Mouse Genome Informatics
cn1
    Ogttm1Gwh/Y
involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• recombined embryonic stem cells are found at 24 and 48 h following cre transduction but by 144 h all viable cells carry the unrecombined allele (J:62279)
• primary embryonic fibroblasts infected with viral cre senesce and die by 12 days after infection (J:93112)
• primary embryonic fibroblasts infected with viral cre do not form colonies of more than 10 cells indicating a failure to undergo 4 or more cell divisions


Mouse Genome Informatics
cn2
    Ogttm1Gwh/Ogt+
Tg(Prm-cre)58Og/0

involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice are viable and fertile with no gross abnormalities and expression levels of Ogt that are similar to controls suggesting inactivation of the paternal X chromosome in all tissues and cell types surveyed
• mice do not produce any offspring carrying the recombined allele


Mouse Genome Informatics
cn3
    Ogttm1Gwh/Ogttm1Gwh
Tg(Zp3-cre)1Gwh/0

involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• when the cre is inherited maternally, no mice carrying the recombined allele are detected

reproductive system
• when the cre is inherited paternally mice survive but females are infertile


Mouse Genome Informatics
cn4
    Ogttm1Gwh/Ogt+
Tg(Zp3-cre)1Gwh/0

involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no mice carrying the recombined allele are detected


Mouse Genome Informatics
cn5
    Ogttm1Gwh/Y
Tg(Zp3-cre)1Gwh/0

involves: 129S1/Sv * 129X1/SvJ
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no mice carrying the recombined allele are detected


Mouse Genome Informatics
cn6
    Ogttm1Gwh/Y
Tg(Lck-cre)548Jxm/0

involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• increase in annexin-V expression in single positive T cells
• about a 75% reduction in the number of T cells in the lymph nodes and spleen
• no T cells carrying the recombined allele are found in the lymph nodes or spleen
• about a 50% reduction in thymocyte cell number
• cortical double positive T cells are reduced by 50%

hematopoietic system
• increase in annexin-V expression in single positive T cells
• about a 75% reduction in the number of T cells in the lymph nodes and spleen
• no T cells carrying the recombined allele are found in the lymph nodes or spleen
• about a 50% reduction in thymocyte cell number
• cortical double positive T cells are reduced by 50%

cellular
• increase in annexin-V expression in single positive T cells

endocrine/exocrine glands
• about a 50% reduction in thymocyte cell number


Mouse Genome Informatics
cn7
    Ogttm1Gwh/Y
Tg(Syn1-cre)671Jxm/0

involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• none survive for more than 10 days
• found at only about 50% of the expected frequency

behavior/neurological
• rarely nurse
• fail to develop normal locomotor activity

growth/size/body


Mouse Genome Informatics
cn8
    Ogttm1Gwh/Ogttm1Gwh
Tg(Syn1-cre)671Jxm/0

involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Key:
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• none survive for more than 10 days

behavior/neurological
• rarely nurse
• fail to develop normal locomotor activity

growth/size/body