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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Pax5tm3Mbu
targeted mutation 3, Meinrad Busslinger
MGI:2386577
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Pax5tm3Mbu/Pax5tm3Mbu involves: 129P2/OlaHsd * C57BL/6 MGI:3720349
cn2
Cd19tm1(cre)Cgn/Cd19+
Pax5tm1Mbu/Pax5tm3Mbu
involves: 129P2/OlaHsd * 129S2/SvPas MGI:3720352
cn3
Pax5tm1Mbu/Pax5tm3Mbu
Tg(Mx1-cre)1Cgn/?
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA MGI:3720351
cn4
Pax5tm3Mbu/Pax5+
Tcf3tm1(PBX1)Mlc/Tcf3+
Cd19tm1(cre)Cgn/Cd19+
involves: 129P2/OlaHsd * C57BL/6 MGI:5825360
cn5
Pax5tm3Mbu/Pax5tm3Mbu
Tg(Gad1-cre,-lacZ)1Mini/0
involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:5486579
cn6
Pax5tm3Mbu/Pax5+
Tg(Gad1-cre,-lacZ)1Mini/0
involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:5486580


Genotype
MGI:3720349
hm1
Allelic
Composition
Pax5tm3Mbu/Pax5tm3Mbu
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice were indistinguishable from wild-type mice




Genotype
MGI:3720352
cn2
Allelic
Composition
Cd19tm1(cre)Cgn/Cd19+
Pax5tm1Mbu/Pax5tm3Mbu
Genetic
Background
involves: 129P2/OlaHsd * 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (8 available); any Cd19 mutation (15 available)
Pax5tm1Mbu mutation (1 available); any Pax5 mutation (19 available)
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells
• IgG class switching is decreased
• short-lived IgM-B220lo and IgM+B220lo immature B cells are slightly lower in number
• there is a 3-fold reduction in the number of long-lived mature B cells
• mice lack recirculating IgM+IgD+B220hi cells in the bone marrow
• short-lived IgM-B220lo B cells are slightly lower in number
• total immunoglobin is reduced 3-fold compared to control mice
• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels

hematopoietic system
• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells
• IgG class switching is decreased
• short-lived IgM-B220lo and IgM+B220lo immature B cells are slightly lower in number
• there is a 3-fold reduction in the number of long-lived mature B cells
• mice lack recirculating IgM+IgD+B220hi cells in the bone marrow
• short-lived IgM-B220lo B cells are slightly lower in number
• total immunoglobin is reduced 3-fold compared to control mice
• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels




Genotype
MGI:3720351
cn3
Allelic
Composition
Pax5tm1Mbu/Pax5tm3Mbu
Tg(Mx1-cre)1Cgn/?
Genetic
Background
involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm1Mbu mutation (1 available); any Pax5 mutation (19 available)
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
Tg(Mx1-cre)1Cgn mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• half of the mature B cells in the spleen are absent
• only a small number of mature B cells are lost upon short-term inactivation of Pax5
• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220hi cells

hematopoietic system
• half of the mature B cells in the spleen are absent
• only a small number of mature B cells are lost upon short-term inactivation of Pax5
• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220hi cells




Genotype
MGI:5825360
cn4
Allelic
Composition
Pax5tm3Mbu/Pax5+
Tcf3tm1(PBX1)Mlc/Tcf3+
Cd19tm1(cre)Cgn/Cd19+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (8 available); any Cd19 mutation (15 available)
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
Tcf3tm1(PBX1)Mlc mutation (0 available); any Tcf3 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• mice exhibit increased penetrance and accelerated acute leukemia development compared to single Tcf3tm1(PBX1)Mlc conditional mutants

hematopoietic system
• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation
• preleukemic mice show expansion of GFP+ progenitor cells of the Lin-CD19+CD43+ fractions at younger ages compared to single Tcf3tm1(PBX1)Mlc conditional mutants, indicating accelerated preleukemic progenitor B cell expansion
• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation

immune system
• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation
• preleukemic mice show expansion of GFP+ progenitor cells of the Lin-CD19+CD43+ fractions at younger ages compared to single Tcf3tm1(PBX1)Mlc conditional mutants, indicating accelerated preleukemic progenitor B cell expansion
• preleukemic mice show a greater decrease of immature B cells of the Lin-CD19+CD43- fractions compared to Tcf3tm1(PBX1)Mlc conditional mutants, indicating a more severe block of B cell differentiation at this stage of maturation




Genotype
MGI:5486579
cn5
Allelic
Composition
Pax5tm3Mbu/Pax5tm3Mbu
Tg(Gad1-cre,-lacZ)1Mini/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
Tg(Gad1-cre,-lacZ)1Mini mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die 1-2 weeks after macroscopic changes appear (ie. domed skulls); life expectancy of such mutants is 58 days; mice that do not show this phenotype survive at least 12 months and are fertile

skeleton
• 47% of mice show dome shaped skulls by about postnatal day 50

craniofacial
• 47% of mice show dome shaped skulls by about postnatal day 50

behavior/neurological
• mutants spend less time in the center in open-field tests, suggesting increased anxiogenic-like behavior
• in a novel open-field test, mutants with dome-shaped heads show significantly increased locomotion (likely due to the novelty aspect), as mutants appear lethargic in the home cages

nervous system
• 47% of mice
• prominent, varying mild to severe
• in most severe cases, medial structures between ventricles are deformed and compressed, as represented by vertically relocated hippocampi
• ventricular dilation might be result of obstruction of interventricular connections, increased CSF production by ependymal cells of the choroid plexus, or decreased CSF resorption by arachnoid villi
• other ventricles do not show enlargement
• cortex surrounding lateral ventricles is markedly thinner than in controls




Genotype
MGI:5486580
cn6
Allelic
Composition
Pax5tm3Mbu/Pax5+
Tg(Gad1-cre,-lacZ)1Mini/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm3Mbu mutation (1 available); any Pax5 mutation (19 available)
Tg(Gad1-cre,-lacZ)1Mini mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die 1-2 weeks after macroscopic changes appear (ie. domed skulls); life expectancy of such mutants is 58 days; mice that do not show this phenotype survive at least 12 months and are fertile

skeleton
• 19% of mice show dome shaped skulls by about postnatal day 50

craniofacial
• 19% of mice show dome shaped skulls by about postnatal day 50

behavior/neurological
• mutants spend less time in the center in open-field tests, suggesting increased anxiogenic-like behavior
• in a novel open-field test, mutants with dome-shaped heads show significantly increased locomotion (likely due to the novelty aspect), as mutants appear lethargic in the home cages

nervous system
• 19% of mice
• prominent, varying mild to severe





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last database update
03/31/2020
MGI 6.15
The Jackson Laboratory