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Allele Symbol Allele Name Allele ID |
Stk11tm1.2Rdp targeted mutation 1.2, Ronald DePinho MGI:2387403 |
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| Summary |
8 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• only 25% of mouse embryonic fibroblasts (MEFs) spontaneously differentiate into myofibroblasts compared to 75% of wild-type MEFs
• following 12 hours of culture, only 15.9% of MEFs exhibit high contraction compared to 53.2% of wild-type MEFs
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• lethality manifests at about E8.5-E11
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• placental development is abnormal
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• embryos display defects in vasculogenesis
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• at low densities, cells do not form colonies efficiently
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• MEFs prepared from mutant embryos cultured under 3T9 or 3T3 protocols show a consistent increase in G1 content and a reciprocal reduction in G2
• MEFs fail to undergo growth arrest at later passages in contrast to wild-type and heterozygous MEFs, showing unabated growth after 40 doublings
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die at average age of 12.5 months
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• 100% of animals have gastrointestinal polyps at 11 months
• average polyp size is larger than in Stk11-conditional mutants
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• tumor formation (polyposis) in the gastrointestinal tract is increased greatly relative to controls
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• tumor formation (polyposis) in the gastrointestinal tract is increased greatly relative to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 5-7 day-old mice treated with one dose of DMBA succumb to skin and lung cancers (18/42 mice) with median survival of 28.6 weeks compared to wild-type mice which remain cancer-free for >40 weeks
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• polyps are present throughout gastrointestinal tract, including the stomach and colon
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• at 43 weeks, mice present with gastrointestinal obstruction
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• one animal exhibited a benign serous cyadenoma of the pancreas, and 2 showed asymptomatic benign uterine epithelial tumors, but no other neoplasms were observed in mutants
(J:78818)
• mice have reduced cancer-free survival (28.6 weeks) relative to wild-type (>40 weeks)
(J:131037)
• mutants are sensitized to squamous cell carcinoma compared to wild-type
(J:131037)
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• 5-7 day-old mice treated with one dose of DMBA (to promote development of lymphomas, sarcomas and lung adenomas) develop invasive skin and lung cancers not seen in wild-type mice
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• polyps present as mucosal hamartomas without dysplastic or adenomatous changes
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• 5-7 day-old mice treated with one dose of DMBA (to promote development of lymphomas, sarcomas and lung adenomas) develop invasive skin and lung cancers not seen in wild-type mice
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| Peutz-Jeghers syndrome | DOID:3852 |
OMIM:175200 |
J:78818 , J:131037 | |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 53% of female mice that survive to 55 weeks of age have endometrial adenocarcinomas that developed spontaneously
• tumors consisted of endometrial-type glands invading into the myometrium
• tumors maintained polarity of cells found in normal endometrial glands
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• 40 of 59 mice develop gastrointestinal polyps by 43 weeks of age
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• 53% of female mice that survive to 55 weeks of age have endometrial adenocarcinomas that developed spontaneously
• tumors consisted of endometrial-type glands invading into the myometrium
• tumors maintained polarity of cells found in normal endometrial glands
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in the lungs of mice treated with cre-expressing adenovirus
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• in the lungs of mice treated with cre-expressing adenovirus
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice inoculated with an adenovirus expressing cre recombinase (ad-cre) by inhalation have a mean survival of 9 weeks after ad-cre inoculation
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• regional lymph-node metastases and axial skeleton metastases are seen in 61% of ad-cre inoculated mice
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• mice inoculated with ad-cre develop lung tumors; tumor burden is increased at early time points and larger lesions at later time points are seen than in single Kras mutants
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• adenocarcinoma is seen in all metastatic lesions
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• 2 of 27 ad-cre treated mice show large cell carcinoma
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• 56% of ad-cre treated mice develop squamous cell carcinomas (SCCs) or adenosquamous carcinomas
• squamous tumors do not express pro-surfactant protein, but express pan-keratin and p63, markers of SCC
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• mice inoculated with ad-cre develop lung tumors; tumor burden is increased at early time points and larger lesions at later time points are seen than in single Kras mutants
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• adenocarcinoma is seen in all metastatic lesions
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• 2 of 27 ad-cre treated mice show large cell carcinoma
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• 56% of ad-cre treated mice develop squamous cell carcinomas (SCCs) or adenosquamous carcinomas
• squamous tumors do not express pro-surfactant protein, but express pan-keratin and p63, markers of SCC
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| lung non-small cell carcinoma | DOID:3908 | J:124682 | ||
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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