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Allele Symbol Allele Name Allele ID |
Mertktm1Gkm targeted mutation 1, Glenn K Matsushima MGI:2387768 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice injected with spleen cells from H2-Ab1
• when B cells are used in adoptive transfer into Rag knock-out mice that were then challenged with spleen cells from H2-Ab1
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• mice injected with spleen cells from H2-Ab1
• however, splenocytes from H2-Ab1
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 6 months, strands of nuclei from the inner nuclear layer begin to cross the inner plexiform layer towards the ganglion cell layer
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• at 6 months of age, retinal capillaries invade the retinal pigment epithelium unlike in wild-type mice
• at 8 months, retinal pigment epithelium invades the retina and localizes along retinal blood vessels unlike in wild-type mice
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• at P20, the rod outer segment is longer than in wild-type mice and results in a 67% greater concentration of rhodopsin than in wild-type mice
• by P60, all outer segment membranes and rhodopsin are undetectable unlike in wild-type mice
• unlike in wild-type retinas, vacuoles containing delicate fragments of outer segment membranes are observed
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• at P21
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• at later ages, a debris zone from the degenerating outer segment replaces the inner segment and outer segment layer
• however, by P45 the debris zone has largely disappeared
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• beyond P45, mice exhibit retinal rod cell degeneration unlike in wild-type mice
• however, no hemispheric difference in the degeneration is observed
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• early in degeneration, membranous whorls are present on the retinal pigment epithelium unlike in wild-type mice
• however, by P45 the whorls have largely disappeared
• few, if any, phagosome are found in the retinal pigment epithelium at the peak period of rod outer disc shedding unlike in wild-type mice
• at 6 months of age, retinal capillaries invade the retinal pigment epithelium unlike in wild-type mice
• at 8 months, retinal pigment epithelium invades the retina and localizes along retinal blood vessels unlike in wild-type
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• at P45, small focal thinning in the retinal pigment epithelium is observed unlike in wild-type mice
• however, some retinal pigment epithelium is thicker than normal
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• at P40, 70% of cells in the outer nuclear layer are pyknotic unlike in wild-type mice
• at P20, invading macrophages or microglia are observed in the outer nuclear layer unlike in wild-type mice
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• progressive thinning of the retinal outer nuclear layer (ONL) beginning at P25 with less than one row of cell nuclei remaining by P45
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• at a similar, if not slightly more rapid, rate of degeneration in pink-eyed RCS rats
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• at P33, photopic amplitudes are lower than normal
• at P33, photopic implicit time is delayed 75% compared to in wild-type mice
• photopic b wave amplitudes and implicit times decline rapidly with age
• no clear a or b waves are detected beyond P40 in some mice
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• at P30, scotopic a and b waves are lower than normal
• at P33, scotopic implicit time is delayed 75% compared to in wild-type mice
• scotopic a and b wave amplitudes and implicit times decline rapidly with age
• no clear a or b waves are detected beyond P40 in some mice
• at P33 and older, scotopic threshold response is higher than in wild-type mice
• however, scotopic threshold response is measurable at bright stimulus intensities when only scattered PR nuclei remain as late as P253
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• at 6 months of age, retinal capillaries invade the retinal pigment epithelium unlike in wild-type mice
• at 8 months, retinal pigment epithelium invades the retina and localizes along retinal blood vessels unlike in wild-type mice
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• at P20, the rod outer segment is longer than in wild-type mice and results in a 67% greater concentration of rhodopsin than in wild-type mice
• by P60, all outer segment membranes and rhodopsin are undetectable unlike in wild-type mice
• unlike in wild-type retinas, vacuoles containing delicate fragments of outer segment membranes are observed
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• at P21
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• at later ages, a debris zone from the degenerating outer segment replaces the inner segment and outer segment layer
• however, by P45 the debris zone has largely disappeared
|
|
• beyond P45, mice exhibit retinal rod cell degeneration unlike in wild-type mice
• however, no hemispheric difference in the degeneration is observed
|
|
• early in degeneration, membranous whorls are present on the retinal pigment epithelium unlike in wild-type mice
• however, by P45 the whorls have largely disappeared
• few, if any, phagosome are found in the retinal pigment epithelium at the peak period of rod outer disc shedding unlike in wild-type mice
• at 6 months of age, retinal capillaries invade the retinal pigment epithelium unlike in wild-type mice
• at 8 months, retinal pigment epithelium invades the retina and localizes along retinal blood vessels unlike in wild-type
|
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• at P45, small focal thinning in the retinal pigment epithelium is observed unlike in wild-type mice
• however, some retinal pigment epithelium is thicker than normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the 100% lethal dose (LD100) of LPS-treated mice exhibit is half the concentration as the LD100 of similarly treated wild-type mice
• however, pre-treatment of mice with anti-TNF-alpha antibodies prior to LPS treatment protects them from lethal sepsis
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• serum TNF-alpha levels in LPS treated mice are 3-fold higher than in similarly treated wild-type mice
