Phenotypes associated with this allele
Allelic
Composition |
Rad50tm2Jpt/Rad50tm2Jpt
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Genetic
Background |
either: (involves: 129S6/SvEvTac * 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
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• mean age of death is 2.6 months +/- 1.8 months
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• partial embryonic lethality at day E14-E16
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neoplasm
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• 20% of animals displayed metastatic thymic lymphomas at 4-7 months of age
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• 1 animal displayed myeloid leukemia
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growth/size/body
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• weight of mutant mice was 60% of controls
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• 4 animals displayed splenic hyperplasia
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hematopoietic system
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• abnormal thymus architecture
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• at 1 week of age mutant mice displayed a 2-3 fold increase in pro B cells
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• by 4-8 weeks of age animals displayed gray and wasted appearance
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• at 4 weeks of age mutant mice displayed severe depletion of lymphocytes, macrophages, red blood cells, and platelets
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• bone marrow devoid of hematopoietic cells and composed largely of adipocytes
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• splenic B cell count at 4.8%-12% of wild-type
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• at 1 week of age mutant mice displayed a 1.5-3 fold reduction in immature B cell population
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• at 1 week of age mutant mice displayed a 1.5-3 fold reduction in pre B cell population
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• splenic T cell count at 7%-49% of wild-type
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• at 1 week of age mutant mice displayed a 2-3 fold increase in macrophages
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• abnormal spleen architecture
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• 4 animals displayed splenic hyperplasia
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immune system
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• at 1 week of age mutant mice displayed a 2-3 fold increase in pro B cells
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• splenic B cell count at 4.8%-12% of wild-type
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• at 1 week of age mutant mice displayed a 1.5-3 fold reduction in immature B cell population
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• at 1 week of age mutant mice displayed a 1.5-3 fold reduction in pre B cell population
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• splenic T cell count at 7%-49% of wild-type
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• at 1 week of age mutant mice displayed a 2-3 fold increase in macrophages
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• abnormal thymus architecture
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• abnormal spleen architecture
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• 4 animals displayed splenic hyperplasia
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endocrine/exocrine glands
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• abnormal thymus architecture
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• 20% of animals displayed metastatic thymic lymphomas at 4-7 months of age
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• 97.5% of mice succumb to anemia at 8 months
|
hematopoietic system
|
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• most die during the first 5 months of age
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hematopoietic system
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• decrease in pro-B cell numbers
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• 82 of 83 die with anemia
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• increase in apoptosis in the bone marrow as indicated by increased levels of cleaved Caspase-3
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• peripheral blood cell numbers are decreased (32% vs. 41% in wild-type)
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immune system
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• decrease in pro-B cell numbers
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neoplasm
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• 1 of 83 die with lymphoma
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation
(1 available);
any
Rad50 mutation
(53 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• 42% die by 5 months of age, however survival is increased compared to homozygous Rad50tm2Jpt mice
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hematopoietic system
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• 12 of 29 die with anemia
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• most die from hematopoietic failure
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neoplasm
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• 3 of 29 die with lymphoma
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek2tm1Mak mutation
(1 available);
any
Chek2 mutation
(53 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• 13 of 65 die by 5 months of age, however survival time is increased compared to homozygous Rad50tm2Jpt mice, as 52 mutants survive past 5 months of age and none develop anemia
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neoplasm
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• 10 of 65 mice develop lymphoma
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hematopoietic system
|
N |
• exhibit hematopoietic rescue of the abnormalities seen in homozygous Rad50tm2Jpt mice, with numbers of double-negative T cells, pro-B cells, and macrophages within 2-fold levels of wild-type levels
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek2tm1Mak mutation
(1 available);
any
Chek2 mutation
(53 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• 31 of 41 die by 5 months of age
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hematopoietic system
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• pro-B cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• double-negative T cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• 36 of 41 develop anemia
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• macrophage numbers are similar to that in homozygous Rad50tm2Jpt mice
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immune system
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• pro-B cell numbers are similar to that in homozygous Rad50tm2Jpt mice
|
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• double-negative T cell numbers are similar to that in homozygous Rad50tm2Jpt mice
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• macrophage numbers are similar to that in homozygous Rad50tm2Jpt mice
|
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
Smc1atm1Mbk mutation
(1 available);
any
Smc1a mutation
(10 available)
|
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mortality/aging
 |
• double homozygotes are indistinguishable from homozygous Rad50tm2Jpt mice with respect to survival and die within 5 months after birth
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hematopoietic system
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• double homozygotes are indistinguishable from homozygous Rad50 mice with respect to hematopoietic cell numbers
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• pro-B cell numbers are similar to that in homozygous Rad50 mice
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• double-negative T cell numbers are similar to that in homozygous Rad50 mice
