survival between E11.5 and E16.5; no homozygotes are recovered past E16.5
• Background Sensitivity: on an inbred 129 background, homozygotes die between E11.5 and E13.5

embryo
• starting at E11.5, homozygous mutant embryos appear developmentally retarded relative to wild-type or heterozygous controls
• at E12.5, homozygous mutant embryos are smaller than normal
• homozygous mutant embryos display a ~20% weight reduction by E12.5 relative to controls embryos
• at E12.5, the mutant spongiotrophoblast layer is reduced in size relative to that in wild-type controls
• at E12.5, the mutant labyrinth layer is reduced in size and contains numerous mesenchymal cells relative to that in wild-type controls
• at E12.5, the mutant labyrinth layer is poorly vascularized
• in contrast, the decidua appears normal
• at E12.5, mutant placentas are smaller than normal
• mutant placentas show a ~20% weight reduction by E12.5 relative to control placentas

growth/size/body
• starting at E11.5, homozygous mutant embryos appear developmentally retarded relative to wild-type or heterozygous controls
• at E12.5, homozygous mutant embryos are smaller than normal
• homozygous mutant embryos display a ~20% weight reduction by E12.5 relative to controls embryos

liver/biliary system
• at E12.5, mutant liver sinuses contain fewer red blood cells than wild-type
• at E12.5, mutant liver cells appear slightly enlarged relative to wild-type cells
• at E12.5, mutant livers are significantly smaller than wild-type
• at E12.5, mutant livers are hypocellular relative to control livers
• however, no defect in hepatoblast proliferation is observed, as determined by PCNA staining
• at E12.5, mutant livers are very pale
• at E12.5, most mutant hepatoblasts (but not erythroid cells) exhibit numerous pyknotic and fragmented nuclei, as shown by immunohistochemical staining
• at E12.5, TUNEL analysis of mutant livers revealed an increased level of apoptosis mainly in the hepatoblast compartment

cardiovascular system
• at E12.5, mutant liver sinuses contain fewer red blood cells than wild-type
• at E12.5, the mutant labyrinth layer is poorly vascularized
• in contrast, the decidua appears normal

cellular
• in asynchronous cultures, multipotent hematopoietic precursors established from E11.5 mutant livers display a >5-fold increase in spontaneous apoptosis relative to wild-type hematopoietic cells, as shown by annexin V staining
• no significant defects in proliferation of mutant fetal liver cells are observed in vitro
• in culture, primary MEFs established from E11.5-E12.5 mutant embryos exhibit a >4-fold increase in apoptosis relative to wild-type MEFs, as shown by TUNEL staining; in contrast, the number of S-phase MEFs is similar, arguing against a cell cycle defect `
• mutant MEFs immortalized according to the 3T3 protocol lose their hypersensitivity towards actinomycin D-induced apoptosis
• 3T3-like mutant cells are resistant to the cytotoxic effects of TNF alone, but are as susceptible as wild type cells to a combination of TNF plus cycloheximide
• primary MEFs established from E11.5-E12.5 mutant embryos are more susceptible than wild type to apoptosis induced by growth factor deprivation and actinomycin D treatment
• mutant MEFs immortalized according to the 3T3 protocol are more susceptible than wild-type cells to Fas activation

integument
• at E12.5, mutant embryos are paler than normal




Genotype
MGI:3713581
cn3
Allelic
Composition
Braftm1Sva/Braftm1.1Sva
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Sva mutation (0 available); any Braf mutation (38 available)
Braftm1Sva mutation (1 available); any Braf mutation (38 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (102 available)
Tg(Nes-cre)1Kln mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no double conditional embryos are found live at E14-15, but can be isolated at E13

nervous system
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
• at E13, Ret levels in DRGs are reduced

cellular
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth




Genotype
MGI:3713654
cn4
Allelic
Composition
Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (38 available)
Braftm1Wds mutation (0 available); any Braf mutation (38 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
Raf1tm2Bacc mutation (0 available); any Raf1 mutation (102 available)
Tg(Nes-cre)1Kln mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no double conditional embryos are found live at E14-15, but can be isolated at E13

nervous system
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
• at E13, Ret levels in DRGs are reduced

cellular
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth




Genotype
MGI:3799534
cx5
Allelic
Composition
Faslpr/Fas+
Raf1tm1Bacc/Raf1tm1Bacc
Genetic
Background
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Faslpr mutation (25 available); any Fas mutation (55 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• introduction of a Faslpr allele onto the Raf1-null background rescues the lethality shown by the homozygous Raf1-null embryos

hematopoietic system
N
• introduction of a Faslpr allele onto the Raf1-null background rescues the anemia observed in homozygous Raf1-null embryos
• differentiation of erythroblasts is delayed, restoring differentiation to level of Raf1 heterozygotes; differentiation is slower than observed in Raf1-null cells




Genotype
MGI:3799535
cx6
Allelic
Composition
Faslpr/Faslpr
Raf1tm1Bacc/Raf1tm1Bacc
Genetic
Background
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Faslpr mutation (25 available); any Fas mutation (55 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• differentiation of erythroblasts is blocked even at 60 hours after induction of differentiation, as measured by cell proliferation, volume decrease, and hemoglobin accumulation

cellular
• number of apoptotic erthroblasts is increased under 10U/ml of erythropoietin




Genotype
MGI:3799536
cx7
Allelic
Composition
Faslpr/Fas+
Raf1tm1Bacc/Raf1+
Genetic
Background
involves: 129/SvHsd * 129P2/OlaHsd * C57BL/6 * MRL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Faslpr mutation (25 available); any Fas mutation (55 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (102 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
N
• differentiation of erythroblasts from double heterozygotes is indistinguishable from wild-type cells





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last database update
12/05/2017
MGI 6.11
The Jackson Laboratory