Phenotypes associated with this allele

• Allele Symbol: Slc18a2^tm1Mca
• Allele Name: targeted mutation 1, Marc G Caron
• Allele ID: MGI:2386330
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc18a2^tm1Mca/Slc18a2^tm1Mca	involves: 129X1/SvJ	MGI:3852383
ht2	Slc18a2^tm1Mca/Slc18a2^+	B6.129X1-Slc18a2^tm1Mca	MGI:3852389
ht3	Slc18a2^tm1Mca/Slc18a2^+	involves: 129X1/SvJ	MGI:3852385
ht4	Slc18a2^tm1Mca/Slc18a2^+	involves: 129X1/SvJ * C57BL/6J	MGI:3852390

Genotype
MGI:3852383

hm1

• Allelic Composition: Slc18a2^tm1Mca/Slc18a2^tm1Mca
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:45184 )

• most die within 1 to 3 days of birth

• some mice survive to P12

nervous system

abnormal synaptic neurotransmitter level ( J:45184 )

• significant decrease in monoamine levels in the brain

homeostasis/metabolism

decreased body temperature ( J:45184 )

• newborns are more prone to hypothermia compared to controls

abnormal dopamine level ( J:45184 )

• following electrical stimulation of striatal slices no measurable dopamine overflow is seen, unlike in wild-type mice

abnormal enzyme/coenzyme activity ( J:45184 )

• assays indicate elevated tyrosine hydroxylase and tryptophan hydroxylase activities in brains from newborn mice

growth/size/body

decreased birth body size ( J:45184 )

postnatal growth retardation ( J:45184 )

behavior/neurological

decreased locomotor activity ( J:45184 )

• seen in newborn mice

Genotype
MGI:3852389

ht2

• Allelic Composition: Slc18a2^tm1Mca/Slc18a2⁺
• Genetic Background: B6.129X1-Slc18a2^tm1Mca

nervous system

enhanced long-term potentiation ( J:89778 )

• significant enhancement of long term potentiation in a minimal tetanus assay

behavior/neurological

behavior/neurological phenotype ( J:125273 )

N • mice do not display abnormal anxiety like behaviors

abnormal associative learning ( J:125273 )

• impairment in learned helplessness behavior

abnormal liquid preference ( J:125273 )

• reduced sucrose-water to water preference ratios at 1% and 1.5% sucrose compared to wild-type controls

decreased coping response ( J:125273 )

• significant increase in immobility time in a forced swim test or a tail suspension test

• treatment with antidepressants decreases immobility times

decreased vertical activity ( J:125273 )

• in an open field test

decreased locomotor activity ( J:125273 )

• in an open field test

homeostasis/metabolism

abnormal corticosterone level ( J:125273 )

• increase in corticosterone levels under stressful conditions but not under basal conditions

Genotype
MGI:3852385

ht3

• Allelic Composition: Slc18a2^tm1Mca/Slc18a2⁺
• Genetic Background: involves: 129X1/SvJ

nervous system

abnormal neurotransmitter secretion ( J:45184 )

• marked reduction in amphetamine evoked striatal dopamine release in freely moving mice

abnormal neurotransmitter uptake ( J:45184 )

• about a 50% reduction in the initial rate of dopamine uptake in vesicular preparations from adult striata

behavior/neurological

impaired behavioral response to cocaine ( J:45184 )

• following chronic exposure to cocaine no increase in behavioral response is seen following a challenge dose unlike in wild-type mice

enhanced behavioral response to addictive substance ( J:45184 )

• marked increase in behavioral response to apomorphine, cocaine, and amphetamine compared to similarly treated wild-type mice

homeostasis/metabolism

decreased dopamine level ( J:45184 )

• dopamine content in the striata is about 25% lower than in wild-type mice, but the level of the dopamine metabolite, DOPAC, is significantly increased

• extracellular striatal dopamine levels are reduced by about 40% compared to wild-type mice

decreased physiological sensitivity to xenobiotic ( J:45184 )

• marked reduction in amphetamine evoked striatal dopamine release in freely moving mice

Genotype
MGI:3852390

ht4

• Allelic Composition: Slc18a2^tm1Mca/Slc18a2⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:124481 )

N • no difference in activity is seen in mice that consumed saline or alcohol compared to matched wild-type controls

decreased alcohol consumption ( J:124481 )

• male mice show a marked decrease in alcohol consumption compared to wild-type males

impaired conditioned place preference behavior ( J:124481 )

• mice do not display a preference for the environment paired with ethanol in a place preference assay

abnormal liquid preference ( J:124481 )

• slight but significant decrease in preference for saccharin water in males compared to wild-type males