Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation
(1 available);
any
Rad50 mutation
(53 available)
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Development of Rad50tm1Jpt/Rad50tm1Jpt embryos
mortality/aging
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• embryos become abnormal by E6 and are largely resorbed by E7.5 and E8.5
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growth/size/body
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• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5
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embryo
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• in the small E6 embryos, endodermal and ectodermal cells are packed loosely and appear not to form the normal columnar ectoderm
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• some E6.5 embryos have recognizable embryonic tissues but they are not developed appreciably and resemble E6 embryos both in size and degree of germ-layer differentiation
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• some embryos between E6 and E8.5 are significantly smaller than wild-type, however no morphological abnormalities are seen at E5.5
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cellular
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• blastocyst explants exhibit increased sensitivity to gamma-irradiation, with only the nonmitotic trophoblast giant cells remaining viable after irradiation
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• E6.5 embryos exhibit a decrease in the number of proliferating cells but no differences in the levels of apoptosis
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation
(1 available);
any
Rad50 mutation
(53 available)
Rad50tm4.1Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
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mortality/aging
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• no live mice are found at birth
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm1Jpt mutation
(1 available);
any
Rad50 mutation
(53 available)
Rad50tm2Jpt mutation
(0 available);
any
Rad50 mutation
(53 available)
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mortality/aging
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• 42% die by 5 months of age, however survival is increased compared to homozygous Rad50tm2Jpt mice
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hematopoietic system
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• 12 of 29 die with anemia
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• most die from hematopoietic failure
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neoplasm
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• 3 of 29 die with lymphoma
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mortality/aging
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• tamoxifen treated mice do not live beyond 2 weeks of age due to intestinal failure
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digestive/alimentary system
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• intestinal defects are seen by day 9 of tamoxifen treatment
• die of intestinal failure
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behavior/neurological
nervous system
N |
• mice exhibit normal nervous system morphology with no increase in DNA damage
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liver/biliary system
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• following partial hepatectomy, proliferation of pIpC-treated hepatocyte is decreased compared to in wild-type mice due to accumulation of DNA damage
• however, a subset of hepatocytes resolve their DNA damage and divide
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• while liver regeneration following partial hepatectomy is normal, pIpC-treated hepatocytes exhibit an increase in DNA damage compared to wild-type cells
• however, a subset of hepatocytes resolve their DNA damage and divide
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• following partial hepatectomy, pIpC-treated livers exhibit reduced cellularity and endoreduplication compared to in similarly-treated wild-type mice
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hematopoietic system
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• the only viable bone marrow left after treatment with pIpC fails to exhibit recombination
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cellular
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• following partial hepatectomy, proliferation of pIpC-treated hepatocyte is decreased compared to in wild-type mice due to accumulation of DNA damage
• however, a subset of hepatocytes resolve their DNA damage and divide
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