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• 26 of 27 develop anemia
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• macrophage numbers are similar to that in homozygous Rad50 mice
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immune system
|
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• pro-B cell numbers are similar to that in homozygous Rad50 mice
|
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• double-negative T cell numbers are similar to that in homozygous Rad50 mice
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• macrophage numbers are similar to that in homozygous Rad50 mice
|
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|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
Trp53tm1Tyj mutation
(12 available);
any
Trp53 mutation
(232 available)
|
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mortality/aging
|
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• mean age of death is 4.5 months
|
neoplasm
immune system
|
N |
• B cell numbers increased 5-20 fold compared to Rad50tm2Jpt homozygous mice
|
reproductive system
 |
• suppression of testicular apoptosis
• normal meiotic progression
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endocrine/exocrine glands
|
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• suppression of testicular apoptosis
• normal meiotic progression
|
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|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atmtm1Awb mutation
(7 available);
any
Atm mutation
(169 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
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• although most succumb to anemia, they have a significantly greater survival time than homozygous Rad50tm2Jpt mice, with 85% living longer than 5 months of age
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neoplasm
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• 16 of 67 develop lymphoma
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hematopoietic system
|
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• macrophage counts are decreased to a similar level as in homozygous Atmtm1Awb mice
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immune system
|
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• macrophage counts are decreased to a similar level as in homozygous Atmtm1Awb mice
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atmtm1Awb mutation
(7 available);
any
Atm mutation
(169 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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mortality/aging
|
|
• although die of malignancy, they have a significantly greater survival time than homozygous Rad50tm2Jpt mice, with 42% living longer than 5 months, 20.5% surviving to 10 months and 18% surviving to 15 months
|
neoplasm
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• although mutants develop lymphomas, the latency of lymphomagenesis is increased compared to homozygous Atmtm1Awb mice
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cellular
|
N |
• growth of MEFs and sensitivity to irradiation is comparable to wild-type indicating rescue of the slower growth of MEFs and increased sensitivity to gamma-irradiation that is seen in homozygous Atmtm1Awb mice
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• MEFs exhibit defects in G1/S, intra-S-phase, and G2/M checkpoints after ionizing radiation induced DNA damage
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• exhibit some chromosomal instability, however it is slightly reduced relative to homozygous Atm mice
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hematopoietic system
|
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbntm2.1Jpt mutation
(0 available);
any
Nbn mutation
(59 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
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|
mortality/aging
|
N |
• unlike Rad50tm2Jpt homozygotes, mice do not exhibit increased lethality or anemia at 8 months
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cellular
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• attrition of B-, T- and myeloid cell lineages, gut and seminiferous tubules observed in Rad50tm2Jpt homozygotes is mitigated
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hematopoietic system
|
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• compared to Rad50tm2Jpt homozygotes, the age of onset of anemia is reduced
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbntm2.1Jpt mutation
(0 available);
any
Nbn mutation
(59 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
|
|
mortality/aging
|
N |
• unlike Rad50tm2Jpt homozygotes, mice do not exhibit increased lethality or anemia at 8 months
|
cellular
|
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• attrition of B-, T- and myeloid cell lineages, gut and seminiferous tubules observed in Rad50tm2Jpt homozygotes is mitigated
|
hematopoietic system
|
|
• compared to Rad50tm2Jpt homozygotes, the age of onset of anemia is reduced
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbntm1Jpt mutation
(1 available);
any
Nbn mutation
(59 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
|
|
hematopoietic system
|
N |
• exhibit hematopoietic rescue of the abnormalities seen in homozygous Rad50tm2Jpt mice, with numbers of double-negative T cells, pro-B cells, and macrophages within 5-fold levels of wild-type levels
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nbntm1Jpt mutation
(1 available);
any
Nbn mutation
(59 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
|
|
hematopoietic system
|
N |
• exhibit hematopoietic rescue of the abnormalities seen in homozygous Rad50tm2Jpt mice, with numbers of double-negative T cells, pro-B cells, and macrophages within 5-fold levels of wild-type levels
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mre11atm1Jpt mutation
(1 available);
any
Mre11a mutation
(48 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
|
|
mortality/aging
|
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• 32 of 49 die before 5 months of age, however survival time is increased compared to homozygous Rad50tm2Jpt mice, as 17 mutants survive past 5 months of age
|
hematopoietic system
|
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• 19 of the 32 mutants that die exhibited hematopoietic attrition
|
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• 19 of the 32 mutants that die exhibit anemia
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neoplasm
|
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• 10 of the 32 mutants that die succumb to lymphoma
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mre11atm1Jpt mutation
(1 available);
any
Mre11a mutation
(48 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
|
|
|
mortality/aging
|
|
• although some die prematurely, survival time is increased compared to homozygous Rad50tm2Jpt mice, with 24 of 29 alive at 5 months of age with no signs of anemia
|
neoplasm
|
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• 4 of 24 develop lymphoma
|
cellular
|
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• MEFs exhibit defects in G1/S, intra-S-phase, and G2/M checkpoints after ionizing radiation induced DNA damage
|
|
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• MEFs exhibit increased sensitivity to ionizing radiation
|
hematopoietic system
|
N |
• do not observe hematopoietic cell attrition as in homozygous Rad50 mice, indicating rescue of hematopoietic defects
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
Tg(BCL2)22Wehi mutation
(2 available)
|
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mortality/aging
|
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• although mutants succumb to anemia, it is significantly later than in homozygous Rad50tm2Jpt mice, with all dying by 7.5 months of age
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hematopoietic system
|
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• although mutants succumb to anemia, it is significantly later than in homozygous Rad50tm2Jpt mice
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Analysis Tools