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• LPS-treated macrophages secrete more TNF-alpha than similarly treated wild-type cells
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• LPS-treated macrophages secrete more TNF-alpha than similarly treated wild-type cells
• the 100% lethal dose (LD100) of LPS-treated mice exhibit is half the concentration as the LD100 of similarly treated wild-type mice
• LPS-treated mice exhibit increased lesions and hemorrhagic necrosis in the bowel with greater fluid accumulation, an increase infiltration of red blood cells and leukocytes into the villi, and degeneration of villi compared to in similarly-treated wild-type mice
• serum TNF-alpha levels in LPS treated mice are 3-fold higher than in similarly treated wild-type mice
• however, pre-treatment of mice with anti-TNF-alpha antibodies prior to LPS treatment protects them from lethal sepsis
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• LPS-treated mice exhibit increased lesions and hemorrhagic necrosis in the bowel with greater fluid accumulation, an increase infiltration of red blood cells and leukocytes into the villi, and degeneration of villi compared to in similarly-treated wild-type mice
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• serum TNF-alpha levels in LPS treated mice are 3-fold higher than in similarly treated wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Mononuclear cell infiltration of the large intestines and salivary glands are reduced in Mertktm1Gkm/Mertktm1Gkm mice
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• thymic dendritic cells pulsed with BDC induce more apoptosis in cocultured NOD thymocytes expressing Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi compared to heterozygous cells without altering phenotypic markers for dendritic cell activation and maturation
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• few mice develop insulitis by 12 and 17 weeks of age
• infiltrate into islet cells is composed of mostly B cells and CD8+ T cells with few CD4+ T cells unlike in heterozygous mice
• beta cell-specific T cells are reduced in the islet cells compared to in heterozygous mice
• at 12 weeks of age, the frequency of islet-infiltrating CD4+ and CD8+ T cells secreting IFN-gamma is reduced compared to in heterozygotes
• at 12 weeks, the frequency of NRP-V7-specific CD8+ T cells in islet infiltrate is decreased compared to in heterozygous mice
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• mice exhibit reduced mononuclear cell infiltrate in the large intestines and salivary glands compared to in NOD mice
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• beta cell-specific T cells are reduced in the islet cells compared to in heterozygous mice
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• female mice do not develop diabetes by 52 weeks of age unlike heterozygous mice
• when bone marrow cells are used to reconstitute irradiated NOD mice recipients remain diabetes free
• however, irradiated mice reconstituted with NOD bone marrow develop diabetes
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• few mice develop insulitis by 12 and 17 weeks of age
• infiltrate into islet cells is composed of mostly B cells and CD8+ T cells with few CD4+ T cells unlike in heterozygous mice
• beta cell-specific T cells are reduced in the islet cells compared to in heterozygous mice
• at 12 weeks of age, the frequency of islet-infiltrating CD4+ and CD8+ T cells secreting IFN-gamma is reduced compared to in heterozygotes
• at 12 weeks, the frequency of NRP-V7-specific CD8+ T cells in islet infiltrate is decreased compared to in heterozygous mice
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• thymic dendritic cells pulsed with BDC induce more apoptosis in cocultured NOD thymocytes expressing Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi compared to heterozygous cells without altering phenotypic markers for dendritic cell activation and maturation
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• mice exhibit reduced mononuclear cell infiltrate in the large intestines and salivary glands compared to in NOD mice
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• beta cell-specific T cells are reduced in the islet cells compared to in heterozygous mice
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• thymic dendritic cells pulsed with BDC induce more apoptosis in cocultured NOD thymocytes expressing Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi compared to heterozygous cells without altering phenotypic markers for dendritic cell activation and maturation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• pan-retinal fundus patches
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• severe photoreceptor loss in the peripheral retina
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• slight thinning
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• severe photoreceptor loss in the peripheral retina
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the frequency of double positive T cell apoptosis in thymic lobes is increased compared to in similarly treated heterozygous mice
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• fetal thymic organ cultures treated with BDC2.5 produce fewer double positive thymocytes than similarly treated heterozygous cells
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• fetal thymic organ cultures treated with BDC2.5 produce more CD8+ single positive thymocytes than similarly treated heterozygous cells
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• the frequency of double positive T cell apoptosis in thymic lobes is increased compared to in similarly treated heterozygous mice
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• fetal thymic organ cultures treated with BDC2.5 produce fewer double positive thymocytes than similarly treated heterozygous cells
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• fetal thymic organ cultures treated with BDC2.5 produce more CD8+ single positive thymocytes than similarly treated heterozygous cells
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• the frequency of double positive T cell apoptosis in thymic lobes is increased compared to in similarly treated heterozygous mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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